1. Sucrose importation into laticifers of Hevea brasiliensis, in relation to ethylene stimulation of latex production
- Author
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Soulaiman Sakr, Panida Kongsawadworakul, Unchera Viboonjun, Nicole Brunel, Anaïs Dusotoit-Coucaud, Jean-Louis Julien, Hervé Chrestin, André Lacointe, Sciences Agronomiques Appliquées à l'Horticulture (SAGAH), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
- Subjects
0106 biological sciences ,Sucrose ,Ethylene ,MESH: Hevea ,Latex ,glutamine-synthetase ,Plant Science ,01 natural sciences ,MESH: Membrane Transport Proteins ,Trees ,chemistry.chemical_compound ,transcriptome analysis ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Gene expression ,ethylene ,walnut tree ,sieve elements ,laticifers ,Cloning, Molecular ,latex production ,MESH: Phylogeny ,[SDV.SA.HORT]Life Sciences [q-bio]/Agricultural sciences/Horticulture ,MESH: Organ Specificity ,In Situ Hybridization ,Phylogeny ,Plant Proteins ,0303 health sciences ,MESH: Plant Proteins ,plasma-membrane ,Sucrose transport ,Hevea brasiliensis ,sugar ,Organ Specificity ,visual_art ,uptake ,transporter ,visual_art.visual_art_medium ,Plant Bark ,Bark ,Amino Acid Oxidoreductases ,MESH: Plant Bark ,DNA, Complementary ,MESH: Ethylenes ,MESH: Biological Transport ,MESH: Amino Acid Oxidoreductases ,Molecular Sequence Data ,transporters ,Biology ,03 medical and health sciences ,MESH: Gene Expression Profiling ,MESH: In Situ Hybridization ,Glutamate-Ammonia Ligase ,Botany ,MESH: Glutamate-Ammonia Ligase ,MESH: Cloning, Molecular ,expression analysis ,RNA, Messenger ,MESH: Gene Expression Regulation, Plant ,Sugar ,MESH: Plant Growth Regulators ,030304 developmental biology ,MESH: RNA, Messenger ,MESH: Molecular Sequence Data ,Gene Expression Profiling ,MESH: Sucrose ,MESH: Latex ,Membrane Transport Proteins ,Biological Transport ,MESH: DNA, Complementary ,Original Articles ,Ethylenes ,biology.organism_classification ,gene-expression ,MESH: Trees ,chemistry ,Hevea ,rubber tree ,010606 plant biology & botany - Abstract
† Background and Aims The major economic product of Hevea brasiliensis is a rubber-containing cytoplasm (latex), which flows out of laticifers (latex cells) when the bark is tapped. The latex yield is stimulated by ethylene. Sucrose, the unique precursor of rubber synthesis, must cross the plasma membrane through specific sucrose transporters before being metabolized in the laticifers. The relative importance of sucrose transporters in determining latex yield is unknown. Here, the effects of ethylene (by application of Ethrel w ) on sucrose transporter gene expression in the inner bark tissues and latex cells of H. brasiliensis are described. † Methods Experiments, including cloning sucrose transporters, real time RT-PCR and in situ hybridization, were carried out on virgin (untapped) trees, treated or untreated with the latex yield stimulant Ethrel. † Key Results Seven putative full-length cDNAs of sucrose transporters were cloned from a latex-specific cDNA library. These transporters belong to all SUT (sucrose transporter) groups and differ by their basal gene expression in latex and inner soft bark, with a predominance of HbSUT1A and HbSUT1B. Of these sucrose transporters, only HbSUT1A and HbSUT2A were distinctly increased by ethylene. Moreover, this increase was shown to be specific to laticifers and to ethylene application. † Conclusion The data and all previous information on sucrose transport show that HbSUT1A and HbSUT2A are related to the increase in sucrose import into laticifers, required for the stimulation of latex yield by ethylene in virgin trees.
- Published
- 2009