1. [Methods for studying the in vitro susceptibility of Candida spp. to antifungals]
- Author
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Hayet Sallemi, S. Abbes, Sourour Nej, Ali Ayadi, I. Amouri, Houaida Trabelsi, Fattouma Makni, and Chaikhrouhou Fatma
- Subjects
Antifungal ,Susceptibility testing ,Antifungal Agents ,business.industry ,medicine.drug_class ,Candidiasis ,Colony Count, Microbial ,General Medicine ,Liquid medium ,Microbial Sensitivity Tests ,In Vitro Techniques ,Flow Cytometry ,Candida infections ,Microbiology ,Europe ,Clinical microbiology ,Serum Bactericidal Test ,Disk Diffusion Antimicrobial Tests ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Candida spp ,Medicine ,Humans ,business ,Candida - Abstract
In recent years, an increase of systemic Candida infections was noted. Thus, identification and susceptibility testing to antifungals became of considerable importance. The technique of dilution in liquid medium developed by « National committee for clinical laboratory standards » NCCLS or more recently named CLSI « Clinical and laboratory standards institute » is the reference method most used. The European committee "European committee on antibiotic susceptibility testing" or EUCAST has made progress by determining the susceptibility of strains within a shorter time. However, the use of these techniques is limited especially in clinical microbiology laboratories. Other techniques for determining antifungal sensitivity have been developed such as those based on agar diffusion (E-test and disk diffusion), on microdilution (Sensititre yeastOne, Vitek 2 AST-YS01), on flow cytometry techniques and the MALDI-TOF.
- Published
- 2012