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Background: The lung tissue in newborn canine neonates is still in a morphologically and functionally immature, canalicular-saccular stage. True alveoli are only formed postnatally. The aim of this study was to analyze the spatial and temporal development of the ventilation of the lung tissue in vital canine neonates during the first 24 h post natum (p.n.)., Methods: Forty pups (birth weight Ø 424 g ± 80.1 g) from three litters of large dog breeds (>20 kg live weight) were included in the studies. Thirty-three pups (29 vital, 2 vitally depressed, 2 stillborn neonates) originated from controlled, uncomplicated births ( n = 3); moreover, six stillborn pups as well as one prematurely deceased pup were birthed by other dams with delivery complications. Computed tomography (CT) was used in 39 neonates, and histopathologic tissue classification techniques (HALO) were used in 11 neonates (eight stillborn and three neonates died early post natum, respectively) to quantify the degree of aerated neonatal lung tissue., Results: It was shown that, in vital born pups, within the first 10 min p.n., the degree of ventilation reached mean values of -530 (±114) Hounsfield units (HU) in the dorsal and -453.3 (±133) HU in the ventral lung area. This is about 75-80% of the final values obtained after 24 h p.n. for dorsal -648.0 (±89.9) HU and ventral quadrants -624.7 (±76.8) HU. The dorsal lung areas were always significantly better ventilated than the ventral regions ( p = 0.0013). CT as well as histopathology are suitable to clearly distinguish the nonventilated lungs of stillborns from neonates that were initially alive after surviving neonatal respiratory distress syndrome but who died prematurely ( p = 0.0398)., Conclusion: The results of this study are clinically relevant since the lung tissue of canine neonates presents an aeration profile as early as 10 min after birth and continues progressively, with a special regard to the dorsal lung areas. This is the basis for resuscitation measures that should be performed, preferably with the pup in the abdomen-chest position.

Capture of wild boar in corral-style traps with subsequent culling is increasingly used for population management. The method is debated due to animal welfare concerns making welfare studies in traps necessary. While previous studies focused on behaviour and injuries, this study dealt with the physiological aspect. Cortisol levels in wild boar caught in corral-style traps (50-90 qm 2 , n = 138) were compared with those killed during single ( n = 37) and driven hunts ( n = 90). Collected sera were purified by solid phase extraction (SPE) and analysed via radioimmunoassay. Cortisol levels in blood samples were stable under cooled (4-7 °C) conditions for a storage time of up to 87 h before centrifugation. Cortisol levels were significantly higher in wild boar killed in corral-style traps than during driven hunts and single hunts. Wild boar caught in groups of five or more showed lower cortisol levels than single animals or in smaller groups. Therefore, time span inside the trap and of culling should be reduced to a minimum, and capturing groups of animals should be preferred to reduce stress. For animal welfare assessment of wild boar live-trapping, additional data from behavioural analyses and pathological examinations must be integrated.

Kinetic examinations of horses with induced lameness as well as the effect of perineural anaesthesia in sound horses have shown promise, but clinical studies regarding the effect of diagnostic anaesthesia during the different stance phases are rare. Fourteen horses with unilateral forelimb lameness were examined with the Hoof™ System during trot to assess vertical force distribution (in kg) affecting both front hooves before and after diagnostic anaesthesia during landing, midstance, and breakover. For statistical analysis, a covariance analysis with repeated measurements regarding the limb (lame/sound) as well as anaesthesia (before/after) and the covariable body weight was performed. The p-values for the pairwise comparisons were adjusted using the Bonferroni−Holm correction (p < 0.05). For all phases of the stance, a significant interaction between the factors limb and anaesthesia was shown. Before diagnostic anaesthesia, vertical force was significantly reduced on the lame limb compared to the sound limb during landing (−25%, p < 0.001), midstance (−20%, p < 0.001) and breakover (−27%, p < 0.001). After anaesthesia, the difference between both forelimbs was not significant anymore for all phases. The vertical force on the lame limb increased significantly after positive anaesthesia during the whole stance phase, with breakover being most affected (+27%, p = 0.001). Pressure measurements with the Hoof™ System can be used to evaluate the effect of diagnostic anaesthesia in a clinical setting with pain-related vertical force asymmetries being neutralised after diagnostic anaesthesia. Breakover is the main event influenced by lameness.

For dairy calves, sufficient supply with high-quality maternal colostrum is crucial to achieve adequate passive transfer of immunoglobulins. This observational cross-sectional study aimed to determine the influence of the prepartum metabolic status of dams on the serum immunoglobulin and total protein concentrations of their dairy calves, taking other relevant management factors into account. A total of 551 cows and their calves from 124 German dairy farms were included. Blood and urine samples of the cows were sampled 1 to 3 weeks before the expected calving date. Two generalized linear mixed effects regression models were fitted to the data. An increase in a dam's prepartum serum non-esterified fatty acids concentration was associated with greater serum immunoglobulin concentration in her calf. Calves of herds with established birth monitoring at night showed greater serum immunoglobulin and total protein concentrations. Calves being fed more than 2 L of colostrum and higher Brix values for colostrum were related to greater serum immunoglobulin and total protein concentrations in calves. In conclusion, there is evidence that, besides timely and sufficient supply of high-quality colostrum to new-born calves, the prepartum metabolic status of cows and birth monitoring impact the passive transfer of immunoglobulins.

The aim of this study was to analyze the utilization of different stable areas of a total of 52 group-housed horses as well as their preferred stable parts and the use of resources. The study was situated in a "HIT Active Stable ® " in Northern Germany for a period of 227 observation days. After dividing the whole farm area in a grid of 3 × 3 m, the dataset was examined with and without the pasture area. Furthermore, linear mixed models were applied. On average, horses used 53.2 ± 19 different squares per hour. The observation day ( p < 0.001) and the covariate age ( p < 0.001) had significant effects on the different squares visited per hour. No significant effects were found for sex ( p = 0.30) and breed ( p = 0.65) as only geldings and no stallions were part of the group and the distribution of the breeds was unfavorable. The random effect animal showed that the horse-individual estimates from -19.2 to 17.6 different squares visited per hour were quite large. Furthermore, it could be shown that the horses used resources such as feed stalls with a frequency of up to 0.14% more than other paddock areas without resources. Open lying halls with tarp skin were also preferred over the metal hall. The shelters were only partly popular. Use could be visualized with the help of heat maps. This study gives a good overview of the use of individual areas and resources and possible improvements.

Besides the direct transport of animals, also indirect transmission routes, e.g., contact via contaminated vehicles, have to be considered. In this study, the transmission routes of a German pig trade network were illustrated as a monopartite animal movements network and two bipartite networks including information of the transport company and the feed producer which were projected on farm level ( n = 866) to enable a comparison. The networks were investigated with the help of network analysis and formed the basis for epidemiological models to evaluate the impact of different transmission routes on network structure as well as on potential epidemic sizes. The number of edges increased immensely from the monopartite animal movements network to both projected networks. The median centrality parameters revealed clear differences between the three representations. Furthermore, moderate correlation coefficients ranging from 0.55 to 0.68 between the centrality values of the animal movements network and the projected transportation network were obtained. The epidemiological models revealed significantly more infected farms for both projected networks (70% to 100%) compared to the animal movements network (1%). The inclusion of indirect transmission routes had an immense impact on the outcome of centrality parameters as well as on the results of the epidemiological models.

Dominance indices are often calculated using the number of won and lost fights of each animal focusing on dyadic interactions. Social network analysis provides new insights into the establishment of stable group structures going beyond the dyadic approach. Thus, it was investigated whether centrality parameters describing the importance of each animal for the network are able to capture the rank order calculated by dominance indices. Therefore, two dominance indices and five centrality parameters based on two network types (initiator-receiver and winner-loser networks) were calculated regarding agonistic interactions observed in three mixing events (weaned piglets, fattening pigs, gilts). Comparing the two network types, the winner-loser networks demonstrated highly positive correlation coefficients between out-degree and outgoing closeness and the dominance indices. These results were confirmed by partial least squares structural equation modelling (PLS-SEM), i.e., about 60% of the variance of the dominance could be explained by the centrality parameters, whereby the winner-loser networks could better illustrate the dominance hierarchy with path coefficients of about 1.1 for all age groups. Thus, centrality parameters can portray the dominance hierarchy providing more detailed insights into group structure which goes beyond the dyadic approach.

Dominance indices are calculated by considering the differences between the number of won and lost fights. Whether these differences show a significant asymmetric outcome or not is neglected. Thus, two calculation methods for the limits of significant dyads are proposed using a sign test based on the differences in won and lost fights, considering all dyadic interactions in the pen (PEN: pen individual limits), and a sign test focusing on each individual dyad (DYAD: dyad individual limits). These were compared to the data set containing all dyadic interactions (ALL). Agonistic interactions in three mixing events were video recorded for two and a half days (weaned piglets) or one and a half days (fattening pigs, gilts). Dominance indices (DI) were calculated for all data sets. Pen/dyad individual limits revealed a small number of significant dyads (weaned piglets: 12.4%/8.8%; fattening pigs: 4.2%/0.6%; gilts: 3.6%/0.4%). Pen individual limits should be selected as they allow adaption of the limits according to the fighting frequency. Spearman rank correlation coefficients of the dominance indices between the data sets were always above 0.7, implying that the rank order remained relatively stable. Information about the impact of significant dyads on sociometric measures is important to prevent misinterpretations about the social structure in animal groups and should be considered in future studies.

To enhance feasibility, the Animal Welfare Indicators (AWIN) assessment protocol for horses consists of two levels: the first is a visual inspection of a sample of horses performed from a distance, the second a close-up inspection of all horses. The aim was to analyse whether information would be lost if only the first level were performed. In this study, 112 first and 112 second level assessments carried out on a subsequent day by one observer were compared by calculating the Spearman's Rank Correlation Coefficient (RS), Intraclass Correlation Coefficients (ICC), Smallest Detectable Changes (SDC) and Limits of Agreements (LoA). Most indicators demonstrated sufficient reliability between the two levels. Exceptions were the Horse Grimace Scale, the Avoidance Distance Test and the Voluntary Human Approach Test (e.g., Voluntary Human Approach Test: RS: 0.38, ICC: 0.38, SDC: 0.21, LoA: -0.25-0.17), which could, however, be also interpreted as a lack of test-retest reliability. Further disagreement was found for the indicator consistency of manure (RS: 0.31, ICC: 0.38, SDC: 0.36, LoA: -0.38-0.36). For these indicators, an adaptation of the first level would be beneficial. Overall, in this study, the division into two levels was reliable and might therewith have the potential to enhance feasibility in other welfare assessment schemes., Competing Interests: The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.