Search

Simple Summary: Parental obesity affects skeletal metabolism in offspring. This relationship is called "nutritional programming". During the weaning period, they are more highly mineralized and mechanically resistant. It was interesting for us whether changing or continuing the feeding of male offspring with a standard or high-energy diet may have different metabolic effects on bone tissue. Our previous studies on females have shown that the beneficial direction of change is the replacement of the standard diet with a high-energy diet; the reduction of the caloric content of food (change from a high-energy to a properly balanced diet) leads to disorders of skeletal growth and development. In males, any change in diet inhibited skeletal development, and the bones were weaker. The most effective was the continuation of high-energy nutrition, which, in males at 49 and 90 days of age, was manifested by stronger bones. This proves that males and females react differently to a change in the caloric content of the diet during the period of growth and development. The experiment was undertaken to assess whether the continuation or change of the parents' diet affects the previously programmed bone metabolism of the male offspring during its growth and development. A total of 16 male and 32 female Wistar rats were divided into groups and fed a standard (diet S) or high-energy (diet F). After the induction of obesity, the rats from groups S and F, as the parent generation, were used to obtain male offspring, which were kept with their mothers until the weaning day (21 days of age). In our earlier study, we documented the programming effects of the diet used in parents on the skeletal system of offspring measured on the weaning day. Weaned male offspring constitute one control group—parents and offspring fed the S diet. There were three experimental groups, where: parents received diet S and offspring were fed with the F diet; parents were treated with the diet F, while offspring received the S diet; and parents and offspring were fed with the diet F. The analyses were performed at 49 and 90 days of life. After sacrifice, cleaned-off soft tissue femora were assessed using peripheral quantitative computed tomography (pQCT), dual X-ray absorptiometry (DXA), and a three-point bending test. We observed that changing and continuation of nutrition, applied previously in parents, significantly influenced the metabolism of the bone tissue in male offspring, and the osteotropic effects differed, depending on the character of the nutrition modification and age. Additionally, an important conclusion of our study, regarding the previous, is that nutrition modification, affecting the metabolism of bone tissue, also depends on the sex. [ABSTRACT FROM AUTHOR]

Simple Summary: Overweight and obesity can cause many diseases, and several studies indicate a close relationship between the obesity of parents and the health of their offspring. Our aim was to investigate whether there is a programming influence of parental obesity on the skeletal system in weaned female and male offspring rats. In undertaking this, analysis of bone material was carried out using isolated tibia, and densitometry (DXA), peripheral (pQCT) and micro (µCT) computed tomography were performed. Mechanical tests and blood serum biochemistry were also carried out. Our work showed a significant programming influence of parental obesity on neonatal skeletal development. The tibiae isolated from offspring originating from obese parents were characterized by more intense mineralization and higher fracture resistance. However, numerous studies demonstrate the destructive effect of obesity on the skeletal system. Our research and the available literature suggest the existence of a "fat threshold", the exceeding of which changes of the osteotropic effect of adipose tissue to become unfavorable. Therefore, there is a need for further research to determine the time-dependent metabolic relationship between adipose tissue and bone in both animals and humans. Our study aimed to verify the hypothesis of the existence of a programming effect of parental obesity on the growth, development and mineralization of the skeletal system in female and male rat offspring on the day of weaning. The study began with the induction of obesity in female and male rats of the parental generation, using a high-energy diet (group F). Females and males of the control group received the standard diet (group S). After 90 days of dietary-induced obesity, the diet in group F was changed into the standard. Rats from groups F and S were mated to obtain offspring which stayed with their mothers until 21 days of age. Tibia was tested using dual-energy X-ray absorptiometry (DXA), peripheral quantitative computed tomography (pQCT), micro-computed tomography (µCT) and mechanical strength using the three-point bending test. Biochemical analysis of blood serum bone metabolism markers was performed. DXA analysis showed higher tibia bone mineral content (BMC) and area. pQCT measurements of cortical and trabecular tissue documented the increase of the volumetric bone mineral density and BMC of both bone compartments in offspring from the F group, while µCT of the trabecular tissue showed an increase in trabecular thickness and a decrease of its separation. Parental obesity, hence, exerts a programming influence on the development of the skeletal system of the offspring on the day of the weaning, which was reflected in the intensification of mineralization and increased bone strength. [ABSTRACT FROM AUTHOR]