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On page 24, Section 3.1.2, first paragraph, line 15, the citation [408] should be changed to [411] On page 24, Section 3.1.2, first paragraph, line 16, the citation [365] should be changed to [367]. On page 23, Section 3.1.1, second paragraph, line 11, the citation for "...Atlantic salmon, 0.6 (Lall and Bishop, 1977)" should be changed as "...Atlantic salmon, 0.6 [225]". Text Corrections On page 4, Section 2.1, first paragraph, line 8, the word "monooxygenase" should be revised to "monoxygenase". [Extracted from the article]

Reference 1 Ferosekhan S., Turkmen S., Pérez-García C., Xu H., Gómez A., Shamna N., Afonso J.M., Rosenlund G., Fontanillas R., Gracia A. Influence of Genetic Selection for Growth and Broodstock Diet n-3 LC-PUFA Levels on Reproductive Performance of Gilthead Seabream, Sparus aurata. Funding This study was funded by the EU-funded project AQUAIMPACT (Genomic and Nutritional Innovations for Genetically Superior Farmed Fish), grant agreement No. 818367, PROGENSA III (Mejora de la competitividad del sector de la dorada a través de la selección genética, JACUMAR program with support from the European Maritime and Fisheries), and the Indian Council of Agricultural Research (ICAR), New Delhi, India. Influence of Genetic Selection for Growth and Broodstock Diet n-3 LC-PUFA Levels on Reproductive Performance of Gilthead Seabream, Sparus Aurata. [Extracted from the article]

Simple Summary: Dietary hydroxy-selenomethionine (OH-SeMet) reduces oxidative stress and modulates immune response against bacterial infection in fish. However, the effect of OH-SeMet on essential fatty acids with a high oxidation risk or on the response against viral infection has not been sufficiently studied. This study aimed to assess the impact of dietary OH-SeMet supplementation on whole-body fatty acid profiles and response against viral infection. Gilthead seabream (Sparus aurata) juveniles were fed for 91 days with three experimental diets, a control diet without Se supplementation (0.29 mg Se kg diet−1) and two diets supplemented with OH-SeMet (0.52 and 0.79 mg Se kg diet−1). Afterwards, a crowding stress challenge and an anti-viral response challenge were conducted. Selenium (Se), proximate and fatty acid composition of diets and body tissues were analyzed, as well as plasma cortisol and the antiviral response protein Mx gene expression. Elevation in dietary Se (from 0.29 to 0.79 mg kg−1) proportionally raised Se contents in body tissues (from 0.79 to 1.35 mg kg−1), increased lipid contents in whole body (from 9.46 to 10.83%), and promoted the retention and synthesis of n-3 polyunsaturated fatty acids (from 44.59 to 72.91%), reducing monounsaturated (from 44.07 to 42.00 %) and saturated fatty acids (29.77 to 26.92 %) contents in whole-body lipids. Additionally, it increased 2 h post-stress plasma cortisol levels and after poly I:C injection up-regulated Mx and other immune response related genes, showing, for the first time in gilthead seabream, the importance of dietary Se levels on antiviral defense. The supplementation of fish diets with OH-SeMet reduces oxidative stress and modulates immune response against bacterial infection. However, despite the importance of essential polyunsaturated fatty acids in fish nutrition and their high risk of oxidation, the potential protective effect of OH-SeMet on these essential fatty acids has not been studied in detail. Moreover, while viral infection is very relevant in seabream production, no studies have focused the Se effects against viral infection. The aim of the present study was to assess the impact of dietary supplementation with OH-SeMet on gilthead seabream fatty acid profiles, growth performance and response against viral infection. Gilthead seabream juveniles (21.73 ± 0.27 g) were fed for 91 days with three experimental diets, a control diet without supplementation of Se (0.29 mg Se kg diet−1) and two diets supplemented with OH-SeMet (0.52 and 0.79 mg Se kg diet−1). A crowding stress test was performed at week 7 and an anti-viral response challenge were conducted at the end of the feeding trial. Selenium, proximate and fatty acid composition of diets and body tissues were analyzed. Although fish growth was not affected, elevation in dietary Se proportionally raised Se content in body tissues, increased lipid content in the whole body and promoted retention and synthesis of n-3 polyunsaturated fatty acids. Specifically, a net production of DHA was observed in those fish fed diets with a higher Se content. Additionally, both monounsaturated and saturated fatty acids were significantly reduced by the increase in dietary Se. Despite the elevation of dietary Se to 0.79 mg kg−1 not affecting basal cortisol levels, 2 h post-stress plasma cortisol levels were markedly increased. Finally, at 24 h post-stimulation, dietary OH-SeMet supplementation significantly increased the expression of the antiviral response myxovirus protein gene, showing, for the first time in gilthead seabream, the importance of dietary Se levels on antiviral defense. [ABSTRACT FROM AUTHOR]

Simple Summary: Our aim is to introduce the mineral nutrition of fish and explain the complexity of determining requirements for these elements, which are absorbed and excreted by the fish into the surrounding water. To date, only the requirements for nine minerals have been investigated. The review is focused on the absorption and the dietary factors that reduce their absorption from feed ingredients of plant and animal origin. Some diseases, such as cataracts, anemia and bone deformity, have been linked to dietary deficiency of minerals. Aquatic animals have unique physiological mechanisms to absorb and retain minerals from their diets and water. Research and development in the area of mineral nutrition of farmed fish and crustaceans have been relatively slow and major gaps exist in the knowledge of trace element requirements, physiological functions and bioavailability from feed ingredients. Quantitative dietary requirements have been reported for three macroelements (calcium, phosphorus and magnesium) and six trace minerals (zinc, iron, copper, manganese, iodine and selenium) for selected fish species. Mineral deficiency signs in fish include reduced bone mineralization, anorexia, lens cataracts (zinc), skeletal deformities (phosphorus, magnesium, zinc), fin erosion (copper, zinc), nephrocalcinosis (magnesium deficiency, selenium toxicity), thyroid hyperplasia (iodine), muscular dystrophy (selenium) and hypochromic microcytic anemia (iron). An excessive intake of minerals from either diet or gill uptake causes toxicity and therefore a fine balance between mineral deficiency and toxicity is vital for aquatic organisms to maintain their homeostasis, either through increased absorption or excretion. Release of minerals from uneaten or undigested feed and from urinary excretion can cause eutrophication of natural waters, which requires additional consideration in feed formulation. The current knowledge in mineral nutrition of fish is briefly reviewed. [ABSTRACT FROM AUTHOR]

Simple Summary: Gilthead seabream (GSB) broodstock were genetically selected based on their growth trait either high growth (HG) or low growth (LG) to evaluate the reproductive performance of these both traits under either fish oil (FO) or vegetable oil (VO) based diets feeding regime. The egg and larval quality were significantly improved by the broodstock selected for HG trait fed under FO based diet. This indicates that broodstock selected with HG trait has positive influence on the improving sperm, egg and larval quality in gilthead seabream. Genetic selection in gilthead seabream (GSB), Sparus aurata, has been undertaken to improve the growth, feed efficiency, fillet quality, skeletal deformities and disease resistance, but no study is available to delineate the effect of genetic selection for growth trait on GSB reproductive performance under mass spawning condition. In this study, high growth (HG) or low growth (LG) GSB broodstock were selected to evaluate the sex steroid hormones, sperm, egg quality and reproductive performance under different feeding regime of commercial diet or experimental broodstock diet containing either fish oil (FO) or vegetable oil (VO) based diet. Under commercial diet feeding phase, broodstock selected for either high growth or low growth did not show any significant changes in the egg production per kg female whereas egg viability percentage was positively (p = 0.014) improved by the high growth trait broodstock group. The experimental diet feeding results revealed that both growth trait and dietary fatty acid composition influenced the reproductive performance of GSB broodstock. In the experimental diet feeding phase, we observed high growth trait GSB males produced a higher number of sperm cells (p < 0.001) and also showed a higher sperm motility (p = 0.048) percentage. The viable egg and larval production per spawn per kg female were significantly improved by the broodstock selected for high growth trait and fed with fish oil-based diet. This present study results signifies that gilthead seabream broodstock selected on growth trait could have positive role in improvement of sperm and egg quality to produce viable progeny. [ABSTRACT FROM AUTHOR]