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Simple Summary: This present research work was designed for the validation of some SNPs of casein in Bos indicus (Sahiwal breed), previously reported for their association with milk yield in Bos taurus. This study indicated that these SNPs of casein genes (rs43703010, rs109500451, rs110079521, rs110323127, rs43703015, rs43703016 and rs43703017) significantly affected milk yield in Bos indicus of the Sahiwal breed. Animals with the genotype GG, having the SNP α S1 and aA S2 casein genes (rs43703010 and rs109500451), show high milk yield. For the SNPs of the κ casein genes (CSN3) (rs110079521, rs43703016 and rs43703017), animals with genotype AA were high milk producers. For the other two SNPs of the CSN3 gene (rs110323127 and rs43703015), animals with genotypes GG and CC produced a high milk yield. In light of these results, animal breeders may be able to make a more precise selection of the animals of Bos indicus (Sahiwal breed) with high milk yield. The aim of the present study was the validation of the already reported Bos taurus SNPs in the Sahiwal breed. A total of nine SNPs of the casein gene were studied. Out of nine, seven Bos taurus SNPs of casein protein genes were found to be significantly associated with milk productivity traits. The genomic DNA was extracted from the mammary alveolar endothelial cells of a flock of 80 purebred Sahiwal lactating dams available at Khizrabad Farm near Sargodha. New allele-specific primers were designed from the NCBI annotated sequence database of Bos taurus to obtain 100 nt-long PCR products. Each dam was tested separately for all the SNPs investigated. Animals with genotype GG for the SNPs rs43703010, rs10500451, and 110323127, respectively, exhibited high milk yield. Similarly, animals with genotype AA for the SNPs rs11079521, rs43703016, and rs43703017 showed high milk yield consistently. For the SNP rs43703015, animals with genotype CC showed high milk productivity. These above-mentioned SNPs have previously been reported to significantly up-regulate casein protein contents in Bos taurus. Our results indicated SNPs that significantly affect the milk protein contents may also significantly increase per capita milk yield. These finding suggest that the above-mentioned reported SNPs can also be used as genetic markers of milk productivity in Sahiwal cattle. [ABSTRACT FROM AUTHOR]