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Simple Summary: The situation in which our society finds itself regarding the excessive use of plastic materials in all areas is worrying due to the environmental pollution it generates, and the veterinary field is no exception to this situation, since the use of plastic is widespread in daily work. The replacement of plastic materials with more sustainable ones is urgent, and that is why the search for these elements is so important and a starting point for collaboration in the reduction in waste. Artificial insemination is a technique frequently used with equine species and involves the use of plastic materials such as insemination catheters and semen straws. Finding alternative biodegradable materials is the aim of this work, evaluating the use of straws made of bamboo, avocado, grass, paper, Kraft paper, wheat, and rice. The materials that offered the best results as containers for semen samples were bamboo and avocado, the latter being able to maintain the quality parameters of the sperm it contained without the presence of contamination, so we could consider it a viable alternative to the use of conventional plastic straws, thus contributing to a reduction in waste. Currently, plastic is used being without any limitations. The livestock sector is no stranger to its use. For example, artificial insemination involves the use of different plastic materials, including semen straws and insemination catheters, which increase the levels of waste in our environment. Finding a sustainable solution to avoid plastic materials is a challenge. The aim of this study was to examine different biodegradable materials as alternatives to conventional syringes used for refrigerated equine semen. The materials tested were bamboo, avocado, grass, paper, Kraft paper, wheat, and rice. Bamboo and avocado were selected to continue testing with the refrigerated semen with a lifespan extended by INRA 96®; the rest of the materials did not meet the requirements necessary for preservation. Motility and movement kinetics tests were performed at 24 and 96 h of refrigeration to evaluate semen quality; relative to the control, significantly better results were displayed in regard to the total and progressive motility avocado straws. The kinetic parameters were comparable to the control for both materials and within normal ranges. After performing a microbiological control process for both straws, it was confirmed that there was no bacterial growth in either straw. We can confirm that avocado straws can be used to preserve chilled equine semen, guaranteeing seminal quality while being respectful to the environment. [ABSTRACT FROM AUTHOR]

Simple Summary: This study was conducted to estimate the rate of Salmonella spp. isolation from cage papers, located in the bottom of canaries' cages and those of other exotic pet birds, in the 2015 Argentinean canary breeder championship. Furthermore, we determined the antimicrobial resistance profile of the isolates for antibiotics and commercial disinfectants. One pool of 258 cage paper pools was positive for Salmonella spp. (0.4%). Two strains of Salmonella serotype Glostrup were isolated, which were only resistant to sulfonamides and erythromycin and sensitive to alkali-based product PL301 AS. Although the rate of Salmonella spp. isolation from cage papers in an Argentinean canary breeder championship is low, this is the first study conducted in Argentina on Salmonella spp. isolation from these materials of pet birds. However, Salmonella ser. Glostrup isolated strains could be a source of human Salmonella outbreaks, and they show high resistance to disinfecting products. Birds, including canaries and other birds, have become increasingly popular as pets. Bird fairs, where breeders gather and show their production in a championship setting, present a setting for possible Salmonella spp. contamination and transmission. Therefore, this study estimated the rate of Salmonella spp. isolation from cage papers, located in the bottom of cages of exotic pet birds, including canaries. Collected Salmonella isolates were used to determine the antimicrobial resistance profile to 52 antibiotics and 17 commercial disinfectants, based on pure or a mixture of acids, alcohols, aldehydes, alkalis, halogens, peroxygen, and quaternary ammonium compounds. The samples consisted of 774 cage papers taken in the 2015 Argentinean canary breeder championship, pooling three cage papers into one sterile sampling bag. Only one pool of the cage papers was positive for Salmonella spp. (0.4%), which belonged to the sample from three frill canary cages. Two strains of Salmonella serotype Glostrup were isolated, which were only resistant to sulfonamides and erythromycin and sensitive to alkali-based product PL301 AS. Although the rate of Salmonella spp. isolation from cage papers in an Argentinean canary breeder championship is low, it should not be discounted because Salmonella ser. Glostrup can be a source of human Salmonella outbreaks and they show high resistance to disinfecting products. [ABSTRACT FROM AUTHOR]

Simple Summary: Paper mulberry (PM) is an unconventional protein feed material, and silage is its main processing method. The present study aimed to investigate the health benefits and meat quality of supplementing Yangzhou geese with paper mulberry silage. Results indicated that paper mulberry silage supplementation had a promotional effect on the growth of Yangzhou geese, the sensory quality of the breast muscles improved, and the nutritional quality enhanced. Untargeted metabolomics analysis demonstrated that PM treatment enhanced guanidinoacetic acid levels in breast muscles and facilitated the metabolism of amino acids and the role of substances in lipid antioxidant pathways. Consequently, paper mulberry could be considered to be a novel protein feed option capable for geese. There have been few investigations into the health benefits and meat quality of supplementing Yangzhou geese with paper mulberry silage. One hundred and twenty 28-day-old Yangzhou geese were selected for the experiment and randomly divided into two groups: a control group (CON) and a paper mulberry silage group (PM), with six replicates in each group. The experiment lasted for a total of 6 weeks. The experiment found that compared with CON, PM had a promoting effect on the average daily weight gain of Yangzhou geese (p = 0.056). Sensory and nutritional analysis of breast muscles revealed a decrease in a* value (p < 0.05) and an increase in protein content (p < 0.05) following PM treatment. Through untargeted metabolomics analysis of breast muscle samples, it was found that 11 different metabolites, including guanidinoacetic acid and other substances, had a positive effect on amino acid metabolism and lipid antioxidant pathways of PM treatment. Overall, the strategy of feeding Yangzhou geese with paper mulberry silage is feasible, which can improve the sensory quality and nutritional value of goose meat. The experiment provides basic data for the application form of goose breeding, so exploring the impact of substances within paper mulberry on goose meat should be focused on in the future. [ABSTRACT FROM AUTHOR]

Simple Summary: The article studies the research output on sheep and goats from Greece; a country where small ruminant farming is the most important terrestrial animal farming business. Milk production from sheep and goats exceeds that from cattle and over 90% of total milk produced from sheep and goats is used for dairy products. Research output on sheep and goats have increased significantly from 1997 to 2022. The bulk of publications, 87.5% of relevant papers, has originated from four establishments: Aristotle University of Thessaloniki; University of Thessaly; Agricultural University of Athens and Hellenic Agricultural Organization—Dimitra. Papers were published most frequently in the journals Small Ruminant Research and Journal of the Hellenic Veterinary Medical Society. The most frequent general topics of study were animal health-welfare and animal products. The findings have indicated that research has focused on milk production and diseases of the udder of small ruminants; moreover, there was accumulation of relevant research in Greece in some establishments only. The findings of the study can be used by researchers; stakeholders and Government entities to improve relevant research and to better allocate resources in the country. The study is a scientometrics evaluation of published articles performed in Greece on sheep and goats during the last 25 years, a period coinciding with implementation of reforms to shape and consolidate tertiary education and research establishments in the country. Objectives were: evaluation of the relevant publications and presentation of quantitative characteristics regarding scientific content and bibliometric details. The Web of Science platform was used (search terms: [[sheep OR goat*] OR [Ovis aries OR Capra hircus]] (1997–2022)) and 1080 papers were considered in detail. Throughout the study period, there was a clear progressive increase in numbers of papers published. The papers originated from 39 different entities, most from Aristotle University of Thessaloniki (34.0%), University of Thessaly (28.0%), Agricultural University of Athens (21.2%) and Hellenic Agricultural Organization—Dimitra (13.6%). Papers were published in 318 different journals. Journals with more published papers were Small Ruminant Research and Journal of the Hellenic Veterinary Medical Society. The most frequent general topic of study in the papers was health and welfare (46.7% of papers); second most frequent topic was animal products (18.6%). The papers have received 16,558 citations, i.e., οn average 15.4 citations per paper; the h-index was 56, the i10-index was 518 and the yearly citations per paper were 1.71. Papers on goats had higher impact than papers on sheep. There were 1711 individual authors, of which 728 were first or last authors. In total, 24 authors have each co-authored ≥2.5% of all papers; five authors were each first or last in that proportion of all papers. The findings have indicated that relevant research has focused on milk production and diseases of the udder of small ruminants; moreover, there was accumulation of relevant research in Greece in some establishments only. The findings of the study can be employed to initiate improved relevant research approaches in the country. [ABSTRACT FROM AUTHOR]

Simple Summary: The red imported fire ant (RIFA, Solenopsis invicta) is an exotic pest that can harm humans and animals, cause economic loss to agriculture, and damage ecosystems. In the present study, we devised a practical method to train detection dogs without introducing live RIFAs and an alternative way to correctly identify RIFA-invaded zones. Both live RIFA-experienced and inexperienced detection dogs successfully indicated RIFA-scented filter papers and live RIFAs with a high positive indication rate (>93%) and low false response rate (2%), and also performed successfully in field studies. In addition, the RIFA-scented filter papers can be stored at −20 °C and 4 °C at least 13 weeks for dog identification. Using filter paper as a RIFA odor bearer for detection dog training and RIFA identification is an effective and economical method in order to decrease the risk of RIFA introduction into uninvaded or eradicated areas. The red imported fire ant (RIFA, Solenopsis invicta) is an exotic aggressive pest that is notorious for its ability to seriously harm humans and animals, cause economic loss to agriculture, and damage ecosystems. This is the first study to validate the capability of filter paper adsorption as a feasible odor bearer of RIFAs and evaluate its use in detection dog training. Two live RIFA-experienced detection dogs achieved a mean 92% positive indication rate (PIR) on RIFA-scented papers with a relatively low false response rate (0.8%). The similar accuracies in recognizing live RIFAs (96%) and scented papers (92%) suggest that a filter paper is an effective odor reservoir. After training with live RIFA and scented filter papers, both RIFA-experienced and inexperienced detection dogs successfully indicated filter papers that were scented with at least 10 RIFAs for 4 h with a high PIR (>93%) and low false response rate (2%). Detection dogs correctly recognized the filter papers scented by 10 RIFAs for 24 h with a 97.6% PIR. Even for scented samples stored at −20 °C and 4 °C for 13 weeks, the positive indication rates (PIRs) were as high as 90%. These results suggest that filter paper is an effective RIFA odor bearer, and the scent can be maintained at least 13 weeks for dog identification. After RIFA-scented paper training, detection dogs showed high (>95%) PIRs for both RIFA-scented paper and live RIFAs and also successfully performed field studies. Using filter paper as a RIFA odor bearer is an effective and economical method for detection dog training and RIFA identification. [ABSTRACT FROM AUTHOR]

Simple Summary: With the growing popularity of snakes in the pet trade, investigation into environmental enrichment preferences in snakes is critical to their captive welfare. Unfortunately, small, minimalistic enclosures are predominantly used by both pet owners and breeders alike to house captive snakes. Recently, a handful of studies have shown the benefits that arise from the correct use of enrichment with climbing snakes, but none have observed this aspect in popular pet snakes that burrow, such as western hognose snakes. This study found that hognose snakes significantly prefer environmental enrichment when given a choice between enrichment and minimalistic conditions. This encourages further investigation into the welfare needs of other captive snake species that may be improperly housed, leading to chronic stress and poor health. The environmental enrichment needs of snakes are often disregarded. Using preference testing, we aimed to shed light on the enrichment preferences of a popular pet species, the western hognose snake (Heterodon nasicus). Snakes' enclosures were divided into enriched and standard sides. The enriched half had substrate for burrowing, interactive stimuli, and a large water dish. The standard half had paper towel substrate and a small water dish. Each side also contained a single shelter. We provided belly heat to create a thermal gradient on one side of the cage. Snakes were observed for 6 days, four times daily. We predicted a preference for enriched conditions and, as snakes are ectothermic, a preference for the warmer side. Snakes were additionally given an exploration assay, to explore whether differences in preference for environmental enrichment interact with boldness levels. We found that hognose snakes preferred enrichment, and the strength of this preference increased over time. Preference for enrichment was stronger when the enriched side was cooler. This may be due to the burrowing tendencies of these snakes. We found no relationship between preference and boldness. These findings emphasise the importance of preference testing in establishing research-informed enrichment opportunities for reptiles. [ABSTRACT FROM AUTHOR]

Simple Summary: People are increasingly concerned about the treatment and wellbeing of animals in zoos and aquariums, which has led to a big shift in how these places are seen by the public. For zoos and aquariums to keep up with these changing views, they need to understand how society thinks about them. Over the past 30 years, many studies have looked at how people feel about zoos and aquariums. This review seeks to compile recent research that offers insight into how contemporary societies view these establishments. One finding is that a higher number of female researchers have been involved in studies on zoos and aquariums compared to other areas of science, and previous studies have reported that women tended to be more empathetic towards animals and animal welfare than men. Most of the research has been conducted in Western countries, which creates a gap in our understanding from a global point of view. The studies also did not focus much on aquariums or children, and there is a need for more research that assesses changes in visitors' perceptions of animal wellbeing and the role that zoos and aquariums play in species conservation that compares attitudes before and after their visit. These insights highlight important areas for future research that could help shape how zoos and aquariums evolve and improve. Zoos and aquariums attract millions of visitors around the world. At the same time, the functions they fulfill and the way they do that have been subject to scrutiny from different fields of knowledge. The way societies perceive animals has evolved in recent decades and so has the social perception of institutions that work with them. This scoping review aims to gather studies from the last three decades that shade light on how current societies perceive zoos and aquariums. We selected papers describing quantitative studies published in English in peer-reviewed journals. We reviewed 71 works from different fields of knowledge. The proportion of female authors was noticeably higher than in general science. Most studies were carried out in culturally Western countries, a fact that constitutes a gap in knowledge from a global perspective. We also noticed an underrepresentation of aquariums and of children in the analyzed papers, and a need for more pre–post studies to ascertain the effect of a zoo or aquarium on its visitors. These conclusions point out interesting directions for future studies to help scientists, managers, and citizens design the future of these institutions. [ABSTRACT FROM AUTHOR]

Simple Summary: Considering that there are many ways to approach animal welfare, we aimed to study the value attributed to the animals themselves in scientific papers about farm animals published in animal welfare (AW) and animal production (AP) journals over time. All the papers were analyzed by five assessors, resulting in moderate agreement because the language used in the animal welfare scientific literature is ambiguous in relation to why and for whom it is performed. The overall mean score was 5.60, showing a low consideration of the interest of animals, close to neutrality. While AW journal scored higher and improved over the decades, the AP average score was below 5.0 and did not improve with time. The statement of the main justification for animal welfare papers, with an explicit declaration of their motivational priorities is important for the improvement of animal welfare science. There are, in the literature, distinct ways to approach animal welfare. The objective of this work was to study the value attributed to farm animals in the scientific papers published in animal welfare and animal production journals at three different points in time, separated by a decade each. The first ten papers mentioning "animal welfare" or "animal well-being" in their objectives or hypotheses from each journal and each focus year were selected. The 180 papers were blindly scored by five assessors between 1 and 10, according to the degree of intrinsic value attributed to animals. The overall mean score and standard deviation were 5.60 ± 2.49, with 6.46 ± 2.29 and 4.74 ± 2.40 for AW and AP journals, respectively, and 5.37 ± 2.44, 5.68 ± 2.52 and 5.75 ± 2.41 for the focus years of 2000, 2010 and 2020, respectively. There was an interaction between focus year and publication area: papers from AW journals scored better over time, in contrast with papers from AP journals, for which scores remained similar over decades. The inter-assessor agreement is moderate, which may reflect the subject complexity, as the language used in the papers studied was ambiguous in relation to why and for whom it is performed. The low overall mean score evidenced that the animal welfare scientific publications are, on average, not prioritizing the interests of the animals. Thus, our results evidenced the presence of animal welfarism in animal welfare science, a problem that seems not to be intrinsic to animal welfare science itself, but rather to the way research is frequently conceived, conducted, interpreted, summarized and applied. Therefore, it seems urgent to further study the motivation for animal welfare research. The statement of the main justification for animal welfare papers, with an explicit declaration of the motivational priorities that constitute each scientific animal welfare study, may be an interesting recommendation for the improvement of animal welfare science. [ABSTRACT FROM AUTHOR]

Simple Summary: In this study, several different types of nesting materials were tested: common reed and commercial structures made of wood, MDF, plastic, paper, or polystyrene. Red mason bees, Osmia bicornis L., most willingly nested in the reed and MDF forms (90% of the holes were occupied). However, they were reluctant to nest in the polystyrene (only 2% of the holes were occupied). MDF, wood, and paper materials turned out to have the lowest level of hygiene, because they flexed and leaked under the influence of moisture, which promoted pollen mold and the migration of Chaetodactylus osmiae mites along and through the nest holes. We found the highest mortality in the plastic material (2 larvae/hole) and the lowest in the reed (0.92 larvae/hole). The materials in which the bees' best reproductive results were obtained were the reed and plastic forms (despite the high level of larve mortality): the authors recommend these two types of materials for the commercial breeding of red mason bees. Osmia bicornis L. is a widespread and valued pollinator species. It is considered to be easy to breed, provided that the nesting material in which the bees build their nests is of sufficient quality and quantity. The aim of this study was to test several different types of nesting materials: reeds and commercial structures, including wood, MDF (Medium Density Fibreboard), plastic, paper or polystyrene. The highest levels of nest cavity occupancy were found in reeds (90%) and grooved MDF (over 80%). We have shown that maintaining mason bee colonies in polystyrene leads to reproductive losses (occupancy only 2% of nesting holes). Mason bees built the most cells in MDF (8.02 cells/hole) and wood (7.34 cells/hole), slightly fewer in plastic (6.83 cells/hole) and reeds (6.74 cells/hole), and the fewest in paper (3.67 cells/hole). The most cocoons per nest were obtained from reed (average 5.47), MDF (4.84) and plastic (4.74). We observed the highest mortality in plastic (2 larvae/hole), and the lowest in reeds (0.92 larvae/hole). In nests made of wood, MDF and paper, large nesting losses were caused by the migration of Ch. osmiae mites along and through the nest holes. The most hygienic nesting material turned out to be reed and plastic forms. [ABSTRACT FROM AUTHOR]

Simple Summary: This review seeks to understand how scientific research on trace elements in beef cattle has been developed and how the interaction of this theme with topics related to animal, environmental and human health has been established. Given the duality of many of the trace elements, being known as nutrients in small amounts or toxic when they exceed small concentrations, we brought the One Health perspective to analyse how researchers approach this research theme. In this work, we propose a path through scientific production to promote innovation, sustainability of animal production, food safety and human health. The objective was to investigate the context, approach and research topics present in the papers that analysed trace elements in beef cattle to identify gaps and scientific perspectives for the sustainable management of trace elements in livestock. The main research groups came from the United States, Spain, Japan, Brazil, India and Slovakia, which represented 31% of the papers produced. Only 37% of studies addressed aspects that integrated animal, environmental and human health. The reviewed papers concerned 56 elements and 15 bovine tissues (Cu, Zn, Pb, liver, muscle and kidney highlighted). The main gaps were (1) lack of research in developing countries, (2) the need to understand the impact of different environmental issues and their relationship to the conditions in which animals are raised, and (3) the need to understand the role of many trace elements in animal nutrition and their relationship to environmental and human health. Finally, we highlight possible ways to expand knowledge and provide innovations for broad emerging issues, primarily through expanding collaborative research networks. In this context, we suggest the adoption of the One Health approach for planning further research on trace elements in livestock. Moreover, the One Health approach should also be considered for managers and politicians for a sustainable environmental care and food safety. [ABSTRACT FROM AUTHOR]

Simple Summary: Equids, such as horses, donkeys, and mules, play vital roles in various types of work globally, with their use differing significantly across countries of different income levels. We conducted a thorough review of research literature to examine if and how terms related to working equids are used in high-, middle-, and low-income countries. Our findings indicate that studies focusing on higher-income countries tend to produce more research papers on equids, except for when using the terms "working equid" and "draft equid", which are more common in studies focusing on lower-income countries, and these terms often refer to animals in low-resource rural settings and marginalised communities. We suggest that "working equid" should be defined as "any equid engaged in physical labour that provides a significant or direct contribution to the economic livelihood, sustenance or support of the owner/user's family, typically within a low resource setting". The lack of a standard definition complicates policy making and welfare legislation, especially in lower-income countries where equids are crucial for livelihoods but are often overlooked by policy. This study highlights the need for more inclusive and culturally aware terminology to improve welfare standards and policies for working equids worldwide. Equids are engaged in myriad types of work across the world, with contexts and industries varying widely between high-, middle- and low-income countries as classified by the World Bank. Through a comprehensive abstract search and literature review, we examined the usage and context of terms associated with working equids in high-income countries (HICs), upper-middle-income countries (UMICs), lower-middle-income countries (LMICs), and low-income countries (LICs). Analysis showed that the search term used was significantly associated with World Bank country income classification. All search terms except two returned a significantly higher number of papers for higher-income countries compared to lower-income countries. The two exceptions were "working equid" and "draft equid", which returned significantly more papers for lower-income countries than higher-income countries. Both terms also returned papers from high-income and upper-middle-income countries, but these were always in the context of low-resource settings and rural or marginalised communities, of which we provide examples in the discussion. We propose that the term "working equid" should be used to describe "any equid engaged in physical labour that provides a significant or direct contribution to the economic livelihood, sustenance or support of the owner/user's family, typically within a low resource setting". Our review highlights the intricate interplay between socioeconomic factors and examines how equids are described in the literature. The lack of a universally accepted definition leads to challenges in policy formulation, resource allocation, and welfare standards development, particularly in LMICs and LICs where working equids are crucial to livelihoods. This study underscores the need for a more inclusive and comprehensive approach to terminology, advocating for future research to bridge linguistic and cultural gaps in understanding working equids globally. Such efforts are vital for equitable and effective welfare standards and policy development for these animals. [ABSTRACT FROM AUTHOR]

This document is a summary of a special issue of the journal Animals, which focuses on the welfare, ethics, and behavior of animals in zoos and aquariums. The authors emphasize the importance of continually improving animal welfare in these settings through transparent and scientifically rigorous research. They acknowledge the diversity of philosophical views on keeping animals in captivity and highlight the role of zoos and aquariums in conservation efforts. The papers in this issue cover a range of topics, including the ethics and legal concerns of zoo and aquarium species, strategies for improving animal welfare, and the need to critically evaluate data on controversial topics. The authors stress the need for ongoing dialogue and collaboration to advance animal welfare in these settings. [Extracted from the article]

Simple Summary: The development of data recording standards is important for the modern dairy industry. This paper summarizes a series of discussions from dairy industry experts. During the meetings, researchers, industry representatives, and farmers discussed current challenges and proposed solutions for establishing data standards in the dairy industry. It was highlighted that both on and off-farm factors make data standardization difficult in the dairy industry. Hence, it was suggested that a collaborative approach between academia, industry, and the government is needed for the successful establishment of data recording standards in the dairy industry. Standards for data generation and collection are important for integration and for achieving data-driven actionable insights in dairy farming. Data integration and analysis are critical for advancing the dairy industry, enabling better decision-making, and improving operational efficiencies. This commentary paper discusses the challenges of and proposes pathways for standardizing data generation and collection based on insights from a multidisciplinary group of stakeholders. Drawing from a series of meetings of industry experts, academics, and farmers organized under the Dairy Brain Project's Coordinated Innovation Network (CIN), we explore the benefits of creating uniform data generation and collection protocols to ensure compatibility and reliability across different data sources. Key insights include the importance of defining standardization at both farm and industry levels, the role of education and incentives, and the potential for using existing frameworks such as the International Committee for Animal Recording. Additionally, we highlight industry-specific case studies, including successful examples from Brazil such as GERAR, which focuses on reproductive performance data, and Labor Rural, which integrates data from multiple farms to provide valuable insights to farmers and milk processors. The paper concludes with recommendations for implementing these protocols and highlights the need to foster collaboration among stakeholders for the successful implementation and adoption of standardized data generation and collection protocols in the dairy industry. [ABSTRACT FROM AUTHOR]

Simple Summary: As global pet ownership rises, managing the health of aging dogs has become a growing concern, particularly due to the high costs and challenges in recognizing early symptoms. In Korea, many casual pet owners struggle to identify abnormal behaviors and address them effectively without expert knowledge. Thus, we propose a pre-diagnosis system for casual dog owners that infers possible diseases from queries based on observed abnormal symptoms. This proposed system, based on a fuzzy inference algorithm, is not to aiming to compete with veterinarians but to alert the casual pet caregiver to monitor abnormal behavior indicative of disease, to be addressed as early as possible. The experimental results verify that the proposed system is sufficiently robust under noisy input, which is very common when non-expert casual owners use this system. While the pet market is continuously rapidly increasing in Korea, pet dog owners feel uncomfortable in coping with pet dog's health problems in time. In this paper, we propose a pre-diagnosis system based on neuro-fuzzy learning, enabling non-expert users to monitor their pets' health by inputting observed symptoms. To develop such a system, we form a disease–symptom database based on several textbooks with veterinarians' guidance and filtering. The system offers likely disease predictions and recommended coping strategies based on fuzzy inference. We evaluated three fuzzy inference algorithms—PFCM-R, FHAL, and MNFL. While PFCM-R achieved high accuracy with clean data, it struggled with noisy inputs. FHAL showed better noise tolerance but lower precision. PFCM-R is a variant of well-known fuzzy unsupervised learner FCM, and FHAL is the hybrid fuzzy inference engine based on Fuzzy Association Memory and a double-layered FCM we developed. To make the system more robust, we propose the multi-layered neuro-fuzzy learner (MNFL) in this paper, which effectively weakens the association strength between the disease and the observed symptoms, less related to the body part on which the abnormal symptoms are observed. In experiments that are designed to examine how the inference system reacts under increasing noisy input from the user, MNFL achieved 98% accuracy even with non-erroneous inputs, demonstrating superior robustness to other inference engines. This system empowers pet owners to detect health issues early, improving the quality of care and fostering more informed interactions with veterinarians, ultimately enhancing the well-being of companion animals. [ABSTRACT FROM AUTHOR]

Simple Summary: Different reproductive biotechnologies have been applied to sheep, such as cloning, which has been successfully applied in this species. In this context, the aim of the present study was to carry out a bibliometric analysis of the scientific literature on cloning applied to sheep reproduction, since the first report was published, to identify the most cited articles, main authors and collaboration among them, published journals, institutions with more published papers, most prolific countries and the network collaboration among them, and research topics. This study collected bibliographic data from 124 papers relating to cloning of sheep. The articles that were cited more often addressed topics related to the generation of transgenic animals, recovery of wild species, and xenotransplants. So far, no bibliometric studies have been conducted about cloning of sheep. Somatic cell nuclear transfer (SCNT) is a reproductive biotechnology with great potential in the reproduction of different species of zootechnical interest, including sheep. This study aimed to carry out a bibliometric analysis of scientific papers published on the application of SCNT in sheep reproduction during the period 1997–2023. The search involved the Science Citation Index Expanded and Social Sciences Citation Index databases of the main collection of the Web of Sciences with different descriptors. A total of 124 scientific papers were analyzed for different bibliometric indicators using the VOSviewer software. Since 2001, the number of SCNT-related papers that have been published concerning sheep reproduction has increased and it has fluctuated in ensuing years. The main authors, research groups, institutions, countries, papers, and journals with the highest number of papers related to the application of SCNT in sheep reproduction were identified, as well as the topics that address the research papers according to the terms: somatic cell, embryo, oocyte, gene expression, SCNT, and sheep. [ABSTRACT FROM AUTHOR]

Simple Summary: The quality of muscle plays an important role in improving the economic benefit of aquatic products. The paper mulberry (Broussonetia papyrifera, BP) grows wildly in the south of Asia. In this experiment, grass carps (initial weight: 50.0 ± 0.5 g) were fed diets with the addition of 0%, 5%, 10%, 15% and 20% BP in a control diet (crude protein: 31%, crude fat: 3.8%) for 8 weeks. In conclusion, adding 5% BP did not affect the growth of grass carp. However, the supplementation of 10% BP could improve muscle quality through improving muscle hardness, reducing fat accumulation and muscle fiber diameter, at the cost of reducing growth performance. The present study investigated the effects of dietary paper mulberry (Broussonetia Papyrifera, BP) on growth performance, muscle quality and muscle growth-related mRNA expressions of grass carp. Fish (initial weight: 50.0 ± 0.5 g) were fed diets supplemented with 0% (control diet), 5%, 10%, 15% and 20% BP for 8 weeks. The results showed that increasing levels of paper mulberry linearly and quadratically decreased the special gain rate (SGR) and increased the feed conversion rate (FCR) of grass carp (p < 0.05). Significantly positive quadratic trends were found between paper mulberry levels and muscle crude fat or crude protein of grass carp (p < 0.05). In comparison to the control diet, the 10%BP and 15%BP groups had significantly decreased muscle crude fat and increased crude protein (p < 0.05). The levels of paper mulberry resulted in a linear and quadratic increase in water loss of grass carp muscle (p < 0.05), and all groups with paper mulberry supplementation were significantly higher than the control group (p < 0.05). Significant positive linear and quadratic trends were found between the paper mulberry levels and muscle fiber diameter or density of grass carp (p < 0.05). In comparison to the control diet, the significant differences were found in the 15%BP and 20%BP groups (p < 0.05). The muscle adhesiveness and hardness linearly and quadratically increased with the increasing levels of paper mulberry (p < 0.05), and both of which increased significantly when the level of paper mulberry reached 10% (p < 0.05). In addition, the increase in paper mulberry linearly and quadratically improved the expressions of myoblast determination protein (MyoD), myogenin (MyoG), paired box protein 7 (Pax7) and myostatin 1 (MSTN1) (p < 0.05). When the supplementation of paper mulberry reached 15%, the expressions of all these mRNAs were significantly higher than those of the control group (p < 0.05). In summary, adding 5% paper mulberry did not affect the growth of grass carp. However, the supplementation of 10% paper mulberry could improve muscle quality through improving muscle hardness, reducing fat accumulation and muscle fiber diameter, at the cost of reducing growth performance. [ABSTRACT FROM AUTHOR]

Simple Summary: The usage of alfalfa (Medicago sativa L.) as a dietary protein source for ruminants in China is limited by forage quality and planting scale. Paper mulberry (Broussonetia papyrifera L., RY) has emerged as a new and representative high-protein woody forage resource for ruminants. However, information is less available regarding how substituting RY for alfalfa affect the fermentation and protein quality in total mixed ration (TMR) silages. This study evaluated ensiling characteristics, protein quality, and in vitro digestibility in TMR silages by mixing RY with alfalfa at different rations. The TMR were made with alfalfa and RY mixtures (36.0%), maize meal (35.0%), oat grass (10.0%), soybean meal (7.5%), brewers' grain (5.0%), wheat bran (5.0%), premix (1.0%), and salt (0.5%) on a dry matter basis, respectively. The alfalfa and RY mixtures were made in the following ratios of dry matter: 36:0 (RY0), 27:9 (RY9), 18:18 (RY18), 9:27 (RY27), and 0:36 (RY36). The results showed that RY substitution had no adverse effect on fermentation quality and nutritional composition, but inhibited true protein degradation, while decreasing in vitro dry matter and crude protein digestibility. Therefore, RY and alfalfa mixtures at a ratio of 18:18 is suitable for silage-based TMR. To develop an alternative high-protein forage resource to alleviate ruminant feed shortages, we investigated the effects of replacing alfalfa (Medicago sativa L.) with different ratios of paper mulberry (Broussonetia papyrifera L., RY) on fermentation quality, protein degradation, and in vitro digestibility of total mixed ration (TMR) silage. The TMR were made with alfalfa and RY mixtures (36.0%), maize meal (35.0%), oat grass (10.0%), soybean meal (7.5%), brewers' grain (5.0%), wheat bran (5.0%), premix (1.0%), and salt (0.5%) on a dry matter basis, respectively. The alfalfa and RY mixtures were made in the following ratios of dry matter: 36:0 (RY0), 27:9 (RY9), 18:18 (RY18), 9:27 (RY27), and 0:36 (RY36). After ensiling for 7, 14, 28, and 56 days, fermentation quality, protein degradation, and microbial counts were examined, and chemical composition and in vitro digestibility were analyzed after 56 days of ensiling. All TMR silages, irrespective of the substitution level of RY, were well preserved with low pH and ammonia nitrogen content, high lactic acid content, and undetectable butyric acid. After ensiling, the condensed tannin content for RY18 silages was higher than the control, but non-protein nitrogen, peptide nitrogen, and free amino acid nitrogen contents was lower, while the fraction B1 (buffer-soluble protein) was not different among all the silages. Dry matter and crude protein digestibility for RY27 and RY36 silages was lower than the control, but there was no difference between control and RY18 silages. This study suggested that ensiling RY with alfalfa inhibited true protein degradation, but decreased in vitro dry matter and crude protein digestibility of TMR silages, and that 18:18 is the optimal ratio. [ABSTRACT FROM AUTHOR]

Simple Summary: The present work presents a snapshot of the state of the art of Equine-Assisted Intervention (EAI) research, including published articles and the most cited research as well as the most used keywords, and prolific authors and co-authors and their countries. To our knowledge, no other bibliometric studies have been performed, so it can be a tool to support future research. Equine Assisted Interventions (EAIs) integrate the active participation of horses in therapeutic or educational interventions. A bibliometric analysis was carried out on this topic, using traditional bibliometric laws and recommendations. For this purpose, a search on the Web of Science (WoS) Core Collection database was carried out, obtaining 333 documents. Annual publications followed an exponentially increasing trend (R2 = 86%), pointing out that this topic is a growing interest among researchers, publishers, and journals. The USA was the most productive country worldwide and Jeong-yi Kwon and Ji Lee were the prolific co-authors. The WoS category with the highest number of papers was Rehabilitation (84 papers). The Journal of Alternative and Complementary Medicine, and Pediatric Physical Therapy were the journals with the highest number of publications. The most cited paper was "State of the Evidence Traffic Lights 2019: Systematic Review of Interventions for Preventing and Treating Children with Cerebral Palsy". The most used author keywords were rehabilitation, balance, and those related to specific populations such as Cerebral Palsy and Autism Spectrum Disorder. These results suggest that EAIs is a topic of increasing interest for researchers, editors, and professionals. [ABSTRACT FROM AUTHOR]

Simple Summary: Paper mulberry (Broussonetia papyrifera, PM) is a potential roughage source widely distributed in Asia, but the chemical composition, silage fermentation, and digestibility are not fully understood. Here, we compared the chemical composition, silage fermentation, and digestibility of leaf, stem, and whole plant of PM to evaluate its feeding value. The result showed that the leaf had lower fiber content and higher protein content than the stem and whole plant. Meanwhile, the stem silage had the lowest pH value and lactate content, while those in the leaf were the highest. The in vitro and in situ digestibility showed the leaf was more digestible. Our study gives the reference of different parts of PM to be used as a feedstuff. Paper mulberry (Broussonetia papyrifera, PM) is high protein but unutilized as a feed source. The study explores the different parts (leaf, stem, and whole plant) of PM chemical composition, silage fermentation, and in vitro and in situ digestibility, aiming to give some guidelines to PM usage as feed. The result showed that the leaf had a higher fresh weight than the stem (p < 0.05). The dry matter contents of the three groups had no differences. The highest crude protein, ether extract, water-soluble carbohydrate, ash, calcium, phosphorus, amino acid contents, and butter capacity were observed in the leaf (p < 0.05). The stem had the highest (p < 0.05) neutral detergent fiber, acid detergent fiber, and lignin contents. After ensiling, the stem silage had the lowest pH value, ammonia nitrate (NH3-N), lactate, acetate, and propionate (p < 0.05). The leaf silage had the highest pH value (p < 0.05). The lactate, acetate, and propionate in the leaf and whole plant silage had no difference. The butyrate was not detected in all silage. The in vitro and in situ digestibility experiments showed the leaf had the highest digestibility (p < 0.05), which could produce more volatile fatty acids and have a higher effective digestibility. These results allow a greater understanding of PM to be used as a feedstuff. [ABSTRACT FROM AUTHOR]

Simple Summary: Through gesture recognition and detection of sika deer, farmers can observe the gestures of sika deer without physical contact, providing data and technical support for the intelligent and welfare-oriented breeding of sika deer. This study is based on the YOLOv8 network model. By optimizing the convolution module, incorporating the attention mechanism, and enhancing the detection head module, a new method for detecting sika deer poses was developed. The method was assessed using four behavioral datasets, which included standing, lying, eating, and attacking. The pose-recognition accuracy of sika deer significantly improved to an average of 91.6%, laying a foundation for the health assessment and information management of sika deer. As the sika deer breeding industry flourishes on a large scale, accurately assessing the health of these animals is of paramount importance. Implementing posture recognition through target detection serves as a vital method for monitoring the well-being of sika deer. This approach allows for a more nuanced understanding of their physical condition, ensuring the industry can maintain high standards of animal welfare and productivity. In order to achieve remote monitoring of sika deer without interfering with the natural behavior of the animals, and to enhance animal welfare, this paper proposes a sika deer individual posture recognition detection algorithm GFI-YOLOv8 based on YOLOv8. Firstly, this paper proposes to add the iAFF iterative attention feature fusion module to the C2f of the backbone network module, replace the original SPPF module with AIFI module, and use the attention mechanism to adjust the feature channel adaptively. This aims to enhance granularity, improve the model's recognition, and enhance understanding of sika deer behavior in complex scenes. Secondly, a novel convolutional neural network module is introduced to improve the efficiency and accuracy of feature extraction, while preserving the model's depth and diversity. In addition, a new attention mechanism module is proposed to expand the receptive field and simplify the model. Furthermore, a new pyramid network and an optimized detection head module are presented to improve the recognition and interpretation of sika deer postures in intricate environments. The experimental results demonstrate that the model achieves 91.6% accuracy in recognizing the posture of sika deer, with a 6% improvement in accuracy and a 4.6% increase in mAP50 compared to YOLOv8n. Compared to other models in the YOLO series, such as YOLOv5n, YOLOv7-tiny, YOLOv8n, YOLOv8s, YOLOv9, and YOLOv10, this model exhibits higher accuracy, and improved mAP50 and mAP50-95 values. The overall performance is commendable, meeting the requirements for accurate and rapid identification of the posture of sika deer. This model proves beneficial for the precise and real-time monitoring of sika deer posture in complex breeding environments and under all-weather conditions. [ABSTRACT FROM AUTHOR]

Simple Summary: Through the non-destructive recognition and tracking algorithm of multi-objective behaviors of beef cattle, practitioners are able to obtain beef cattle feeding information in an all-round way, which lays the foundation for intelligent farming. This study is based on the deep learning recognition algorithm YOLOv8 and tracking algorithm Deep SORT. Through optimizing the convolution module, introducing the attention mechanism, improving the re-identification network, and the trajectory generation and matching process, a new method for the non-destructive identification and tracking of multi-target behaviors of beef cattle is constructed. The average accuracy of nine behaviors can be up to 96.5%, and the accuracy of multi-target tracking is up to 92.1%, which can provide technical support for beef cattle management. The method proposed in this paper provides theoretical and practical support for the intelligent recognition and management of beef cattle. Accurate identification and tracking of beef cattle behaviors are essential components of beef cattle production management. Traditional beef cattle identification and tracking methods are time-consuming and labor-intensive, which hinders precise cattle farming. This paper utilizes deep learning algorithms to achieve the identification and tracking of multi-object behaviors in beef cattle, as follows: (1) The beef cattle behavior detection module is based on the YOLOv8n algorithm. Initially, a dynamic snake convolution module is introduced to enhance the ability to extract key features of beef cattle behaviors and expand the model's receptive field. Subsequently, the BiFormer attention mechanism is incorporated to integrate high-level and low-level feature information, dynamically and sparsely learning the behavioral features of beef cattle. The improved YOLOv8n_BiF_DSC algorithm achieves an identification accuracy of 93.6% for nine behaviors, including standing, lying, mounting, fighting, licking, eating, drinking, working, and searching, with average 50 and 50:95 precisions of 96.5% and 71.5%, showing an improvement of 5.3%, 5.2%, and 7.1% over the original YOLOv8n. (2) The beef cattle multi-object tracking module is based on the Deep SORT algorithm. Initially, the detector is replaced with YOLOv8n_BiF_DSC to enhance detection accuracy. Subsequently, the re-identification network model is switched to ResNet18 to enhance the tracking algorithm's capability to gather appearance information. Finally, the trajectory generation and matching process of the Deep SORT algorithm is optimized with secondary IOU matching to reduce ID mismatching errors during tracking. Experimentation with five different complexity levels of test video sequences shows improvements in IDF1, IDS, MOTA, and MOTP, among other metrics, with IDS reduced by 65.8% and MOTA increased by 2%. These enhancements address issues of tracking omission and misidentification in sparse and long-range dense environments, thereby facilitating better tracking of group-raised beef cattle and laying a foundation for intelligent detection and tracking in beef cattle farming. [ABSTRACT FROM AUTHOR]

Simple Summary: Paper mulberry (Broussonetia papyrifera; PM) is a type of roughage rich in bioactive substances, such as phenolics and flavonoids, which are beneficial for animal health. This study evaluated the apparent digestibility of PM silage in Holstein dairy cows and its effect on the milk production, antioxidant capacity, and fecal bacteria composition of the animals. The results showed that the PM silage had no significant influence on the milk yield, apparent digestibility, and fecal bacteria composition of dairy cows. However, diets with PM silage can enhance the antioxidant and immune capacity of dairy cows, mainly due to the bioactive substance in PM. Today, faced with a shortage of feedstuff resources in ruminants, PM can be a useful feed resource for ruminants. Simultaneously, with the ban on antibiotics, PM may become an important functional feed for protecting animal health. Paper mulberry (Broussonetia papyrifera; PM) is an excellent and extensive type of roughage in Asia. This study aimed to evaluate the effects of PM silage on the milk production, apparent digestibility, antioxidant capacity, and fecal bacteria composition in Holstein dairy cows. Forty-five lactating Holstein dairy cows with a similar milk yield and parity were selected and randomly assigned to three groups. The control group was fed a non-PM silage diet, and the PM-treated groups were fed 4.5 and 9.0% PM silage supplementary diets for 28 days. Then, treatment groups were fed diets containing 13.5 and 18.0% PM silage for the next 28 days, respectively. PM silage increased the milk urea nitrogen and decreased the somatic cell count (p < 0.05), but did not affect the dry matter intake, milk yield, apparent digestibility, and energy balance of dairy cows. PM silage can enhance the blood total antioxidant capacity, superoxide dismutase, and immune globulin content (p < 0.05). The PM silage significantly decreased the relative abundance of the genera Ruminococcaceae UCG-013 and Tyzzerella-4 (p < 0.05). In conclusion, PM silage enhanced the antioxidant capacity and immunity of dairy cows, but did not influence the milk yield, dry matter digestibility, and fecal bacteria composition. [ABSTRACT FROM AUTHOR]

The document "Lameness Is Not an Excuse Anymore" discusses the importance of addressing lameness in dairy cattle, which is a significant health and welfare issue affecting more than fifty percent of animals in Western Europe. The text emphasizes the need for better understanding, treatment, and prevention of lameness, as well as the economic impact it has on dairy herds. The document also highlights the research efforts from various countries to study and address lameness in dairy cows, providing practical information for veterinary practitioners to improve foot care and treatment practices. [Extracted from the article]

Simple Summary: As agriculture and human settlements expand, conflicts between humans and animals become more frequent, resulting in resource loss and safety risks. Therefore, accurately identifying and locating offending animals is essential. This study presents a novel method for automatically detecting offending animals, especially in situations where they are obscured or the images are unclear. This research focused on six types of offending animals commonly found in northeastern China. By employing improved image processing techniques, this study enhanced detection accuracy in complex environments. Experimental results demonstrate that the proposed method outperforms existing techniques on the dataset used. This new approach helps improve the accuracy of intelligent monitoring systems, providing better technical support to minimize conflicts between humans and animals, thus protecting agriculture and ensuring the safety of both humans and animals. Conflicts between humans and animals in agricultural and settlement areas have recently increased, resulting in significant resource loss and risks to human and animal lives. This growing issue presents a global challenge. This paper addresses the detection and identification of offending animals, particularly in obscured or blurry nighttime images. This article introduces Multi-Channel Coordinated Attention and Multi-Dimension Feature Aggregation (MDA-DETR). It integrates multi-scale features for enhanced detection accuracy, employing a Multi-Channel Coordinated Attention (MCCA) mechanism to incorporate location, semantic, and long-range dependency information and a Multi-Dimension Feature Aggregation Module (DFAM) for cross-scale feature aggregation. Additionally, the VariFocal Loss function is utilized to assign pixel weights, enhancing detail focus and maintaining accuracy. In the dataset section, this article uses a dataset from the Northeast China Tiger and Leopard National Park, which includes images of six common offending animal species. In the comprehensive experiments on the dataset, the m A P 50 index of MDA-DETR was 1.3%, 0.6%, 0.3%, 3%, 1.1%, and 0.5% higher than RT-DETR-r18, yolov8n, yolov9-C, DETR, Deformable-detr, and DCA-yolov8, respectively, indicating that MDA-DETR is superior to other advanced methods. [ABSTRACT FROM AUTHOR]

Simple Summary: Mesenchymal stromal cells are used for their regenerative properties both in human and veterinary medicine. Usually, these cells are collected from bone marrow or adipose tissue, but methods that allow a simple, economical, and non-invasive collection of cells would encourage their use. One easily accessible alternative source would be urine. In 2008, urine mesenchymal stromal cells were isolated for the first time from human urine. These cells come from the kidney and would, therefore, be ideal for use in the treatment of kidney/genitourinary diseases. In this paper, urine mesenchymal stromal cells were isolated from healthy dogs and dogs affected by chronic kidney disease. Three collection methods (spontaneous micturition, bladder catheterization, and cystocentesis) were compared and cells were analyzed for their mesenchymal properties. The isolated cells met the criteria set by the international society of stem cell therapy to be defined as stem cells. Cells isolated from sick dogs proliferated more readily than those isolated from healthy dogs and may provide a therapeutic option for the treatment of chronic kidney disease. However, in this study, cells could only be isolated from urine samples collected through cystocentesis. Urine-derived mesenchymal stromal/stem cells (USCs) could be a valuable source of cells in regenerative medicine because urine can be easily collected non-invasively. In this paper, USCs were isolated from both healthy dogs and dogs affected by chronic kidney disease (CKD), and the efficacy of collection methods (spontaneous micturition, bladder catheterization, and cystocentesis) were compared. Isolated cells were cultured in the presence of platelet-rich plasma and studied for their proliferative capacity (growth curve, doubling time, and colony forming unit), differentiation properties, expression of mesenchymal markers, and Klotho protein. Morphologically, all cells were elongated and fibroblast-like. USCs isolated from samples collected by spontaneous micturition and bladder catheterization failed to proliferate, whilst USCs obtained by cystocentesis showed a doubling time of 2.04 days in healthy dogs and 1.7 days in dogs with CKD (p < 0.05). Cells were able to differentiate into osteogenic, chondrogenic, and adipogenic lines, showed positive expression to mesenchymal/stem markers, negative expression to hematopoietic markers, and major histocompatibility complex (MHCII) antigen. Klotho protein expression was confirmed. This study confirmed that USCs from healthy and CKD dogs can act as stem cells, with those from sick dogs having greater proliferative ability with the potential for use as autologous therapies. [ABSTRACT FROM AUTHOR]

The Special Issue "Behaviour, Applied Ethology and Welfare of Farmed Animals" explores empirical data and theoretical discussions on contemporary issues in animal welfare, focusing on the use of behavior as an indicator. The papers cover topics such as environmental enrichment, virtual fencing, dietary adjustments, and innovations to improve animal health and welfare in organic systems. The studies emphasize the importance of understanding, assessing, and improving animal welfare through applied ethology, highlighting the role of behavior in enhancing welfare conditions for farmed and captive animals. The articles also discuss the complexities of aligning animal welfare with economic realities and the need for policies that cater to different farm sizes while addressing the balance between welfare and economic constraints. [Extracted from the article]

Simple Summary: The present paper summarizes the detailed gross morphological and imagistic features of the pelvic skeletal elements alongside some radiographical interpretations. The added metrical data makes the anatomical description of the pelvic skeleton in Chlorocebus sabaeus more accurate and precise concerning these monkeys' anatomy. Several morphological features of each of the studied skeletal elements explain the different adaptative changes in this species as well, as it shows, in a comparative way, different sets of elements in the primate skeletal anatomy. The paper presents a detailed gross anatomical description of the elements of the pelvic limb in the African green monkey and provides comparative and differential elements on pelvic limb monkey osteology. The osteometric investigation adds value to the gross morphological and radiological investigation, adjoining metric data to the gross descriptive data set. The main methodology used was the gross morphological investigation, doubled by regular osteometrical and radiographical assessments. For each of the elements, several morphological aspects are described, pointing to the specific and differential aspects that might serve as landmarks in the specific diagnosis of different adaptative changes, related to the type of locomotion. For the pelvis, the aspect of the symphysis, the overall shape of the pelvic inlet, the iliac wing aspect and the acetabular shape are listed as significant elements. The femur has a spherical head with an inferior position of the femoral fovea and a well-developed (tall) greater trochanter, lacking the third trochanter. The medial condyle of the femur is better developed than the lateral one and an evident extensor's fossa is noted at the level of the distal shaft. The proximal lateral condyloid surface in the tibia is slightly larger than the medial one, with noticeable intercondilar eminences. The fibula is long and unfused with the tibia. Some specific anatomical features are listed only for the talus and calcaneus from tarsals. The study revealed the presence of the gastrocnemius sesamoid bones and the proximal sesamoidi bones as well. Investigation presents a series of osteometrical data with an attempt to show some basic metrical differences among the investigated specimens. [ABSTRACT FROM AUTHOR]

Simple Summary: This paper summarizes the experiences and perceptions of European equestrian educational experts from a workshop at the French National Riding School in Saumur. The workshop addressed key challenges in equestrian education, focusing on topics such as the horse–human relationship, diversity of perspectives, training philosophy, and social acceptability. The findings highlight the need for a cultural shift towards an evidence-based approach to training, with equine welfare at its core. Key challenges, including anthropomorphism and varying standards, threaten the social license to operate. The study suggests that educational institutions can lead this change, with further research needed on pedagogical challenges. This paper highlights and summarizes the experiences and perceptions of European equestrian educational experts from a workshop held at the French National Riding School in Saumur. The workshop, which brought together fifty leading experts, aimed to address challenges in equestrian education, identify common ground, and share best practices. Using 'The World Café' method, the participants were divided into groups to discuss four key topics: the relationship between horses and humans, diversity of perspectives versus common ground, training philosophy, and social acceptability. A follow-up online focus group was conducted six months later, and the discussions were transcribed and analyzed using qualitative content analysis. The results revealed the need for a universal cultural shift towards an evidence-based approach to riding and training horses, with equine welfare at its core. Key challenges, such as anthropomorphism, varying standards across disciplines and countries, and the evolving horse–human relationship, pose risks to the social license to operate. The findings suggest that educational institutions, with their shared values, are well-positioned to lead this change, although further research is needed to address pedagogical challenges. The study also emphasizes the importance of communicating 'good news stories' to promote evidence-based practices in training and competition. [ABSTRACT FROM AUTHOR]

Simple Summary: In the 21st century, more people than ever have a positive relationship with an individual bird or a family of birds, be this as a companion or a regular backyard visitor. People are willing to support birds in the wild, rehabilitate injured birds, and fight against the destruction of their habitat. However, our relationship with birds is double-edged. Each year billions of birds are killed. These are the cases of direct mortality caused via human actions and technology. Direct mortality plays a different and even more surprising role in the decline of avian species than does habitat loss or climate change. Direct mortality indiscriminately affects young and old, inexperienced and resourceful birds alike. This paper first confirms why we need birds in our ecosystems, and then it outlines all major causes of direct mortality. Finally, this paper evaluates how such unsustainable death rates can be reduced by altering the circumstances that cause high death rates in the first place. This may need adjustments to existing technologies or products, and even new legal frameworks. This paper presents a series of targeted strategies that have been or could be implemented nationally and internationally to avoid much of the human-caused direct mortality in birds. Human interaction with birds has never been more positive and supported by so many private citizens and professional groups. However, direct mortality of birds from anthropogenic causes has increased and has led to significant annual losses of birds. We know of the crucial impact of habitat loss on the survival of birds and its effects on biodiversity. Direct mortality via anthropogenic causes is an additive but biologically important cause of avian decline. This is the focus of this paper. This paper synthesises and interprets the data on direct anthropogenic causes of mortality in birds, and it also discusses emerging and relatively hidden problems, including new challenges that birds may not be able to manage. This paper points out that such deaths occur indiscriminately and have negative behavioural and reproductive consequences even for survivors. All of these factors are important to address, because any functional habitat depends on birds. This paper suggests that some of this death toll can be reduced substantially and immediately, even some of the seemingly intractable problems. This paper also proposes cross-disciplinary solutions, bearing in mind that "ecosystem services" provided by birds benefit us all, and that the continued existence of avian diversity is one cornerstone for human survival. [ABSTRACT FROM AUTHOR]

Simple Summary: As global demand for beef rises, cattle farmers face growing pressure to reduce costs, manage resources wisely, and maintain environmentally friendly practices. Feed efficiency, or the ability of cattle to gain weight while consuming less feed, has become a key solution to these challenges, as feed represents a major production cost. However, difficulties in measuring feed intake and addressing maintenance, reproduction, and productivity limit feed efficiency improvements for sustainable beef production. This paper reviews advancements in measuring and improving feed efficiency in beef cattle, from technologies that track how much individual animals eat to breeding methods that identify cattle with better genetic potential for feed efficiency. Using tools such as genomic data, scientists and producers can now predict which cattle will grow well on less feed, reducing the resources needed and lowering costs. Nutritional models are also helping producers optimize feeding strategies based on cattle needs, improving both efficiency and animal health. Combining these approaches, this review paper offers a roadmap for a more profitable and sustainable beef industry that can meet future demands without exhausting natural resources, and therefore benefiting producers and consumers. Increasing feed efficiency in beef cattle is critical for meeting the growing global demand for beef while managing rising feed costs and environmental impacts. Challenges in recording feed intake and combining genomic and nutritional models hinder improvements in feed efficiency for sustainable beef production. This review examines the progression from traditional data collection methods to modern genetic and nutritional approaches that enhance feed efficiency. We first discuss the technological advancements that allow precise measurement of individual feed intake and efficiency, providing valuable insights for research and industry. The role of genomic selection in identifying and breeding feed-efficient animals is then explored, emphasizing the benefits of combining data from multiple populations to enhance genomic prediction accuracy. Additionally, the paper highlights the importance of nutritional models that could be used synergistically with genomic selection. Together, these tools allow for optimized feed management in diverse production systems. Combining these approaches also provides a roadmap for reducing input costs and promoting a more sustainable beef industry. [ABSTRACT FROM AUTHOR]

Simple Summary: As in the rest of Europe, the Belgian boar population has been tending to increase even with the African Swine Fever prevalence, posing new challenges in their management by hunting. Indeed, wild boars have been massively culled by hunters and other nature managers, but some stakeholders argued that they are sensitive animals and that their welfare matters. In this article, we explore the management of wild boar hunting through a qualitative sociological survey focusing on damage to the welfare of wild animals and ways of limiting it. By identifying, based on the knowledge of hunting stakeholders, a multitude of issues and avenues for action to limit the harm to the welfare of wild boars, this paper highlights the relevance of an interdisciplinary and transdisciplinary approach to the welfare issues of wild animals. This study advocates including wild animals—wild boars, in this case—as sentient beings whose welfare must be considered and debated in discussions on global health. As in the rest of Europe, the Belgian boar population has been tending to increase, posing new challenges to management by hunting. Moreover, in autumn 2018, the African Swine Fever (ASF) virus penetrated Belgian territory. In response to this so-called overpopulation and to this health crisis, wild boars were massively culled by hunters and other nature managers. The wild boar was then debated by some stakeholders as a sensitive animal and that its welfare mattered even if it must still be killed. In this article, we explore the management of wild boar through a qualitative sociological survey focusing on damage to the welfare of wild animals and ways of limiting it. This survey is part of an innovative process launched by the Walloon Region and the Walloon Council for Animal Welfare (CWBEA). It was conducted in order to qualitatively describe current hunting practices and their impact on animal welfare; it then integrated knowledge from the human sciences into the CWBEA's work; and, finally, those from the hunting world were invited to debate with the usual animal welfare advocates and managers. By identifying, based on the knowledge of hunting stakeholders and wildlife specialists, a multitude of issues and avenues for action to limit the harm to the welfare of wild boars, this paper highlights the relevance of an interdisciplinary and transdisciplinary approach to the welfare issues of wild animals. It supports the hypothesis that animal welfare advocates can work toward establishing new norms in human/nonhuman relationships in collaboration with hunters but also to include wild animals—wild boars in this case—as sentient beings whose welfare must be considered and debated in discussions on global health. [ABSTRACT FROM AUTHOR]

Simple Summary: Accurate identification of individual Amur tigers is vital for their conservation, as it helps us understand their population and distribution. Existing identification methods often fall short in accuracy, and our study focuses on creating a more accurate method for identifying individual Amur tigers using advanced deep learning techniques. We improved an existing neural network model called InceptionResNetV2 by adding features like dropout layers and dual-attention mechanisms to better capture the unique stripe patterns of each tiger and reduce errors during training. We tested our model on a large dataset of tiger images and found it to be highly effective, achieving an average recognition accuracy of over 95% for different body parts, with left stripes reaching the highest 99.37%. This method significantly outperforms previous models and provides a reliable tool for wildlife researchers and conservationists to monitor and protect Amur tigers. By improving the ability to track individual tigers, our research offers practical benefits for preserving this endangered species and enhancing wildlife management practices globally. Accurate and intelligent identification of rare and endangered individuals of flagship wildlife species, such as Amur tiger (Panthera tigris altaica), is crucial for understanding population structure and distribution, thereby facilitating targeted conservation measures. However, many mathematical modeling methods, including deep learning models, often yield unsatisfactory results. This paper proposes an individual recognition method for Amur tigers based on an improved InceptionResNetV2 model. Initially, the YOLOv5 model is employed to automatically detect and segment facial, left stripe, and right stripe areas from images of 107 individual Amur tigers, achieving a high average classification accuracy of 97.3%. By introducing a dropout layer and a dual-attention mechanism, we enhance the InceptionResNetV2 model to better capture the stripe features of individual tigers at various granularities and reduce overfitting during training. Experimental results demonstrate that our model outperforms other classic models, offering optimal recognition accuracy and ideal loss changes. The average recognition accuracy for different body part features is 95.36%, with left stripes achieving a peak accuracy of 99.37%. These results highlight the model's excellent recognition capabilities. Our research provides a valuable and practical approach to the individual identification of rare and endangered animals, offering significant potential for improving conservation efforts. [ABSTRACT FROM AUTHOR]

Simple Summary: Laparoscopy is a minimally invasive surgical technique used to explore and treat conditions within the abdomen. In equids, this procedure is generally performed standing, with animals sedated and restrained in stocks. Laparoscopic ovariectomy in equids has gained popularity as it avoids the risk of general anaesthesia, greatly improves visualisation and manipulation of the ovary and its blood supply, reduces the recovery period, and provides a superior aesthetic result. Indications for ovariectomy include neutering, fertility problems, ovarian tumours, hematomas or cysts, disorders of sexual development, and the elimination of undesirable behaviour related to oestrus. During the procedure, pain management is achieved with a combination of systemic or loco-regional anaesthesia. The objective of this systematic review was to evaluate studies in the equine veterinary literature, published between 2003 and 2023, in which loco-regional anaesthesia was used during standing laparoscopic ovariectomy in equids to assess the different drugs, techniques, and outcomes. Laparoscopic ovariectomy is generally performed with equids in the standing position, with the animals heavily sedated and restrained in stocks. This procedure may be quite painful, and it is essential first to manage intraoperative pain to complete the surgery, respecting the animal's welfare and, at the same time, ensuring the safety of the operators. Laparoscopy requires multiple small incisions to introduce the instruments, with one to two incisions enlarged sufficiently to remove the ovary. The surgical procedure must be associated with effective pain control, usually obtained with loco-regional anaesthesia, mesovarian injection, mesovaric or ovarian topical anaesthesia, and epidural anaesthesia. This systematic review aims to discuss articles published from 2003 to 2023 on treating loco-regional anaesthesia in standing laparoscopic ovariectomy in association with an evaluation of pain. The literature review was undertaken according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines on three databases (NCBI-PubMed, Web of Science, and SciVerse Scopus). Despite the collected papers numbering 36, we identified only five eligible papers, demonstrating that few studies are performed in order to evaluate the quality of analgesia with loco-regional anaesthesia in standing laparoscopic ovariectomy in equids. The authors of this systematic review agree that the association of injectable and epidural anaesthesia is the best solution to manage intraoperative pain in standing laparoscopic ovariectomy in equids. [ABSTRACT FROM AUTHOR]

This document is a postscript to a collection of papers that explore the effects of human actions on the welfare of invertebrates in various contexts. The papers discuss topics such as breeding facilities, research laboratories, and the wild. The document emphasizes the importance of considering the welfare of invertebrates, both in captivity and in the wild, and highlights the need for more research and information on this topic. It also addresses the ethical considerations and attitudes towards invertebrates, as well as the impact of human activities on wild invertebrates. The document concludes by emphasizing the significant influence humans have on the planet and the need to prioritize the welfare of invertebrates. [Extracted from the article]

Simple Summary: Today's zoos and aquariums claim to be shifting from being entertainment facilities to centers for the conservation of biodiversity. To make this shift, scientific research, including understanding the biology of endangered species and improving the environment of captive animals, is essential. Several studies have examined the trends in the number of research papers published by zoos and aquariums globally and indicated that this number has increased over the past few decades. In this study, we examined the trends in the number of papers published by Japanese zoos and aquariums over the past 62 years to determine whether research activities are also developing in Japan. We found that the number of research papers has significantly increased and that the research fields have diversified since around 1990. However, we also found some problems: about a quarter of the institutions have published no papers, research targets in zoos were biased toward captive mammals and that aquariums conducted little research on animal welfare. Addressing these issues, we would argue, will help Japanese zoos and aquariums to make further progress. With the shift in their social roles, modern zoos and aquariums are required to develop scientific research. Although zoos and aquariums worldwide have reported an increase in the number of papers they publish and the diversification of their fields in recent decades, the specific circumstances in Japan are slightly unclear. We listed peer-reviewed papers authored by Japanese zoos and aquariums using search engines and quantitatively evaluated the changes in the number of papers published over 62 years. Our results showed that papers published in Japan have increased remarkably since the 1990s, and research fields have diversified as in the rest of the world. In particular, joint research with research institutes has seen an upward trend, and the instances of English-language papers have increased. Meanwhile, the content of the research was biased. In zoos, research on animal welfare has been increasing, but the focus was heavily biased toward captive mammals. Aquariums contributed to the understanding of local ecosystems through the fundamental study of wildlife, but there were fewer papers on improving husbandry. Our results indicated that while research by Japanese zoos and aquariums is developing, research on welfare, conservation, and education regarding native endangered species must still be improved. [ABSTRACT FROM AUTHOR]

Simple Summary: Visitors in zoos have a variable impact on animals; principally categorised as positive, negative or neutral. This paper quantifies the impact of visitors in non-primate species, based on 105 papers found in the literature. In total, there were 252 non-primate species studied. The number of papers published has increased since 2012, with a range of animal groups now assessed (including avian, reptilian, amphibian, fish, mammalian and invertebrates). Amphibians responded negatively to visitors more frequently than would be expected, birds responded neutrally more frequently than would be expected and fish responded neutrally and 'unknown' more frequently than would be expected. A number of animal-based metrics have been used to assess the impacts of visitors on animals, with measures used varying across taxa. It is recommended that moving forwards researchers incorporate a suite of measures, particularly those which are meaningful in terms of being representative of individual animal experiences and animal welfare, collected in a manner which should capture those metrics accurately. Visitors are a prominent feature in the lives of zoo animals, and their presence can cause a range of impacts on zoo animals (typically classed as positive, negative or neutral impacts), commonly referred to as the 'visitor effect'. This paper quantitatively collates the literature on the visitor effect in non-primate species, investigates the types of measures used to assess impacts of visitors on animals and considers whether impacts vary across non-primate species in zoos. In total, there were 105 papers which had investigated the impact of zoo visitors on 252 non-primate species/species groups. There has been a steady increase in visitor effect research in zoos since 2012 and this body of work incorporates species from avian (28% study species), reptilian (9%), amphibian (2%), fish (4%) and invertebrate taxa (1%). However, there is still a bias towards mammalian species (56%). The response to visitors varied across taxa. Amphibians responded negatively to visitors more frequently than would be expected by chance (p < 0.05), birds responded neutrally more frequently than would be expected by chance (p < 0.05) and fish responded neutrally and 'unknown' more frequently than would be expected by chance (p < 0.05). This review highlighted a number of animal-based metrics which have been used to assess the impacts of visitors on animals, with measures used varying across taxa. Moving forwards, it is recommended that moving forwards researchers incorporate a suite of measures, incorporating those which are meaningful in terms of being representative of individual animal experiences and animal welfare, collected in a manner which should capture those metrics accurately. [ABSTRACT FROM AUTHOR]

Simple Summary: On 14 January 2016, Daniel Brighton killed one of two dogs that had strayed onto his property, where they had attacked Alice, a camel owned by a Petting Zoo business operated by Brighton. In late August 2017, the Royal Society for the Prevention of Cruelty to Animals, New South Wales (RSPCA) became aware of the allegations, commenced an investigation, and thereafter preferred charges in the NSW Local Court at Campbelltown. As was his right, Brighton pleaded not guilty to two charges, brought pursuant to s 530 of the Crimes Act 1900 (NSW), which alleged that he committed acts of serious animal cruelty. The charges particularised offences that, whilst intending to inflict severe pain on the dog, Brighton firstly seriously injured the dog by stabbing him with a pitchfork several times and secondly, that he subsequently killed the dog by hanging him from a tree and striking him with a mallet. Brighton maintained his not guilty position until 13 December 2023, when during the hearing of the second of two Supreme Court Appeals, before Hamill J, he withdrew that appeal (against the conviction), as well as a concurrent appeal in the District Court (also against conviction and sentence). The Hamill J decision was published on 12 January 2024, two days shy of the eighth anniversary of the attack on Alice and the dog's slow and painful death. Animal law has the potential to initiate improvements for animal wellbeing. However, this largely depends on how effectively the law bridges the legal chasm between animal welfare and animal suffering, a chasm the authors refer to as the welfare gap. When the law does not adequately address this gap, where regulation subordinates animal interests to human interests, it results in weak animal protection that does little more than regulate to a standard that avoids a life not worth living. The authors analyse a series of cases involving the RSPCA and Brighton, in which Brighton was charged with serious animal cruelty pursuant to s 530 of the Crimes Act 1900 (NSW). He stabbed a dog with a pitchfork; after failing to kill the dog, he suspended it from a tree branch by a leash attached to its collar and struck the dog several times on the head with a mallet, finally killing him. Brighton was found guilty in the NSW Local Court and appealed to the NSW Supreme Court, where Rothman J allowed the appeal, holding that Brighton had exterminated a pest animal. This led to protracted litigation, including to the NSW Court of Appeal, a second hearing in the Local Court and a further appeal to the Supreme Court. In August 2020, Sophie Riley published a case note and commentary on the litigation up to the Rothman J appeal. This paper evaluates the litigation that followed, identifying how regulatory failures have entrenched the welfare gap. Regulatory failures include inadequate and aged legislative protections for a confined subset of animals. In NSW, animal sentience is not enshrined in legislation; the law limits the types of animals protected by anti-cruelty law; fundamental statutory language remains undefined, for example terms such as "pest animal" and "exterminate"; and challenges abound for adducing sufficient evidence to prove subjective criminal intent. These deficiencies pose significant challenges for practitioners and judicial officers, particularly when complex statutory interpretation is required in the busy and fast-paced summary jurisdiction. This paper concludes that legislators should consider modernising the law, removing ambiguity, and settling minimum standards for a good life for animals, taking into account the welfare aspects described in Mellor's Five Domains model. [ABSTRACT FROM AUTHOR]

The document discusses the impact of antimicrobial usage on animal health, emphasizing the emergence of drug-resistant bacteria due to excessive and uncontrolled use of antibiotics. It highlights the importance of antimicrobial stewardship and monitoring systems to address antimicrobial resistance, with a focus on the interconnectedness of human, animal, and environmental health. The document includes research papers on monitoring antimicrobial usage, antibiotic stewardship interventions, and detecting antimicrobial resistance in bacteria from various sources, providing insights into strategies to mitigate antimicrobial use and resistance in animals. The Special Issue aims to encourage the development of new antibiotic alternatives and enhance antimicrobial stewardship practices globally. [Extracted from the article]

Simple Summary: Lactococcus petauri has been described for the first time in 2017 in a sugar glider. A few years later, it has emerged as important pathogen for fish and more recently it has been reported also in terrestrial mammals and humans. A lot of these cases were misdiagnosed as Lactococcus garvieae infection due to the limitation of the available common standard diagnostic techniques used which were unable to discriminate between these two species and relatively high similarity of the two pathogens at genetic and phenotypic levels. Till today, there are only two reported cases of lactococcosis in dogs with no previous cases were reported from Italy. This report is the first description of L. petauri infection in two domestics dog cases from Italy. The results of the current report provide an update on the host tropism, and additional knowledge on virulence factors and antimicrobial resistance of L. petauri. Lactococcus garvieae has been considered for a long time the only causal agent of lactococcosis. In recent years, different papers reported the involvement of other two bacterial species: Lactococcus petauri and Lactococcus formosensis. A different host tropism has been described for these species where L. garvieae and L. petauri are predominant species in fish and humans' infections, while L. formosensis in bovine. L. garvieae has been reported as rare infectious agent in dog. This paper represents the first isolation of L. petauri in two domesticated dog cases from urine and skin samples, respectively. The recovered L. petauri has been identified using PCR and sequencing based on Internal Transcribe Spacer (ITS) and phylogenetic analysis showed that it belongs to the L. petauri cluster with a 100% of identity with sequences previously reported from fish isolates while there were differences with L. petauri isolated from urinary tract infection from humans. L. petauri in human infection has been considered not necessarily deriving from the ingestion of contaminated food but rather as an opportunistic pathogen colonization intestinal tract. Differences among virulotypes have been reported for humans and dogs, and a comparison was also made between the virulotyping of L. petauri and L. garvieae in dogs. The antimicrobial pattern showed susceptibility for the election treatment molecules. These data contribute to our understanding of the host trophism of this species which was misclassified for long time and provide new data on its virulence factors and antimicrobial resistance. [ABSTRACT FROM AUTHOR]

Simple Summary: In the mammalian immune system, αβ T lymphocytes are a fundamental set of T cells capable of recognizing an extraordinary range of antigens, presented in the context of the major histocompatibility complex molecules. The function of αβ T cells is linked to the T-cell receptor, a heterodimeric cell surface protein composed of an α and β chain uniquely expressed on T cells. Unlike conventional proteins encoded by a single gene, α and β chains are encoded by a large set of noncontiguous TRA and TRB genes that are randomly assembled during a stochastic process known as V(D)J recombination, generating a large and diverse repertoire. The extent of diversification depends on the number of genes and their genomic organization at the TRA and TRB loci, which varies from species to species. In this paper, we describe the genomic organization and gene content of the horse TRB locus, based on the recently released horse genome. Compared to other mammalian species, the horse TRB locus was found to be the largest locus studied to date. A clonotype analysis of a transcriptomic dataset was also performed to assess the characteristics of the V(D)J somatic rearrangement. Overall, our data provide useful information for further functional studies in healthy and sick horses of different breeds. In this paper, we report a comprehensive and consistent annotation of the locus encoding the β-chain of the equine T-cell receptor (TRB), as inferred from recent genome assembly using bioinformatics tools. The horse TRB locus spans approximately 1 Mb, making it the largest locus among the mammalian species studied to date, with a significantly higher number of genes related to extensive duplicative events. In the region, 136 TRBV (belonging to 29 subgroups), 2 TRBD, 13 TRBJ, and 2 TRBC genes, were identified. The general genomic organization resembles that of other mammals, with a V cluster of 135 TRBV genes located upstream of two in-tandem aligned TRBD-J-C clusters and an inverted TRBV gene at the 3′ end of the last TRBC gene. However, the horse b-chain repertoire would be affected by a high number of non-functional TRBV genes. Thus, we queried a transcriptomic dataset derived from splenic tissue of a healthy adult horse, using each TRBJ gene as a probe to analyze clonotypes encompassing the V(D)J junction. This analysis provided insights into the usage of the TRBV, TRBD, and TRBJ genes and the variability of the non-germline-encoded CDR3. Our results clearly demonstrated that the horse β-chain constitutes a complex level of variability, broadly like that described in other mammalian species. [ABSTRACT FROM AUTHOR]

Simple Summary: Age at puberty is a main factor affecting reproductive efficiency and, thus, livestock production. Besides species and breed, nutrition during growth plays a crucial role in the attainment of puberty. Controversial results are reported about the critical period during which heifers are more sensitive to the effects of nutrition on pubertal acceleration. Numerous papers are available about such topics, but they are characterized by very different experimental conditions (such as genetics, feeds, planes of nutrition, season and pasture quality, time windows, and parameters considered), making difficult the comparison among inconstant results by various trials. Several indicators during growth have been proposed as predictors of age at puberty, such as body weight gains, body fat reserves, and linear body measurements. Blood concentrations of hormones strictly linked to growth and reproduction have also been considered: higher Insulin-like Growth Factor-1 levels are reported to be related to earlier puberty, while leptin is not. More uniform experimental conditions should be encouraged for future research. Puberty onset and age at first calving have a critical impact on livestock production for good reproductive efficiency of the herd and to reduce the duration of the non-productive stage of the growing heifer. Besides genetic factors, sexual maturation is also affected by environmental factors, such as nutrition, which can account for up to 20% of the observed variability. The rate of body weight gain during growth is considered the main variable influencing the age at puberty, dependent on planes of nutrition in growing animals during the prepubertal-to-pregnancy stage. This paper reviews current knowledge concerning nutrition management and attainment of puberty in heifers, considering the relevance of some indicators such as body measurements and hormones strictly linked to the growth and puberty process. Puberty onset is dependent on the acquisition of adequate subcutaneous adipose tissue mass, as it is the main source of the hormone leptin. Until a certain level, body condition score and age at puberty are negatively correlated, but beyond that, for fatter animals, such correlation is gradually lost. Age at puberty in heifers was reported to be negatively related to IGF-1. Future research should be planned considering the need to standardize the experimental animals and conditions. [ABSTRACT FROM AUTHOR]

Simple Summary: Cattle behavior recognition is an important field in animal husbandry. It can be used to understand the health status, emotions and needs of cattle. In this paper, an accurate and lightweight behavioral multi-detection model is proposed, which is adapted to real weather conditions. An innovation in the head, neck, detection head and loss function of the model is proposed, which improves the accuracy of behavior detection in cattle, and greatly reduces the number of parameters and calculations. It not only has high accuracy in recognition tasks, but is also very friendly to edge devices. This gives breeders insight into cattle behavior, helping them to better manage their herds, improve breeding efficiency and ensure the health and welfare of their cattle. In modern animal husbandry, intelligent digital farming has become the key to improve production efficiency. This paper introduces a model based on improved YOLOv8, Cattle Behavior Recognition-YOLO (CBR-YOLO), which aims to accurately identify the behavior of cattle. We not only generate a variety of weather conditions, but also introduce multi-target detection technology to achieve comprehensive monitoring of cattle and their status. We introduce Inner-MPDIoU Loss and we have innovatively designed the Multi-Convolutional Focused Pyramid module to explore and learn in depth the detailed features of cattle in different states. Meanwhile, the Lightweight Multi-Scale Feature Fusion Detection Head module is proposed to take advantage of deep convolution, achieving a lightweight network architecture and effectively reducing redundant information. Experimental results prove that our method achieves an average accuracy of 90.2% with a reduction of 3.9 G floating-point numbers, an increase of 7.4%, significantly better than 12 kinds of SOTA object detection models. By deploying our approach on monitoring computers on farms, we expect to advance the development of automated cattle monitoring systems to improve animal welfare and farm management. [ABSTRACT FROM AUTHOR]

Simple Summary: The Syringophilidae family is one of the most diverse groups of prostigmatic mites (Acari: Prostigmata) parasitising birds. In this paper, we describe a new genus and species, Corvisyringophilus krummi, which parasitises the Common Raven (Corvus corax) in Iceland. This new genus belongs to a group of primitive genera that retain a complete set of idiosomal and leg setae. Our phylogenetic analysis suggests that Corvisyringophilus is morphologically closely related to the genera Blaszakia Skoracki & Sikora, 2008 and Charadriphilus Bochkov & Mironov, 1998, which are associated with birds of different and phylogenetically unrelated orders: Musophagiformes and Charadriiformes, respectively. This research highlights the importance of examining the genus Corvus in order to better understand the specialised host–parasite relationships. Furthermore, the study emphasises the significance of using museum collections to study parasitic interactions. Syringophilidae is one of the most species-rich families in the superfamily Cheyletoidea, comprising approximately 420 species across 62 genera and two subfamilies. In this paper, we propose a new genus, Corvisyringophilus, and a new species, C. krummi gen. n. et sp. n., found in the wing covert quills of the Common Raven, Corvus corax Linnaeus, in Iceland. Corvisyringophilus is placed among the primitive genera of syringophilid mites, which possess the full complement of idiosomal and leg setae. Phylogenetic analysis based on morphological characters suggests that this genus forms a sister clade to Blaszakia Skoracki & Sikora, 2008, and Charadriphilus Bochkov & Mironov, 1998, which inhabit birds of the orders Musophagiformes and Charadriiformes, respectively. The study proposes that the current distribution patterns of quill mites, based on their morphological characteristics, may result from multiple host switching followed by co-speciation events, highlighting the complex evolutionary dynamics within this family. [ABSTRACT FROM AUTHOR]

Simple Summary: One may think that the number of pigs that can fit on a truck is a simple scientific question. It is not. It is vitally important that scientists can describe their work simply and concisely to the public, especially when that work is providing the basis for regulation in the public interest. Successful regulation of human behavior requires the regulated to agree with the rule. Commercial livestock transporters have an interest in loading a livestock compartment to the maximum to minimize costs. Livestock production academics, veterinarians, and animal welfare activists have been working for decades to determine the level of livestock crowding in transport containers that would be an appropriate threshold for regulatory enforcement. To date, there is no real consensus on this issue across species or animal size within a species. The EU countries agreed to a maximum floor pressure for market pigs of around 110 kg body weight to be 235 kgm−2 in 2005, while North American regulators have no legal standard. Current practice in North America allows for significant crowding of pigs in excess of the EU standard. Using the pig as an example, this paper examines the practical barriers that have for decades prevented emergence of a consensus on the "Is this truck full?" question. This paper focuses on the problem of numeracy when writing regulations, specifically how to describe a threshold for crowding of pigs during transport, considering transported pigs range in body mass from 5 to 500 kg. When scientific findings provide the basis for regulation in the public interest, those findings must be communicated in a consistent way to regulators and policymaking bodies. Numeracy is the ability to understand, reason with, and apply appropriate numerical concepts to real-world questions. Scientific understanding is almost always based on rational understanding of numerical information, numeracy. The threshold of administrative offenses is often a numerical description. Commercial livestock transporters have an interest in loading livestock compartments to the maximum to achieve the largest payload allowed by axle weight laws, as is the case in all bulk commodity transport. Maximizing payload minimizes costs and environmental hazards of fuel exhaust and can benefit the public with lower pork prices, but has a serious animal welfare risk. Livestock production academics, veterinarians, and animal welfare activists have been working for decades to determine the level of livestock crowding in transport containers that would be appropriate for regulatory enforcement. The scientific discourse has been plagued by a lack of numerical standardization when describing results of trials and forming recommendations. Exceeding specific numerical thresholds is the core to implementing enforcement actions. This paper examines the communication and other barriers that have prevented emergence of a consensus on this question and provides a direction toward resolution. Further confirmation of effects of crowding livestock in transit is needed. This paper suggests that articulating an enforceable standard in pig transport is possible. In inspection for compliance, discovering the LP50 (lethal pressure—50) for slaughter-weight pigs is an initial global benchmark goal. The LP50 is the loading floor pressure in a commercial transport compartment, under field conditions, that would result in the death of at least one pig in the group 50% of the time. [ABSTRACT FROM AUTHOR]

Simple Summary: Infrared thermography is a rapid, contactless technology that can provide real-time temperature measurements, with various applications in livestock science within the framework of precision livestock farming. The aim of the current review paper is to present the updated state of knowledge regarding the detection of mastitis in dairy ruminants using infrared thermography. Therefore, it summarizes the technological advancements, the diagnostic capacity, and the operational and analytical challenges of the method when applied under real-world conditions as a diagnostic tool for mastitis, while factors associated with the performance of the method are critically described and discussed to justify recognized strengths, weaknesses, opportunities, and challenges of the method. Among the health issues of major concern in dairy ruminants, mastitis stands out as being associated with considerable losses in productivity and compromised animal health and welfare. Currently, the available methods for the early detection of mastitis are either inaccurate, requiring further validation, or expensive and labor intensive. Moreover, most of them cannot be applied at the point of care. Infrared thermography (IRT) is a rapid, non-invasive technology that can be used in situ to measure udder temperature and identify variations and inconsistencies thereof, serving as a benchmarking tool for the assessment of udders' physiological and/or health status. Despite the numerous applications in livestock farming, IRT is still underexploited due to the lack of standardized operation procedures and significant gaps regarding the optimum settings of the thermal cameras, which are currently exploited on a case-specific basis. Therefore, the objective of this review paper was twofold: first, to provide the state of knowledge on the applications of IRT for the assessment of udder health status in dairy ruminants, and second, to summarize and discuss the major strengths and weaknesses of IRT application at the point of care, as well as future challenges and opportunities of its extensive adoption for the diagnosis of udder health status and control of mastitis at the animal and herd levels. [ABSTRACT FROM AUTHOR]

Simple Summary: This paper aims to review the development of the cow–calf bonding process in water buffalo (Bubalus bubalis) via olfactory, tactile, auditory, and visual stimuli. It will also discuss the neuroendocrine factors motivating buffalo cows to care for the calf. To develop cow–calf bonding, several olfactory, tactile, auditory, and visual cues need to be interchanged and processed by both the mother and the newborn. Sniffing, licking, grooming, and listening to the calf's vocalizations are the first inputs during calving. Oxytocin is the main hormone that mediates selective maternal behavior. Understanding the importance of the sensitive period and the endocrine changes required to elicit maternal behavior could help provide the appropriate stimulus to both the buffalo cow and the calf during calving. The cow–calf bonding is a process that must be developed within the first six hours after calving. Both the buffalo dam and the newborn calf receive a series of sensory cues during calving, including olfactory, tactile, auditory, and visual stimuli. These inputs are processed in the brain to develop an exclusive bond where the dam provides selective care to the filial newborn. The limbic system, sensory cortices, and maternal-related hormones such as oxytocin mediate this process. Due to the complex integration of the maternal response towards the newborn, this paper aims to review the development of the cow–calf bonding process in water buffalo (Bubalus bubalis) via the olfactory, tactile, auditory, and visual stimuli. It will also discuss the neuroendocrine factors motivating buffalo cows to care for the calf using examples in other ruminant species where dam–newborn bonding has been extensively studied. [ABSTRACT FROM AUTHOR]

Simple Summary: Genomic selection (GS) is defined as a marker-assisted selection method that uses high-density molecular markers covering the entire genome combined with phenotypic data to estimate genomic estimated breeding values (GEBV). This paper reviews the principles, methods, and influencing factors of GS for breeding by using phenotypic information and genetic relationships simultaneously. It also covers the research progress of GS in meat, fiber, dairy, and reproductive traits in sheep and goats. Compared to other livestock, especially cattle, sheep and goat breeding is relatively small-scale and has lagged behind. GS combined with genomic information can help promote the breeding process and development in commercial farming of sheep and goats. We highlight the potential for phenotypic information and genetic relationships to work together to drive the breeding process, providing valuable insights for implementing GS in sheep and goat breeding. The advancement of sequencing technology and molecular breeding methods has provided technical support and assurance for accurate breeding. Genomic Selection (GS) utilizes genomic information to improve livestock breeding, and it is more accurate and more efficient than traditional selection methods. GS has been widely applied in domestic animal breeding, especially in cattle. However, there are still limited studies on the application and research of GS in sheep and goats. This paper outlines the principles, analysis methods, and influential factors of GS and elaborates on the research progress, challenges, and prospects of applying GS in sheep and goat breeding. Through the review of these aspects, this paper is expected to provide valuable references for the implementation of GS in the field of sheep and goat breeding. [ABSTRACT FROM AUTHOR]

Simple Summary: Porcine reproductive and respiratory syndrome virus (PRRSV) infection in sows results in reduced animal welfare, poor breeding herd performance, and economic loss to farmers. The best way to reduce the effects of PRRSV is to prevent its entry into swine breeding herds through improvements in farm biosecurity. To accomplish this goal, the concept of Next Generation Biosecurity (NGB) was developed. A recent publication demonstrated that the practice of a comprehensive, science-based program of biosecurity (NGB COMPLETE) which incorporated science-based biosecurity protocols targeting direct and indirect routes of PRRSV transmission, significantly reduced PRRSV incidence risk in breeding herds compared to a partial program (NGB INCOMPLETE). This new communication follows up on this earlier paper and brings new information by reporting significant differences in key performance indicators between NGB COMPLETE breeding herds versus NGB INCOMPLETE herds over that original 2-year period across the same swine production system. It also supplements the earlier paper with data from a third consecutive year of reduced PRRSV incidence risk, resulting in a PRRSV incidence risk of 8.0% across all breeding herds for the entire three years. In closing, this is further evidence demonstrating that NGB, while not perfect, brings value to farmers through sustainable prevention of PRRS and improved productivity. Porcine reproductive and respiratory syndrome virus (PRRSV) is a globally significant pathogen of pigs. Preventing the entry of PRRSV into swine breeding herds enhances animal health and welfare. A recently published retrospective cohort study reported significant differences in PRRSV incidence risk between breeding herds that practiced Next Generation Biosecurity (NGB) COMPLETE, versus herds that practiced a partial approach (NGB INCOMPLETE) over a 2-year period. This follow-up communication builds on this previous publication and brings new information regarding statistical differences in key performance indicators (KPIs) from 43 NGB COMPLETE herds and 19 NGB INCOMPLETE herds during disease years 1 and 2. Statistically significant differences included higher total born/farrow and pigs weaned/female along with a reduced pre-weaning mortality and wean to 1st service interval, as well as a 0.91 increase in the number of pigs weaned/mated female/year. In addition, this communication reports that PRRSV incidence risk throughout disease years 1–3 was 8.0%, and that the association of NGB status (COMPLETE vs. INCOMPLETE) and disease burden for the cumulative 3-year period was statistically significant (p < 0.0001). These findings support previously published data that NGB, while not perfect, provides sustainable prevention of PRRSV, and may help improve herd productivity. [ABSTRACT FROM AUTHOR]

Simple Summary: In the process of large-scale cage chicken breeding, using inspection robots instead of manual detection can solve the problems of large workload and low detection efficiency. However, poor driving stability of an inspection robot will lead to low inspection accuracy. In order to ensure high efficiency, accuracy, and stability of detection, this study takes the stability of visual navigation for inspection robots as the optimization goal, and designs an efficient and accurate road extraction algorithm and a navigation line fitting algorithm with better robustness. The experimental results show that the algorithm proposed in this study can improve the stability of detection, achieve a faster running speed, and achieve a better detection effect and a higher detection efficiency while keeping the detection effect unchanged. The navigation algorithm can accelerate the automation process of large-scale cage chicken breeding and promote the realization of fast and accurate monitoring. The speed and accuracy of navigation road extraction and driving stability affect the inspection accuracy of cage chicken coop inspection robots. In this paper, a new grayscale factor (4B-3R-2G) was proposed to achieve fast and accurate road extraction, and a navigation line fitting algorithm based on the road boundary features was proposed to improve the stability of the algorithm. The proposed grayscale factor achieved 92.918% segmentation accuracy, and the speed was six times faster than the deep learning model. The experimental results showed that at the speed of 0.348 m/s, the maximum deviation of the visual navigation was 4 cm, the average deviation was 1.561 cm, the maximum acceleration was 1.122 m/s2, and the average acceleration was 0.292 m/s2, with the detection number and accuracy increased by 21.125% and 1.228%, respectively. Compared with inertial navigation, visual navigation can significantly improve the navigation accuracy and stability of the inspection robot and lead to better inspection effects. The visual navigation system proposed in this paper has better driving stability, higher inspection efficiency, better inspection effect, and lower operating costs, which is of great significance to promote the automation process of large-scale cage chicken breeding and realize rapid and accurate monitoring. [ABSTRACT FROM AUTHOR]

Simple Summary: Historically, one of the strategies the Myanmar Government employed to resolve human–elephant conflict was the capture of whole herds of elephants using the Kheddah system. This involved trapping the herd in a stockade, immediately followed by on-site post-capture taming. After taming, the captured elephants were trained to be utilized as logging elephants. The capture of wild elephants was formally banned in Myanmar in 1985, but occasional, small-scale, captures by the Kheddah system were allowed until 2013. These captures were under the strict control of the Myanmar Government and focused primarily on elephants involved in human–elephant conflict, rather than capturing to supplement the working elephant population. The method itself has largely fallen out of use because capturing an ecologically important, iconic animal is unacceptable and against current welfare requirements. This paper attempts to record the Kheddah system of elephant capture that was employed when the individually selected capture method was impractical for the emergency removal of a herd of marauding elephants. The author presents a description and assessment of the Kheddah method not to endorse it, but to document and discuss a method that was part of Myanmar's elephant-keeping history before it is forgotten. When standard methods of human–elephant conflict mitigation are not successful, free-ranging wild elephants may continue to come into close contact with people. This results in more frequent and severe conflict, with consequences ranging from crop raiding to loss of human and elephant lives. Understandably, in such situations, local communities may want to be rid of entire herds of elephants. Historically, one of the strategies the Myanmar Government practiced to resolve human–elephant conflict was the capture of whole herds using the Kheddah system. This involved trapping the herd in a stockade, immediately followed by on-site post-capture taming. After taming, the captured elephants were utilized as logging elephants. Elephants worked in timber extraction, retired at age 55 years and were then cared for by the Government until they died. The capture of wild elephants by the Kheddah system was formally banned in Myanmar in 1985 but occasional, small-scale, captures were allowed until 2013 under the strict control of the Myanmar Government. These operations were aimed primarily at capturing elephants involved in human–elephant conflict, rather than to supplement the working elephant population. One of the last Kheddah operations, organized and managed by the author, was conducted in 1996 at the Taikkyi township of the Yangon Region, as a last resort to end human–elephant conflict in an emergency. While chemical immobilization was being used at this time, it was not logistically possible with the high numbers of elephants engaged in the conflict. This review aims to record the history of an activity that was an important element of Myanmar's timber industry more than three decades ago. In this paper, the author presents a description of Kheddah not to endorse it, but to document (1) the Myanmar elephant population management strategy in the past, before it is forgotten, and (2) the practicality of the Kheddha operation when the singly selected commonly- used immobilization or noosing method of elephant capture is unfeasible. The author attempts to shed light on the modern veterinary procedures that may significantly reduce the notorious historical outcome of Kheddha, especially the resulting mortality of captured elephants, should the Kheddha system of capture ever be used as an emergency solution for ongoing problems of human–elephant conflict in the range states of Asia. [ABSTRACT FROM AUTHOR]