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1. Using RNA-Seq to Identify Reference Genes of the Transition from Brown to White Adipose Tissue in Goats

Author

Linjie Wang, Xingyue Chen, Tianzeng Song, Xujia Zhang, Siyuan Zhan, Jiaxue Cao, Tao Zhong, Jiazhong Guo, Li Li, Hongping Zhang, and Yan Wang

Subjects
goat, brown adipose tissue, reference gene, Normfinder, BestKeeper, geNorm, Veterinary medicine, SF600-1100, Zoology, QL1-991
Abstract

Brown adipose tissues have unique non-shivering thermogenesis functions, can be found in newborn ruminate animals, and then are gradually replaced by white adipose tissues in adulthood. For the purpose of exploring the intrinsic mechanism underlying the conversion process from brown (BAT) to white adipose tissue (WAT), it is necessary to utilize Quantitative PCR (qPCR) to study gene expression profiling. In this study, we identified reference genes that were consistently expressed during the transformation from goat BAT to WAT using RNA-seq data. Then, twelve genes were evaluated as candidate reference genes for qPCR in goat perirenal adipose tissue using three tools (geNorm, Normfinder, and BestKeeper). In addition, the selected reference genes were used to normalize the gene expression of PGC-1α and GPAT4. It was found that traditional reference genes, such as GAPDH, RPLP0, HPRT1, and PPIA were not suitable for target gene normalization. In contrast, CTNNB, PFDN5, and EIF3M, selected from RNA sequencing data, showed the least variation and were recommended as the best reference genes during the transformation from BAT to WAT.

Published
2020
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2. Using RNA-Seq to Identify Reference Genes of the Transition from Brown to White Adipose Tissue in Goats

Author

Tianzeng Song, Siyuan Zhan, Jiazhong Guo, Xujia Zhang, Yan Wang, Jiaxue Cao, Li Li, Xingyue Chen, Tao Zhong, Hongping Zhang, and Linjie Wang

Subjects
Genetics, BestKeeper, lcsh:Veterinary medicine, General Veterinary, goat, reference gene, Adipose tissue, RNA-Seq, brown adipose tissue, White adipose tissue, Biology, geNorm, Article, Gene expression profiling, medicine.anatomical_structure, Reference genes, Normfinder, Gene expression, Brown adipose tissue, lcsh:Zoology, medicine, lcsh:SF600-1100, Animal Science and Zoology, lcsh:QL1-991, Gene
Abstract

Brown adipose tissues have unique non-shivering thermogenesis functions, can be found in newborn ruminate animals, and then are gradually replaced by white adipose tissues in adulthood. For the purpose of exploring the intrinsic mechanism underlying the conversion process from brown (BAT) to white adipose tissue (WAT), it is necessary to utilize Quantitative PCR (qPCR) to study gene expression profiling. In this study, we identified reference genes that were consistently expressed during the transformation from goat BAT to WAT using RNA-seq data. Then, twelve genes were evaluated as candidate reference genes for qPCR in goat perirenal adipose tissue using three tools (geNorm, Normfinder, and BestKeeper). In addition, the selected reference genes were used to normalize the gene expression of PGC-1&alpha, and GPAT4. It was found that traditional reference genes, such as GAPDH, RPLP0, HPRT1, and PPIA were not suitable for target gene normalization. In contrast, CTNNB, PFDN5, and EIF3M, selected from RNA sequencing data, showed the least variation and were recommended as the best reference genes during the transformation from BAT to WAT.

Published
2020

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