1. Pro-inflammatory cytokine and acute phase protein responses to low-dose lipopolysaccharide (LPS) challenge in pigs
- Author
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Laura A. Boyle, Sean Arkins, S. Llamas Moya, and P. B. Lynch
- Subjects
medicine.medical_specialty ,Saliva ,biology ,business.industry ,medicine.medical_treatment ,Haptoglobin ,C-reactive protein ,Acute-phase protein ,Endocrinology ,Cytokine ,Internal medicine ,Blood plasma ,medicine ,biology.protein ,Animal Science and Zoology ,Tumor necrosis factor alpha ,Serum amyloid A ,business - Abstract
The objective of this study was to establish the pro-inflammatory cytokine and acute phase protein responses to low-dose lipopolysaccharide (LPS) challenge in pigs and to determine whether these immune parameters could also be measured in saliva. Possible gender differences in the acute phase reaction were also assessed. At 6 weeks of age, 24 male and 24 female pigs were injected intraperitoneally with a single dose of 0 or 5 μg/kg live weight (LW) of LPS fromEscherichia coli(treatment). Matched saliva and blood samples were taken at 0, 2, 4, 8, 12 or 24 h after treatment administration. Samples were analysed for concentrations of the pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-α) and interleukin-1beta (IL-1β), the acute phase proteins C-reactive protein (CRP), serum amyloid A (SAA), haptoglobin (Hp), and cortisol. Low-dose LPS administration increased plasma levels of TNF-α (PPPP>0·1). Treatment by time interactions showed that plasma levels of TNF-α and CRP in LPS-treated pigs peaked at 2 h (PPP=0·056) and the response to LPS differed between genders (PPPP
- Published
- 2006
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