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Start Over Author "Wu Cai" Journal animal reproduction science
10 results on '"Wu Cai"'

5. GDF-9 and bFGF enhance the effect of FSH on the survival, activation, and growth of cattle primordial follicles

Author

Wu-Cai Yang, Ke-Qiong Tang, Can-Jie Wu, Xiang Li, Liguo Yang, and Lei Sang

Subjects
endocrine system, medicine.medical_specialty, Ovarian Cortex, Cell, Basic fibroblast growth factor, Growth Differentiation Factor 9, Biology, Tissue Culture Techniques, chemistry.chemical_compound, Follicle-stimulating hormone, Endocrinology, Ovarian Follicle, Food Animals, Internal medicine, medicine, Animals, TUNEL assay, General Medicine, medicine.anatomical_structure, chemistry, Apoptosis, Cattle, Female, Fibroblast Growth Factor 2, Animal Science and Zoology, Follicle Stimulating Hormone, hormones, hormone substitutes, and hormone antagonists
Abstract

This study aims to investigate the effects of follicle stimulating hormone (FSH) in combination with growth and differentiation factor-9 (GDF-9) or basic fibroblast growth factor (bFGF) on the activation, survival and growth of cattle primordial follicles. Ovarian tissues were cultured for 3, 7, 14, 22 days in α minimum essential medium (α-MEM) supplemented with FSH, FSH+GDF-9 or FSH+bFGF. Non-cultured and cultured ovarian fragments were processed for histological and TUNEL analysis. Compared to the FSH medium, the results showed FSH+GDF-9 medium increased the percentage of primary follicles in all culture periods and secondary follicles after 14 days of culture (P

Published
2012
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6. Polymorphisms in follicle stimulation hormone receptor, inhibin alpha, inhibin bata A, and prolactin genes, and their association with sperm quality in Chinese Holstein bulls

Author

Ke-Qiong Tang, Qing-Zhi Du, Xiao-Xia Zhang, Liguo Yang, Gua-hua Hua, Wu-Cai Yang, Lei Sang, and Jun-Na Yu

Subjects
Male, endocrine system, medicine.medical_specialty, Genotype, Semen, Single-nucleotide polymorphism, Biology, Polymorphism, Single Nucleotide, Follicle, Endocrinology, Quantitative Trait, Heritable, Food Animals, Gene Frequency, Internal medicine, medicine, Animals, Inhibins, Sperm motility, Inhibin-beta Subunits, Polymorphism, Genetic, urogenital system, INHA, General Medicine, Sperm, Spermatozoa, Prolactin, Hormone receptor, Sperm Motility, Receptors, FSH, Animal Science and Zoology, Cattle
Abstract

Candidate genes follicle stimulation hormone receptor (FSHR), inhibin alpha (INHA), inhibin bata A (INHBA) and prolactin (PRL) were investigated for their association with sperm quality traits of semen volume per ejaculate (VOL), sperm concentration (SCON), motility (MOT), sperm motility in frozen semen (FMOT), acrosome integrity rate (AIR) and abnormal sperm rate (ASR) in a total of 123 normal mature Holstein bulls. Three reported single nucleotide polymorphisms (SNPs) of FSHR A-234500T (rs43676359), IHNA A192G (rs41257116), and IHNBA C7639T (rs43408735), and 3 novel SNPs (G7550A, C7661T, and T8370C) in exons 4 and 5 of bovine PRL gene (NC 007324) were analyzed. Analysis of variance revealed that FSHR A-234500T and INHBA C7639T polymorphisms significantly associated with VOL (P

Published
2010

7. Polymorphisms in the 5' upstream region of the FSH receptor gene, and their association with superovulation traits in Chinese Holstein cows

Author

Li Han, Chunyan Zhang, Guohua Hua, Shu-Jing Li, Jun-Na Yu, Liguo Yang, Ke-Qiong Tang, and Wu-Cai Yang

Subjects
Genetic Markers, medicine.medical_specialty, Genotype, Quantitative Trait Loci, Locus (genetics), Single-nucleotide polymorphism, Superovulation, Biology, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Endocrinology, Food Animals, Gene Frequency, Internal medicine, medicine, Animals, Allele, Gene, Allele frequency, Polymorphism, Single-Stranded Conformational, General Medicine, Sequence Analysis, DNA, Receptors, FSH, Animal Science and Zoology, Cattle, Female, Gene polymorphism, Follicle-stimulating hormone receptor, Polymorphism, Restriction Fragment Length
Abstract

To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, variation in the bovine follicle stimulating hormone receptor (FSHR) gene was investigated using PCR-single-strand conformational (PCR-SSCP) and DNA sequencing. One single nucleotide polymorphism (SNP) of G-278A located in the 5' upstream region of bovine FSHR gene was found in 118 Chinese Holstein cows treated for superovulation. Two SNPs of G-278A (GU253337) and A-320T (rs43676359) were analyzed. In polymorphic locus -278, all cows without superovulation response were mutations with genotypes of CD and DD. Cows with CC genotype had a significant increase in the total number of ova (TNO) (P

Published
2009

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