1. DNA methylation changes in cell line from β-lactoglobulin gene targeted fetus
- Author
-
Li Lin, Weizhuo Xu, Ning Li, and Yunping Dai
- Subjects
Vascular Endothelial Growth Factor A ,RNA, Untranslated ,Lactoglobulins ,Biology ,Cell Line ,Animals, Genetically Modified ,Fetus ,Endocrinology ,Food Animals ,Insulin-Like Growth Factor II ,Gene expression ,Animals ,Epigenetics ,Telomerase ,Gene ,RNA-Directed DNA Methylation ,Epigenomics ,ADP Ribose Transferases ,Gene Expression Regulation, Developmental ,Gene targeting ,General Medicine ,Methylation ,DNA Methylation ,Molecular biology ,Gene Targeting ,DNA methylation ,Cattle ,CpG Islands ,RNA, Long Noncoding ,Animal Science and Zoology ,Octamer Transcription Factor-3 - Abstract
The gene targeting combined somatic cell nuclear transfer is very useful in agriculture and medicine. Epigenetic modification of DNA by methylation is significant in regulating gene expression during mammalian development. During gene targeting, epigenetic status of donor cell nuclei may be changed in a series of processes, including homologous recombination, cell selection and cloning. We examined DNA methylation of six genes ( β-actin , VEGF , oct4 , TERT , H19 and Igf2 ) and a repetitive sequence art 2 in blg +/− cell line from β-lactoglobulin (BLG) gene targeted fetus and the cells used for BLG gene targeting serve as control. The results demonstrated that the widespread changes of DNA methylation were found in blg +/− cell line. But the degree of variation was different. DNA methylation of VEGF in blg +/− was noticeably decreased. These observations suggest that DNA methylation variations may impact gene expression and finally induce abnormalities and lethality in later developmental stages.
- Published
- 2009
- Full Text
- View/download PDF