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In avian species, the surgical technique for ovarian allotransplantation has been developed for domestic chickens; however, not all genotypes can be effectively used as recipients. The aims of the present study were to ascertain donor/recipient combinations for production of offspring from frozen/thawed ovarian tissues. The development of the technique is important because domestic chicken offspring have only been produced from fresh (never frozen) ovarian and from frozen-thawed testicular tissues. Information obtained from evaluating genetic differences of intensively selected lines in which there was successful pairing was compared in the indigenous breeds. Results indicate donor/recipient combinations were created which could be effectively used for gonadal tissue allotransplantations. Gonadal tissues of Yellow, Speckled and Partridge-color Hungarian, Black and Speckled Transylvanian Naked Neck chicken breeds were allotransplanted into White Leghorn or Novogen White breeds for offspring production. The gonadal tissues of these indigenous breeds were cryopreserved using vitrification procedures. There was successful allografting of frozen/thawed gonadal tissues at a rate between 20 % and 100 % depending on the genotype and sex, and histological examination and microsatellite marker analysis provided evidence that the donor ovarian and testicular tissues had the capacity for producing gametes. The hens of Speckled Transylvanian Naked Neck/White Leghorn combination using frozen/thawed ovarian tissues were produced for progeny tests. Of these, 58 % produced eggs and 9.1 % produced donor-derived offspring, based on data for both feather color markers and genetic analysis.

The aim of the study was to devise a technique to allow transfer of ovarian and testicular tissues obtained from one day old chicks to recipient animals. The following combinations of chicken breeds were used: Tetra SL/Tetra SL, Tetra SL/Harco, Tetra SL/Black Transylvanian Naked Neck, Godollo New Hampshire/Speckled Transylvanian Naked Neck (recipient/donor), respectively. Only animals less than 24 h old from hatching were used as either recipient or donor. Eggs yielding recipients were treated with busulfan to hinder the development of the recipient's own gonads. Gonads were transferred surgically to the new host which was then immunosuppressed for two months. The animals were checked at 8 or 16 weeks for the existence of implanted gonads, and if found, the gonads were removed for histology examination. Tetra SL/Tetra SL pairing resulted in successfully adhered and functioning gonads in most cases. In other combinations, no gonads originating from the donors were found in the recipients. We conclude that not all breeds seem to be suitable recipients; some breeds or individuals may show incompatibility to each other and further examination is needed to find the cause of incompatibility and to establish a suitable breed combination, which can be used for gonad transfer.