1. An international parentage and identification panel for the domestic cat (Felis catus).
- Author
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Lipinski, MJ, Amigues, Y, Blasi, M, Broad, TE, Cherbonnel, C, Cho, GJ, Corley, S, Daftari, P, Delattre, DR, Dileanis, S, Flynn, JM, Grattapaglia, D, Guthrie, A, Harper, C, Karttunen, PL, Kimura, H, Lewis, GM, Longeri, M, Meriaux, J-C, Morita, M, Morrin-O'donnell, RC, Niini, T, Pedersen, NC, Perrotta, G, Polli, M, Rittler, S, Schubbert, R, Strillacci, MG, Van Haeringen, H, Van Haeringen, W, and Lyons, LA
- Subjects
Animals ,Cats ,Genetic Markers ,Microsatellite Repeats ,Genotype ,Polymorphism ,Genetic ,Alleles ,cat ,feline ,identification ,microsatellite ,parentage ,Polymorphism ,Genetic ,Dairy & Animal Science ,Genetics ,Zoology ,Veterinary Sciences - Abstract
Seventeen commercial and research laboratories participated in two comparison tests under the auspices of the International Society for Animal Genetics to develop an internationally tested, microsatellite-based parentage and identification panel for the domestic cat (Felis catus). Genetic marker selection was based on the polymorphism information content and allele ranges from seven random-bred populations (n = 261) from the USA, Europe and Brazil and eight breeds (n = 200) from the USA. Nineteen microsatellite markers were included in the comparison test and genotyped across the samples. Based on robustness and efficiency, nine autosomal microsatellite markers were ultimately selected as a single multiplex 'core' panel for cat identification and parentage testing. Most markers contained dinucleotide repeats. In addition to the autosomal markers, the panel included two gender-specific markers, amelogenin and zinc-finger XY, which produced genotypes for both the X and Y chromosomes. This international cat parentage and identification panel has a power of exclusion comparable to panels used in other species, ranging from 90.08% to 99.79% across breeds and 99.47% to 99.87% in random-bred cat populations.
- Published
- 2007