1. The effect of curcumin on insulin release in rat-isolated pancreatic islets.
- Author
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Abdel Aziz MT, El-Asmar MF, El Nadi EG, Wassef MA, Ahmed HH, Rashed LA, Obaia EM, Sabry D, Hassouna AA, and Abdel Aziz AT
- Subjects
- Animals, DNA Primers, Electrophoresis, Agar Gel, Enzyme-Linked Immunosorbent Assay, Gene Expression, Glucose pharmacology, Heme Oxygenase-1 genetics, Hemin pharmacology, Islets of Langerhans metabolism, Metalloporphyrins pharmacology, Rats, Reverse Transcriptase Polymerase Chain Reaction, Curcumin pharmacology, Heme Oxygenase-1 metabolism, Insulin metabolism, Islets of Langerhans drug effects
- Abstract
Curcumin exerts a hypoglycemic action and induces heme-oxygenase-1 (HO-1). We evaluated the effect of curcumin on isolated islets of Langerhans and studied whether its action on insulin secretion is mediated by inducible HO-1. Islets were isolated from rats and divided into control islets, islets incubated in different curcumin concentrations, islets incubated in hemin, islets incubated in curcumin and HO inhibitor, stannous mesoporphyrin (SnMP), islets incubated in hemin and SnMP, islets incubated in SnMP only, and islets incubated in 16.7 mmol/L glucose. Heme-oxygenase activity, HO-1 expression, and insulin estimation was assessed. Insulin secretion, HO-1 gene expression and HO activity were significantly increased in islets incubated in curcumin, hemin, and glucose compared with controls. This increase in insulin secretion was significantly decreased by incubation of islets in SnMP. The action of curcumin on insulin secretion from the isolated islets may be, in part, mediated through increased HO-1 gene expression.
- Published
- 2010
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