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Your search keyword '"Zuo, Xiaolei"' showing total 16 results
Start Over Author "Zuo, Xiaolei" Publication Year Range Last 50 years Journal angewandte chemie international edition
16 results on '"Zuo, Xiaolei"'

1. A Programmable DNAzyme for the Sensitive Detection of Nucleic Acids

Author

Shi, Chenzhi, primary, Yang, Donglei, additional, Ma, Xiaowei, additional, Pan, Li, additional, Shao, Yuanchuan, additional, Arya, Gaurav, additional, Ke, Yonggang, additional, Zhang, Chuan, additional, Wang, Fuan, additional, Zuo, Xiaolei, additional, Li, Min, additional, and Wang, Pengfei, additional

Published
2024
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2. Directing the Encapsulation of Single Cells with DNA Framework Nucleator‐Based Hydrogel Growth.

Author

Wei, Yuhan, Feng, Yueyue, Wang, Kaizhe, Wei, Yuhui, Li, Qian, Zuo, Xiaolei, Li, Bin, Li, Jiang, Wang, Lihua, Fan, Chunhai, and Zhu, Ying

Subjects
DNA structure, DNA, BIOMIMETIC materials, CELL culture, STRUCTURAL frames, SINGLE-stranded DNA
Abstract

Encapsulating individual mammalian cells with biomimetic materials holds potential in ex vivo cell culture and engineering. However, current methodologies often present tradeoffs between homogeneity, stability, and cell compatibility. Here, inspired by bacteria that use proteins stably anchored on their outer membranes to nucleate biofilm growth, we develop a single‐cell encapsulation strategy by using a DNA framework structure as a nucleator (DFN) to initiate the growth of DNA hydrogels under cell‐friendly conditions. We find that among the tested structures, the tetrahedral DFN can evenly and stably reside on cell membranes, effectively initiating hybridization chain reactions which generate homogeneously dense yet flexible single‐cell encapsulation for diverse cell lines. The encapsulation persists for up to 72 hours in a serum‐containing cell culture environment, representing a ~70‐fold improvement compared to encapsulations mediated by single‐stranded DNA nucleators. The metabolism and proliferation of the encapsulated cells are suppressed, but can be restored to the original efficiencies upon release, suggesting the superior cell compatibility of the encapsulation. We also find that compared to naked cells, the encapsulated cells exhibit a lower autophagy level after undergoing mechanical stress, suggesting the protective effect of the DNA encapsulation. This method may provide a new tool for ex vivo cell engineering. [ABSTRACT FROM AUTHOR]

Published
2024
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5. A Membrane Tension‐Responsive Mechanosensitive DNA Nanomachine.

Author

Zheng, Haoran, Li, Haidong, Li, Mingqiang, Zhai, Tingting, Xie, Xiaodong, Li, Cong, Jing, Xinxin, Liang, Chengpin, Li, Qian, Zuo, Xiaolei, Li, Jiang, Fan, Jiangli, Shen, Jianlei, Peng, Xiaojun, and Fan, Chunhai

Subjects
ION channels, DNA, MEMBRANE lipids, ISOMERS, DNA nanotechnology, ISOMERIZATION
Abstract

Membrane curvature reflects physical forces operating on the lipid membrane, which plays important roles in cellular processes. Here, we design a mechanosensitive DNA (MSD) nanomachine that mimics natural mechanosensitive PIEZO channels to convert the membrane tension changes of lipid vesicles with different sizes into fluorescence signals in real time. The MSD nanomachine consists of an archetypical six‐helix‐bundle DNA nanopore, cholesterol‐based membrane anchors, and a solvatochromic fluorophore, spiropyran (SP). We find that the DNA nanopore effectively amplifies subtle variations of the membrane tension, which effectively induces the isomerization of weakly emissive SP into highly emissive merocyanine isomers for visualizing membrane tension changes. By measuring the membrane tension via the fluorescence of MSD nanomachine, we establish the correlation between the membrane tension and the curvature that follows the Young‐Laplace equation. This DNA nanotechnology‐enabled strategy opens new routes to studying membrane mechanics in physiological and pathological settings. [ABSTRACT FROM AUTHOR]

Published
2023
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6. DNA‐Encoded Gold‐Gold Wettability for Programmable Plasmonic Engineering

Author

Zhai, Tingting, primary, Zheng, Haoran, additional, Fang, Weina, additional, Gao, Zhaoshuai, additional, Song, Shiping, additional, Zuo, Xiaolei, additional, Li, Qian, additional, Wang, Lihua, additional, Li, Jiang, additional, Shi, Jiye, additional, Liu, Xiaoguo, additional, Tian, Yang, additional, Shen, Jianlei, additional, and Fan, Chunhai, additional

Published
2022
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9. DNA Framework‐Based Topological Cell Sorters

Author

Yin, Fangfei, primary, Li, Min, additional, Mao, Xiuhai, additional, Li, Fan, additional, Xiang, Xuelin, additional, Li, Qian, additional, Wang, Lihua, additional, Zuo, Xiaolei, additional, Fan, Chunhai, additional, and Zhu, Ying, additional

Published
2020
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12. Valency-Controlled Framework Nucleic Acid Signal Amplifiers

Author

Liu, Qi, primary, Ge, Zhilei, additional, Mao, Xiuhai, additional, Zhou, Guobao, additional, Zuo, Xiaolei, additional, Shen, Juwen, additional, Shi, Jiye, additional, Li, Jiang, additional, Wang, Lihua, additional, Chen, Xiaoqing, additional, and Fan, Chunhai, additional

Published
2018
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14. An Exonuclease III‐Powered, On‐Particle Stochastic DNA Walker

Author

Qu, Xiangmeng, primary, Zhu, Dan, additional, Yao, Guangbao, additional, Su, Shao, additional, Chao, Jie, additional, Liu, Huajie, additional, Zuo, Xiaolei, additional, Wang, Lihua, additional, Shi, Jiye, additional, Wang, Lianhui, additional, Huang, Wei, additional, Pei, Hao, additional, and Fan, Chunhai, additional

Published
2017
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16. Programmable Engineering of a Biosensing Interface with Tetrahedral DNA Nanostructures for Ultrasensitive DNA Detection.

Author

Lin, Meihua, Wang, Jingjing, Wu, Na, Zuo, Xiaolei, Fan, Chunhai, Zhou, Guobao, Wang, Jianbang, Shi, Jiye, Chen, Xiaoqing, Lu, Jianxin, and Gao, Jimin

Subjects
NANOSTRUCTURES, DNA analysis, SOFT lithography, TETRAHEDRAL molecules, NUCLEIC acid hybridization, FLUORESCENCE resonance energy transfer
Abstract

Self-assembled DNA nanostructures with precise sizes allow a programmable 'soft lithography' approach to engineer the interface of electrochemical DNA sensors. By using millimeter-sized gold electrodes modified with several types of tetrahedral DNA nanostructures (TDNs) of different sizes, both the kinetics and thermodynamics of DNA hybridization were profoundly affected. Because each DNA probe is anchored on an individual TDN, its lateral spacing and interactions are finely tuned by the TDN size. By simply varying the size of the TDNs, the hybridization time was decreased and the hybridization efficiency was increased. More significantly, the detection limit for DNA detection was tuned over four orders of magnitude with differentially nanostructured electrodes, and achieved attomolar sensitivity with polymeric enzyme amplification. [ABSTRACT FROM AUTHOR]

Published
2015
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