1. Development of α-Gal-Antibody Conjugates to Increase Immune Response by Recruiting Natural Antibodies.
- Author
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Sianturi J, Manabe Y, Li HS, Chiu LT, Chang TC, Tokunaga K, Kabayama K, Tanemura M, Takamatsu S, Miyoshi E, Hung SC, and Fukase K
- Subjects
- Antibodies chemistry, Carbohydrate Conformation, Cell Line, Tumor, Cell Survival immunology, Humans, Neoplasms pathology, Neoplasms therapy, Trisaccharides chemical synthesis, Trisaccharides chemistry, Antibodies immunology, Neoplasms immunology, Trisaccharides immunology
- Abstract
Cancer treatment with antibodies (Abs) is one of the most successful therapeutic strategies for obtaining high selectivity. In this study, α-gal-Ab conjugates were developed that dramatically increased cellular cytotoxicity by recruiting natural Abs through the interaction between α-gal and anti-gal Abs. The potency of the α-gal-Ab conjugates depended on the amount of α-gal conjugated to the antibody: the larger the amount of α-gal introduced, the higher the level of cytotoxicity observed. The conjugation of antibodies with an α-gal dendrimer allowed the introduction of large amounts of α-gal to the Ab, without loss of affinity for the target cell. The method described here will enable the re-development of Abs to improve their potency., (© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2019
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