1. Expanding the Hydrophobic Cavity Surface of Azocalix[4]arene to Enable Biotin/Avidin Affinity with Controlled Release.
- Author
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Chen FY, Li CZ, Han H, Geng WC, Zhang SX, Jiang ZT, Zhao QY, Cai K, and Guo DS
- Subjects
- Humans, Surface Properties, Doxorubicin chemistry, Doxorubicin pharmacology, Doxorubicin metabolism, Delayed-Action Preparations chemistry, Phenols chemistry, Calixarenes chemistry, Biotin chemistry, Hydrophobic and Hydrophilic Interactions, Avidin chemistry, Avidin metabolism
- Abstract
The development of artificial receptors that combine ultrahigh-affinity binding and controllable release for active guests holds significant importance in biomedical applications. On one hand, a complex with an exceedingly high binding affinity can resist unwanted dissociation induced by dilution effect and complex interferents within physiological environments. On the other hand, stimulus-responsive release of the guest is essential for precisely activating its function. In this context, we expanded hydrophobic cavity surface of a hypoxia-responsive azocalix[4]arene, affording Naph-SAC4A. This modification significantly enhanced its aqueous binding affinity to 10
13 M-1 , akin to the naturally occurring strongest recognition pair, biotin/(strept-)avidin. Consequently, Naph-SAC4A emerges as the first artificial receptor to simultaneously integrate ultrahigh recognition affinity and actively controllable release. The markedly enhanced affinity not only improved Naph-SAC4A's sensitivity in detecting rocuronium bromide in serum, but also refined the precision of hypoxia-responsive doxorubicin delivery at the cellular level, demonstrating its immense potential for diverse practical applications., (© 2024 Wiley-VCH GmbH.)- Published
- 2024
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