1. Real-Time In Vivo Detection of Cellular Senescence through the Controlled Release of the NIR Fluorescent Dye Nile Blue
- Author
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Ramón Martínez-Máñez, Alba García-Fernández, Beatriz Lozano-Torres, Irene Galiana, Juan F. Blandez, Félix Sancenón, María Alfonso, Mar Orzáez, and María Dolores Marcos
- Subjects
Senescence ,senescence ,010402 general chemistry ,01 natural sciences ,Mesoporous nanoparticles ,Catalysis ,chemistry.chemical_compound ,Mice ,QUIMICA ORGANICA ,in vivo detection ,In vivo ,Oxazines ,BIOQUIMICA Y BIOLOGIA MOLECULAR ,medicine ,Animals ,Humans ,Cellular Senescence ,Fluorescent Dyes ,010405 organic chemistry ,Chemistry ,Melanoma ,QUIMICA INORGANICA ,Nile blue ,General Medicine ,General Chemistry ,Mesoporous silica ,medicine.disease ,Controlled release ,Fluorescence ,In vitro ,0104 chemical sciences ,NIR probes ,In vivo detection ,Biophysics ,Female ,mesoporous nanoparticles - Abstract
[EN] In vivo detection of cellular senescence is accomplished by using mesoporous silica nanoparticles loaded with the NIR-FDA approved Nile blue (NB) dye and capped with a galactohexasaccharide (S3). NB emission at 672 nm is highly quenched inside S3, yet a remarkable emission enhancement is observed upon cap hydrolysis in the presence of beta-galactosidase and dye release. The efficacy of the probe to detect cellular senescence is tested in vitro in melanoma SK-Mel-103 and breast cancer 4T1 cells and in vivo in palbociclib-treated BALB/cByJ mice bearing breast cancer tumor., R.M. thanks financial support from the Spanish Government (RTI2018-100910-B-C41 and RTI2018-101599-B-C22 (MCUI/AEI/FEDER, UE)) and the Generalitat Valenciana (PROMETEO 2018/024). M.O. thanks the financial support from SAF2017-84689-R project and MINECO/AEI/FEDER, UE and the Generalitat Valenciana (PROMETEO/2019/065). B.L.-T. is grateful to the Spanish Ministry of Economy for her PhD grant. I.G. thanks her contract from IDM. J.F.-B. and M.A. thank the UPV for their postdoctoral fellowship.
- Published
- 2020