1. Relation of nitric oxide synthase gene (NOS3) polymorphisms to varicocele risk and post-varicocelectomy seminal oxidative stress reduction.
- Author
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Omar SS, Mahfouz W, Dawood W, Abo El-Wafa RAH, Ghazala RA, and Zahran AM
- Subjects
- Adult, Case-Control Studies, Genetic Predisposition to Disease, Humans, Incidence, Infertility, Male genetics, Infertility, Male pathology, Male, Malondialdehyde analysis, Malondialdehyde metabolism, Middle Aged, Oxidative Stress genetics, Polymorphism, Single Nucleotide, Prognosis, Semen metabolism, Spermatic Cord blood supply, Spermatic Cord surgery, Treatment Outcome, Varicocele epidemiology, Varicocele surgery, Infertility, Male prevention & control, Nitric Oxide Synthase Type III genetics, Urologic Surgical Procedures, Male, Varicocele genetics, Vascular Surgical Procedures
- Abstract
The pathophysiology of varicocele remains to be unknown. Several genetic factors have been implicated in varicocele etiopathogenesis. We studied the relationship between NOS3 c.894G>T, c.786T>C and 4b/a polymorphisms to varicocele risk and their prognostic value as regards improvement of the post-operative seminal parameters &/or seminal malonaldehyde levels. The three NOS3 polymorphisms were evaluated in 100 patients with varicocele and 100 healthy subjects by RT-PCR. Seminal plasma MDA level was measured pre-operatively and 3 months after varicocelectomy by the thiobarbituric acid method. The GT, TT, TC and bb genotypes of NOS3 polymorphism were more commonly observed in varicocele patients (30%, 9%, 28% and 70% respectively) compared to normal controls (12%, 0%, 10% and 50% respectively). The mean percentage of post-varicocelectomy seminal MDA reduction was highest with the GT genotype (p < .001). Genotypes GT+TT, TC and bb were associated with varicocele occurrence in our patients. The T (c.894G>T), C (c.786T>C) and b (NOS3 intron 4 VNTR) alleles were significantly associated with varicocele occurrence in our cohort of patients. We also report a better response regarding the reduction of seminal MDA after varicocelectomy with the GT and ba genotypes., (© 2020 Blackwell Verlag GmbH.)
- Published
- 2020
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