Search

Your search keyword '"Yu. Shevelev"' showing total 1 results
Start Over Author "Yu. Shevelev" Journal analytical methods
1 results on '"Yu. Shevelev"'

1. Effect of the relief on the measurement of bond rupture force with the help of AFM: the dynamics of interaction and optimization of the procedure

Author

Alexander A. Lomzov, F. N. Dultsev, Dmitrii V. Pyshnyi, Nina N. Kurus, and G. Yu. Shevelev

Subjects
Materials science, 010405 organic chemistry, Atomic force microscopy, General Chemical Engineering, Dynamics (mechanics), General Engineering, Mode (statistics), 02 engineering and technology, 021001 nanoscience & nanotechnology, 01 natural sciences, Bond rupture, 0104 chemical sciences, Analytical Chemistry, Helix, Surface roughness, Surface modification, Wafer, Composite material, 0210 nano-technology
Abstract

Measurement of the forces of unwinding of DNA double helix was conducted. Silicon wafers were chosen as substrates. It is demonstrated that the use of gold for surface modification causes the bimodal probability distribution of rupture forces because of the surface roughness. Surface modification with the help of Langmuir–Blodgett procedure is proposed as an alternative. The use of this method gives unimodal distribution of rupture forces. The forces of double helix unwinding are reported for oligonucleotides containing 20 base pairs depending on salt concentration. Furthermore, forces for the shear mode and unzipping mode are measured.

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results