1. Rapid and sensitive chromatographic determination of free sialic acid in complex bio-pharma fermentation media samples
- Author
-
Brett Paull, Damian Connolly, and Hassan Alwael
- Subjects
Peak area ,Monolithic HPLC column ,Chromatography ,Thiobarbituric acid ,General Chemical Engineering ,General Engineering ,Periodic acid ,Quantitative determination ,Analytical Chemistry ,Sialic acid ,chemistry.chemical_compound ,chemistry ,Fermentation ,Retention time - Abstract
This paper describes a rapid, sensitive and reproducible liquid chromatographic method specifically for the quantitative determination of total free sialic acid, employing thiobarbituric acid as pre-column tagging agent, following the oxidation of sialic acid with periodic acid. The derivatised sialic acid was separated from 2-deoxy-D-ribose and the other components utilising a short C18 monolithic column, with total run-times of under 90 s per sample. The method was successfully applied to quantify sialic acid in a range of complex samples, e.g. yeastolate powders, basal media and in-process samples supplied from the biopharmaceutical industry. The selective method was highly reproducible (%RSD for method (n = 6) =
- Published
- 2012