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A highly sensitive and simple method for determining cryptotanshinone (Cry), tanshinone I (Tan I), and tanshinone IIA (Tan IIA) in Salvia miltiorrhiza was developed using micro HPLC with electrochemical detection (µHPLC-ED). The tanshinones were extracted using an ultrasonic method, with methanol as the extractant. The separation was carried out on a C18 (150 mm × 1.0 mm i.d., 3 µm particle size) analytical column. The mobile phase was acetonitrile-water-formic acid mixture (52:48:0.6, v/v/v) solution. The flow rate and the temperature of the column were set at 30 µL/min and 35°C. The applied potential was set at −0.4 V vs. Ag/AgCl. The peak heights for Cry, Tan I, and Tan IIA were found to be linearly related to the amounts injected, ranging from 0.145 µmol/L to 3.88 µmol/L, 0.226 µmol/L to 3.01 µmol/L, and 0.149 µmol/L to 3.98 µmol/L, respectively. The RSD values of intra-day precision and repeatability were not more than 3.0%, and the recovery of three analytes were ranged from 96.7% to 97.5%. The resu...

The capcity factors determined by reversed-phase micro high performance liquid chromatography (micro-HPLC) can be used as one of the descriptors in Quantitative-Structure-Activity-Relationships (QSAR) studies. The present method gives a more simple and convenient way to study relationships between physicochemical properties and biological activities of some drugs.

The combination technique of micro high performance liquid chromatography and inductively coupled plasma atomic emission spectrometry is demonstrated in the separation of organometallic compounds. Problems relating to the interfacing between micro HPLC and ICP would be elininated uti1izing the simple interfacing technique.

The chromatographic potential of very short fused-silica column (30 mm length) packed with 3-μm particles has been investigated. The results indicate that this kind of micro-column has high applicability for a rapid identification of several samples. This technique also meets the recent demands in HPLC, as low solvent consumption and faster analysis time.