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Your search keyword '"Zhao, Xinfeng"' showing total 6 results
Start Over Author "Zhao, Xinfeng" Journal analytical chemistry
6 results on '"Zhao, Xinfeng"'

5. Reliable Analysis of the Interaction between Specific Ligands and Immobilized Beta-2-Adrenoceptor by Adsorption Energy Distribution

Author

Li, Qian, primary, Ning, Xiaohui, additional, An, Yuxin, additional, Stanley, Brett J., additional, Liang, Yuan, additional, Wang, Jing, additional, Zeng, Kaizhu, additional, Fei, Fuhuan, additional, Liu, Ting, additional, Sun, Huanmei, additional, Liu, Jiajun, additional, Zhao, Xinfeng, additional, and Zheng, Xiaohui, additional

Published
2018
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6. Site-Specific Immobilization of β 2 -AR Using O 6 -Benzylguanine Derivative-Functionalized Supporter for High-Throughput Receptor-Targeting Lead Discovery.

Author

Wang J, Wang Y, Liu J, Li Q, Yin G, Zhang Y, Xiao C, Fan T, Zhao X, and Zheng X

Subjects
Cinnamates chemistry, Depsides chemistry, Guanine chemistry, Guanine metabolism, Humans, Ligands, Perilla chemistry, Receptors, Adrenergic, beta-2 analysis, Surface Properties, Rosmarinic Acid, Cinnamates pharmacology, Depsides pharmacology, Drug Discovery, Guanine analogs & derivatives, High-Throughput Screening Assays, Receptors, Adrenergic, beta-2 metabolism
Abstract

The past decade has witnessed the great promise of strategies for ligand discovery based on surface-immobilized GPCRs. We present here a method for preparation of immobilized GPCRs. Key features include covalent immobilization with high specificity and robust application in drug-receptor interaction analysis and ligand screening. In our example assay using beta 2 -adrenergic receptor (β 2 -AR), the human DNA repair protein O 6 -alkylguanine-DNA alkyltransferase (hAGT) fusion receptor expressed in Escherichia coli was directly captured onto polyethylene glycol polyacrylamide (PEGA) resin. We observed even distribution and physiological functions of β 2 -AR on the resin. The immobilized β 2 -AR as a stationary phase enabled us to rapidly determine the binding of four drugs to β 2 -AR. By coupling this assay to mass spectrometry, we screened rosmarinic acid as a bioactive compound targeting β 2 -AR in Fructus Perillae. We concluded that O 6 -benzylguanine derivative-functionalized supporter is promising for specific immobilization of hAGT-tagged proteins; immobilized receptor chromatography has great potential in screening receptor-binding leads from herbal plants or traditional medicine recipes.

Published
2019
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