1. Molecular logic gates on DNA origami nanostructures for microRNA diagnostics
- Author
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Shiping Song, Huajie Liu, Bin Dai, Jie Chao, Bin Zhao, Dongfang Wang, Dannong He, Chunhai Fan, Juan Yan, Yi Zhang, and Yanming Fu
- Subjects
Nanostructure ,business.industry ,Chemistry ,Computation ,Nanotechnology ,DNA ,Analytical Chemistry ,Nanostructures ,Computers, Molecular ,MicroRNAs ,Molecular level ,Logic gate ,DNA origami ,business ,Computer hardware ,Hardware_LOGICDESIGN - Abstract
Molecular computing holds great promise for diagnosis and treatment of diseases at the molecular level; nevertheless, designing molecular logic gates to operate programmably and autonomously for molecular diagnostics still remains challenging. We designed logic gates on DNA Origami for microRNA analysis. As a demonstration, two indicators of heart failure, microRNA-21 and microRNA-195, were selected as the logic inputs. The logic gates contain two main modules: computation module and output module, performing in a single DNA Origami nanostructure. The computation module recognizes disease indicators, while the output module display different nanoscale symbols, “+” (positive) or “–” (negative), depending on the computing results. We demonstrated that the molecular logic gates worked well with single and two input combinations.
- Published
- 2014