1. Single Chain Cationic Polymer Dot as a Fluorescent Probe for Cell Imaging and Selective Determination of Hepatocellular Carcinoma Cells
- Author
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Serdar Özçelik, Hakan Kaya, Özge Tüncel, Umit Hakan Yildiz, Sezer Özenler, Muge Yucel, Özenler, Sezer, Yücel, Müge, Tüncel, Özge, Kaya, Hakan, Özçelik, Serdar, Yıldız, Ümit Hakan, Izmir Institute of Technology. Chemistry, Izmir Institute of Technology. Biotechnology and Bioengineering, and Izmir Institute of Technology. Photonics
- Subjects
Dispersity ,Thiophenes ,010402 general chemistry ,01 natural sciences ,Piperazines ,Analytical Chemistry ,chemistry.chemical_compound ,Colloid ,Bromide ,Cations ,Cell Line, Tumor ,Quantum Dots ,Humans ,Particle Size ,Cell Line, Transformed ,Fluorescent Dyes ,Cell Nucleus ,Staining and Labeling ,010401 analytical chemistry ,Optical Imaging ,Cationic polymerization ,Fluorescence ,Polyelectrolytes ,Coculture Techniques ,0104 chemical sciences ,chemistry ,Cancer cell ,Biophysics ,Hepatocytes ,Luminescence ,Ethylene glycol - Abstract
PubMed: 31334629, This letter describes formation of single chain cationic polymer dots (Pdots) made of poly[1,4-dimethy1-1-(34(2,4,5-trimethylthiophen-3-yl)oxy)propyl)piperazin-1-ium bromide] conjugated polyelectrolyte (CPE). The single chain Pdot formation relies on a simple process which is a rapid nanophase separation between CPE solution of ethylene glycol and water. Pdots show narrow monodisperse size distribution with a 3.6 nm in diameter exhibiting high brightness and excellent colloidal and optical stability. It has been demonstrated that photoluminescent Pdots provide selective nuclear translocation to hepatocellular carcinoma cells as compared to healthy liver cells. The Pdot labeling effectively discriminates cancer cells in the coculture media. Pdots hold great promise as a luminescent probe to diagnose cancer cells in histology and may guide surgeons during operations to precisely separate out cancerous tissue due to augmented fluorescence brightness.
- Published
- 2019