1. Flow Cytometry-Based Quantification of Cellular Au Nanoparticles
- Author
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Jonghoon Park, Nuri Yang, Tae Hyun Yoon, and My Kieu Ha
- Subjects
inorganic chemicals ,education ,Analytical chemistry ,Nanoparticle ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Analytical Chemistry ,Flow cytometry ,HeLa ,mental disorders ,Cervical carcinoma ,medicine ,Surface charge ,Inductively coupled plasma mass spectrometry ,health care economics and organizations ,Cell specific ,medicine.diagnostic_test ,biology ,Chemistry ,technology, industry, and agriculture ,021001 nanoscience & nanotechnology ,biology.organism_classification ,0104 chemical sciences ,0210 nano-technology - Abstract
There has been a great deal of research regarding the cellular association of nanoparticles (NPs), although there are only a few methods available yet for the quantitative measurements of cellular NPs. In this study, we propose a simple and quantitative method to estimate the cellular uptake of Au NPs into cervical cancer cells (HeLa) based on their side scattering (SSC) intensities measured by flow cytometry (FCM). We have compared SSC intensities of HeLa cells exposed to eight different types of Au NPs (40-100 nm size, with positive or negative surface charge) with the amount of cellular Au NPs measured by inductively coupled plasma mass spectrometry (ICPMS). On the basis of these comparisons, we have found linear correlations between the cellular Au NPs and the SSC intensities and used them to estimate the amount of Au NPs associated with HeLa cells. Once the correlations were found for specific cell lines and types of nanoparticles, this approach is useful for simple and quantitative estimation of the cellular Au NPs, without performing labor-intensive and complicated sample preparation procedures required for the ICPMS approach.
- Published
- 2017