1. Ratiometric two-photon fluorescent probe for quantitative detection of β-galactosidase activity in senescent cells
- Author
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Hae-Ok Byun, Hyo Won Lee, Hwan Myung Kim, Gyesoon Yoon, In Hae Kwak, Cheol Ho Heo, and Debabrata Sen
- Subjects
In situ ,chemistry.chemical_classification ,Reactive oxygen species ,Chemistry ,Cellular senescence ,Galactosidase activity ,beta-Galactosidase ,Molecular biology ,Fluorescence ,Analytical Chemistry ,Two-photon excitation microscopy ,Biophysics ,Cytotoxicity ,Radiometry ,Cell aging ,Cellular Senescence ,Fluorescent Dyes - Abstract
We reported a ratiometric two-photon fluorescent probe (SG1) for β-galactosidase (β-gal) and its application to quantitative detection of β-gal activity during cellular senescence in live cells and in aged tissues. This probe is characterized by a significant two-photon excited fluorescence, a marked blue-to-yellow emission color change in response to β-gal, easy loading, insensitivity to pH and reactive oxygen species (ROS), high photostability, and low cytotoxicity. In addition, we show that SG1 labeling is an effective tool for quantitative detection of senescence-associated β-gal activity at the subcellular level in situ. This finding demonstrates that SG1 will find useful applications in biomedical research, including studies of cell aging.
- Published
- 2014