11 results on '"Can Qian"'
Search Results
2. Dual-Modal Apoptosis Assay Enabling Dynamic Visualization of ATP and Reactive Oxygen Species in Living Cells
- Author
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Lei Jiang, Hua-Ying Chen, Cai-Hong He, Han-Bin Xu, Ze-Rui Zhou, Man-Sha Wu, Essy Kouadio Fodjo, Yue He, Mahmoud Elsayed Hafez, Ruo-Can Qian, and Da-Wei Li
- Subjects
Analytical Chemistry - Published
- 2023
3. Specially Resolved Single Living Cell Perfusion and Targeted Fluorescence Labeling Based on Nanopipettes
- Author
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Jian Lv, Xiao-Yuan Wang, Xin-Yue Zhou, Da-Wei Li, and Ruo-Can Qian
- Subjects
Perfusion ,Isothiocyanates ,Nanotechnology ,DNA ,Fluoresceins ,Fluorescent Dyes ,Analytical Chemistry - Abstract
Targeted delivery and labeling of single living cells in heterogeneous cell populations are of great importance to understand the molecular biology and physiological functions of individual cells. However, it remains challenging to perfuse fluorescence markers into single living cells with high spatial and temporal resolution without interfering neighboring cells. Here, we report a single cell perfusion and fluorescence labeling strategy based on nanoscale glass nanopipettes. With the nanoscale tip hole of 100 nm, the use of nanopipettes allows special perfusion and high-resolution fluorescence labeling of different subcellular regions in single cells of interest. The dynamic of various fluorescent probes has been studied to exemplify the feasibility of nanopipette-dependent targeted delivery. According to experimental results, the cytoplasm labeling of Sulfo-Cyanine5 and fluorescein isothiocyanate is mainly based on the Brownian movement due to the dyes themselves and does not have a targeting ability, while the nucleus labeling of 4',6-diamidino-2-phenylindole (DAPI) is originated from the adsorption between DAPI and DNA in the nucleus. From the finite element simulation, the precise manipulation of intracellular delivery is realized by controlling the electro-osmotic flow inside the nanopipettes, and the different delivery modes between nontargeting dyes and nucleus-targeting dyes were compared, showcasing the valuable ability of nanopipette-based method for the analysis of specially defined subcellular regions and the potential applications for single cell surgery, subcellular manipulation, and gene delivery.
- Published
- 2022
4. Dynamic Visualization of Endoplasmic Reticulum Stress in Living Cells via a Two-Stage Cascade Recognition Process
- Author
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Man-Sha Wu, Ze-Rui Zhou, Xiao-Yuan Wang, Bin-Bin Chen, Mahmoud Elsayed Hafez, Ji-Fen Shi, Da-Wei Li, and Ruo-Can Qian
- Subjects
Autophagy ,Endoplasmic Reticulum ,Endoplasmic Reticulum Stress ,Fluorescent Dyes ,Analytical Chemistry - Abstract
The endoplasmic reticulum (ER) is crucial for the regulation of multiple cellular processes, such as cellular responses to stress and protein synthesis, folding, and posttranslational modification. Nevertheless, monitoring ER physiological activity remains challenging due to the lack of powerful detection methods. Herein, we built a two-stage cascade recognition process to achieve dynamic visualization of ER stress in living cells based on a fluorescent carbon dot (CD) probe, which is synthesized by a facile one-pot hydrothermal method without additional modification. The fluorescent CD probe enables two-stage cascade ER recognition by first accumulating in the ER as the positively charged and lipophilic surface of the CD probe allows its fast crossing of multiple membrane barriers. Next, the CD probe can specifically anchor on the ER membrane via recognition between boronic acids and
- Published
- 2022
5. Nanopipette-Based Nanosensor for Label-Free Electrochemical Monitoring of Cell Membrane Rupture under H2O2 Treatment
- Author
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Ze-Rui Zhou, Da-Wei Li, Xiao-Yuan Wang, Jian Lv, Ruo-Can Qian, and Qin Hong
- Subjects
Cell membrane ,Membrane ,medicine.anatomical_structure ,Nanosensor ,Chemistry ,Direct current ,Biophysics ,medicine ,Living cell ,Electrochemistry ,Intracellular ,Analytical Chemistry ,Label free - Abstract
H2O2 is an essential signaling molecule in living cells that can cause direct damage to lipids, proteins, and DNA, resulting in cell membrane rupture. However, current studies mostly focus on probe-based sensing of intracellular H2O2, and these methods usually require sophisticated probe synthesis and instruments. In particular, local H2O2 treatment induces cell membrane rupture, but the level of cell membrane destruction is unknown because the mechanical properties of the cell membrane are difficult to accurately determine. Therefore, highly sensitive and label-free methods are required to measure and reflect mechanical changes in the cell membrane. Here, using an ultrasmall quartz nanopipette with a tip diameter less than 90 nm as a nanosensor, label-free and noninvasive electrochemical single-cell measurement is achieved for real-time monitoring of cell membrane rupture under H2O2 treatment. By spatially controlling the nanopipette tip to precisely approach a specific location on the membrane of a single living cell, stable cyclic membrane oscillations are observed under a constant direct current voltage. Specifically, upon nanopipette advancement, the mechanical status of the cell membrane can be sensibly displayed by continuous current versus time traces. The electrical signals are collected and processed, ultimately revealing the mechanical properties of the cell membrane and the degree of cell apoptosis. This nanopipette-based nanosensor paves the way for developing a facile, label-free, and noninvasive strategy to assay the mechanical properties of the cell membrane during external stimulation at the single-cell level.
- Published
- 2021
6. In Situ Monitoring of Hydrogen Peroxide Released from Living Cells Using a ZIF-8-Based Surface-Enhanced Raman Scattering Sensor
- Author
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Cai-Hong He, Mahmoud Elsayed Hafez, Ruo-Can Qian, Cheng-Ye Xi, Essy Kouadio Fodjo, Hua-Ying Chen, Ze-Rui Zhou, Lei Jiang, and Da-Wei Li
- Subjects
In situ ,Detection limit ,Metal Nanoparticles ,Nanotechnology ,Biosensing Techniques ,Hydrogen Peroxide ,Spectrum Analysis, Raman ,Analytical Chemistry ,Catalysis ,chemistry.chemical_compound ,symbols.namesake ,chemistry ,Colloidal gold ,Zeolites ,symbols ,Molecule ,Gold ,Hydrogen peroxide ,Biosensor ,Raman scattering - Abstract
Hydrogen peroxide (H2O2) widely involves in intracellular and intercellular redox signaling pathways, playing a vital role in regulating various physiological events. Nevertheless, current analytical methods for the H2O2 assay are often hindered by relatively long response time, low sensitivity, or self-interference. Herein, a zeolitic imidazolate framework-8 (ZIF-8)-based surface-enhanced Raman scattering (SERS) sensor has been developed to detect H2O2 released from living cells by depositing ZIF-8 over SERS active gold nanoparticles (AuNPs) grafted with H2O2-responsive probe molecules, 2-mercaptohydroquinone. Combining the superior fingerprint identification of SERS and the highly efficient enrichment and selective response of H2O2 by ZIF, the ZIF-8-based SERS sensor exhibits a high anti-interference ability for H2O2 detection, with a limit of detection as low as 0.357 nM. Satisfyingly, owing to the enhanced catalytic activity derived from the successful integration of AuNPs and ZIF, the response time as short as 1 min can be obtained, demonstrating the effectiveness of the SERS sensor for rapid H2O2 detection. Furthermore, the developed SERS sensor enables real-time detection of H2O2 secreted from living cells under phorbol myristate acetate stimulation, as cells can be cultured on-chip. This study will pave the way toward the development of a metal-organic framework-based SERS platform for application in the fields of biosensing and early disease diagnosis associated with H2O2 secretion, thus exhibiting promising potential for future therapies.
- Published
- 2021
7. AuNPs-COFs Core-Shell Reversible SERS Nanosensor for Monitoring Intracellular Redox Dynamics
- Author
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Zhen-Chi Chen, Han-Bin Xu, Hua-Ying Chen, Shi-Cheng Zhu, Wen-Fei Huang, Yue He, Mahmoud Elsayed Hafez, Ruo-Can Qian, and Da-Wei Li
- Subjects
Metal Nanoparticles ,Ascorbic Acid ,Gold ,Spectrum Analysis, Raman ,Oxidation-Reduction ,Metal-Organic Frameworks ,Analytical Chemistry ,Hypochlorous Acid - Abstract
The redox homeostasis in living cells is greatly crucial for maintaining the redox biological function, whereas accurate and dynamic detection of intracellular redox states still remains challenging. Herein, a reversible surface-enhanced Raman scattering (SERS) nanosensor based on covalent organic frameworks (COFs) was prepared to dynamically monitor the redox processes in living cells. The nanosensor was fabricated by modifying the redox-responsive Raman reporter molecule, 2-Mercaptobenzoquione (2-MBQ), on the surface of gold nanoparticles (AuNPs), followed by the in situ coating of COFs shell. 2-MBQ molecules can repeatedly and quickly undergo reduction and oxidation when successively treated with ascorbic acid (AA) and hypochlorite (ClO
- Published
- 2022
8. Nanopipette-Based Nanosensor for Label-Free Electrochemical Monitoring of Cell Membrane Rupture under H
- Author
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Xiao-Yuan, Wang, Jian, Lv, Qin, Hong, Ze-Rui, Zhou, Da-Wei, Li, and Ruo-Can, Qian
- Subjects
Cell Membrane ,Hydrogen Peroxide - Abstract
H
- Published
- 2021
9. Ultrafast Mapping of Subcellular Domains via Nanopipette-Based Electroosmotically Modulated Delivery into a Single Living Cell
- Author
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Jian Lv, Ruo-Can Qian, and Yi-Tao Long
- Subjects
Capillary action ,Chemistry ,02 engineering and technology ,Living cell ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Working range ,Biophysics ,MCF-7 Cells ,Microscopy, Electron, Scanning ,Humans ,Nanotechnology ,Breast cancer cells ,Electroosmosis ,Single-Cell Analysis ,0210 nano-technology ,Ultrashort pulse ,Fluorescent Dyes ,Subcellular Fractions - Abstract
Recently, a variety of strategies have been developed for single-cell detection. However, the precise probing of the given area at single-cell level is still a challenge. Here, we put forward a rapid and targeted imaging approach for the mapping of subcelluar domains, which realizes the precise injection of multifluorescence into a single living cell via an ultrasmall quartz capillary nanopipette (∼100 nm) and can successfully transport different fluorescent probe molecules to the pointing subcellullar area around the tip in the cytoplasm within 20 s. This method is also applied for monitoring the loss of intracellular mitochondrial membrane potential under the treatment of metformin in a single MCF-7 breast cancer cell. The major driven force in the nanopipette, electroosmotic flow, is evaluated by a theory calculation method and finite element simulations, and the solution indicates a confined solute distribution profile around the tip within the working range. Overall, the nanopipette approach realizes the precise and simultaneous delivery of multiple probe molecules into the single living cell through the electroosmotically modulated, nondestructive, and one-step injection, which is especially powerful and convenient for multichannel single-cell imaging and monitoring, indicating favorable potential for understanding, monitoring, and controlling the biological processes from the single cell to subcellular organelles.
- Published
- 2018
10. Electrocatalytic Efficiency Analysis of Catechol Molecules for NADH Oxidation during Nanoparticle Collision
- Author
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Li-Jun Zhao, He Tian, Yi-Tao Long, Wei Ma, and Ruo-Can Qian
- Subjects
Catechol ,Chemistry ,Inorganic chemistry ,Nanoparticle ,Ultramicroelectrode ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,Electrocatalyst ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Catalysis ,chemistry.chemical_compound ,Colloidal gold ,Molecule ,0210 nano-technology - Abstract
Electrocatalysis of molecules is a hot research topic in biological and energy-related chemistry. Here, we develop a new system to study the electrocatalytic efficiency of a single catechol molecule for NADH oxidation by single functionalized nanoparticle collision at ultramicroelectrodes (UMEs). The proposed system is composed of gold nanoparticles (AuNPs) functionalized with catechol molecules and a carbon-fiber ultramicroelectrode. In the absence of NADH, when a functionalized AuNP collides with an UME at a suitable voltage, a small current spike is generated due to the oxidation of catechol molecules modified on the surface of AuNP. In the presence of NADH, the current spike is significantly amplified by the combined effects of the oxidation and electrocatalysis for NADH of catechol molecules. By analyzing the variations of the average peak charges and durations without or with NADH, we calculate that around five thousands NADH molecules could be catalyzed per second by a single catechol molecule, suggesting the successful establishment of this novel catalytic system. Thus, the proposed strategy could be used as a promising platform for research of other molecular electrocatalytic systems.
- Published
- 2016
11. Binary System for MicroRNA-Targeted Imaging in Single Cells and Photothermal Cancer Therapy
- Author
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Ruo-Can Qian, Yue Cao, and Yi-Tao Long
- Subjects
Infrared Rays ,Mice, Nude ,Breast Neoplasms ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Analytical Chemistry ,Metastasis ,Mice ,Single-cell analysis ,Molecular beacon ,microRNA ,medicine ,Tumor Cells, Cultured ,Animals ,Humans ,Particle Size ,Mice, Inbred BALB C ,Chemistry ,Temperature ,Mammary Neoplasms, Experimental ,Photothermal therapy ,Phototherapy ,021001 nanoscience & nanotechnology ,medicine.disease ,Photochemical Processes ,Molecular biology ,0104 chemical sciences ,MicroRNAs ,Cytoplasm ,Colloidal gold ,Cancer cell ,Biophysics ,MCF-7 Cells ,Female ,Single-Cell Analysis ,0210 nano-technology - Abstract
Abnormal expression of microRNAs (miRNAs) is often associated with tumorigenesis, metastasis, and progression. Among them, miRNA-21 is found to be overexpressed in most of the cancer cells. Here, a binary system is designed for miRNA-21 targeted imaging and photothermal treatment in single cells. The binary system is composed by a pair of probes (probe-1 and probe-2), which are encapsulated in liposomes for cell delivery. Both of the two probes adopt gold nanoparticles (AuNPs) as the core material, and the AuNPs are functionalized with Cy5-marked molecular beacon (MB-1/MB-2 for probe-1/probe-2, respectively). The loop part of MBs are designed to be complementary with miRNA-21. Therefore, after the binary system enters into the cytoplasm, MBs can be opened upon miRNA-21 triggered hybridization, which turns "on" the fluorescence of Cy5 for the localization of miRNA-21. At the same time, a cross-linking between the probes occurs since the far ends of MB-1 and MB-2 are designed to be complementary with each other. The miRNA-induced aggregation shifts the absorption of AuNPs to near-infrared, which can be observed under dark-field microscopy (DFM) and used for the following photothermal therapy. Under near-infrared (NIR) irradiation, MCF-7 breast cancer cells are successfully killed. The proposed system can be further applied in tumor-bearing mice and shows significant therapeutic effect. This work provides a new tool for intracellular miRNA analysis and targeted treatment against cancer.
- Published
- 2016
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