1. Near-Infrared Probe Based on Rhodamine Derivative for Highly Sensitive and Selective Lysosomal pH Tracking.
- Author
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Niu, Guangle, Zhang, Panpan, Liu, Weimin, Wang, Mengqi, Zhang, Hongyan, Wu, Jiasheng, Zhang, Liping, and Wang, Pengfei
- Subjects
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FLUORESCENT probes , *RHODAMINES , *PH effect , *LYSOSOMES , *CHEMICAL derivatives , *NEAR infrared radiation - Abstract
The development of near-infrared fluorescent probes with low pKa, high selectivity, high photostability, and high sensitivity for lysosomal pH detection is of great importance. In the present work, we developed a novel near-infrared lysosomal pH probe (Lyso-hNR) based on a rhodamine derivative. Lyso-hNR showed fast, highly sensitive, and highly selective fluorescence response to acidic pH caused by the H+-induced structure changes from the nonfluorescent spirolactam form to the highly emissive open-ring form. Lyso-hNR displays a significant fluorescence enhancement at 650 nm (over 280-fold) from pH 7.0 to 4.0 with a pKa value of 5.04. Live cell imaging data revealed that Lyso-hNR can selectively monitor lysosomal pH changes with excellent photostability and low cytotoxicity. In addition, Lyso-hNR can be successfully used in tracking lysosomal pH changes induced by chloroquine and those during apoptosis. All these features render Lyso-hNR a promising candidate to investigate lysosome-associated physiological and pathological processes. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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