1. Chemical-Modified Nucleotide-Based Elemental Tags for High-Sensitive Immunoassay
- Author
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Fujian Xu, Xinrong Zhang, Zhi Xing, Sichun Zhang, Zhian Hu, Gongwei Sun, Yuqing Zhang, Xingyu Pan, Meng-Chan Xia, and Wencan Jiang
- Subjects
Immunoassay ,medicine.diagnostic_test ,Biocompatibility ,Chemistry ,010401 analytical chemistry ,Oligonucleotides ,010402 general chemistry ,01 natural sciences ,Fluorescence ,Combinatorial chemistry ,Orders of magnitude (mass) ,Mass Spectrometry ,0104 chemical sciences ,Analytical Chemistry ,Alkynes ,medicine ,Click chemistry ,Humans ,Multiplex ,Mass cytometry ,Metals, Rare Earth ,alpha-Fetoproteins ,Inductively coupled plasma mass spectrometry - Abstract
Multiplex biomolecular analysis with inductively coupled plasma mass spectrometry (ICP-MS) becomes increasingly important in clinical diagnosis and single cell analysis. However, the sensitivity of ICP-MS-based immunoassay is only comparable or lower than those of fluorescence methods at the present stage. Therefore, designing elemental tags with a large number of metal atoms is necessary to achieve high-sensitive detection. In this work, we proposed a new strategy to build up elemental tag loading with hundreds of rare earth ions by coupling alkyne-DNA chains with rare earth element (REE)-DOTA complexes a click chemistry reaction. There are about 2 orders of magnitude improvement in sensitivity compared with single metal-ion tags. DNA chains with multialkynyl groups were facilely prepared by PCR synthesis. Moreover, the DNA-based elemental tags own excellent water-solubility and biocompatibility. The tags would be potentially applied to mass cytometry and clinical diagnosis.
- Published
- 2019