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1. Synthesis and in situ insertion of a site-specific fluorescently labeled membrane protein into cell-sized liposomes.

Author

Ohtsuka T, Neki S, Kanai T, Akiyoshi K, Nomura SM, and Ohtsuki T

Subjects
Amino Acids chemistry, Bacteriorhodopsins genetics, Codon, Fluorescent Dyes chemistry, Microscopy, Confocal, Mutagenesis, Insertional, RNA, Messenger genetics, RNA, Transfer genetics, Bacteriorhodopsins chemistry, Liposomes chemistry, Spectrometry, Fluorescence methods
Abstract

The integral membrane protein bacteriorhodopsin, containing a fluorescent amino acid at a specific position, was synthesized in the presence of hydrated lipid films using an in vitro translation system expanded with a four-base codon/anticodon pair. Cell-sized liposomes with the labeled protein inserted into the liposome membranes were generated after the translation reaction. This study also demonstrated that this labeling method could be used to analyze the dynamic properties of membrane proteins in situ by fluorescence correlation spectroscopy., (Copyright © 2011 Elsevier Inc. All rights reserved.)

Published
2011
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