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Start Over Author "Korytnyk W" Journal analytical biochemistry
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5. A high-performance liquid chromatography method for the assay of cytidine monophosphate-sialic acid synthetase.

Author

Petrie CR 3rd and Korytnyk W

Subjects
Animals, Cattle, Chromatography, High Pressure Liquid, Colorimetry, Cytidine Monophosphate metabolism, Kinetics, Sialic Acids metabolism, N-Acylneuraminate Cytidylyltransferase analysis, Nucleotidyltransferases analysis
Abstract

An HPLC method has been developed for the assay of cytidine monophosphate-sialic acid synthetase (EC 2.7.7.43) using ion-pair chromatography and gradient elution. This procedure permits the assay of alternative substrates and inhibitors of the enzyme and is not subject to the limitation of the colorimetric method. The newly synthesized N-acetyl-9-deoxy-9-fluoro-D-neuraminic acid was found to be a good substrate of the enzyme with a Km of 6.35 mM as compared to 1.84 mM for N-acetylneuraminic acid.

Published
1983
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