1. Alkylation of acidic nuclear proteins: a means of simplifying analyses and improving fractionation
- Author
-
Edward Bresnick and Jim Blankenship
- Subjects
Male ,Reducing agent ,Biophysics ,Iodoacetates ,Fractionation ,Plasma protein binding ,Alkylation ,Biochemistry ,chemistry.chemical_compound ,Column chromatography ,Acetamides ,Methods ,Animals ,Nuclear protein ,Molecular Biology ,Gel electrophoresis ,Cell Nucleus ,Chromatography ,Binding Sites ,Chemistry ,Cell Biology ,Hydrogen-Ion Concentration ,Rats ,Nucleoproteins ,Liver ,Evaluation Studies as Topic ,Iodoacetamide ,Electrophoresis, Polyacrylamide Gel ,Ultracentrifugation ,Densitometry ,Protein Binding - Abstract
Reducing agents are commonly used to limit aggregation of acidic nuclear proteins in aqueous media. However, these agents interfere with protein determinations and thus make it difficult to continuously monitor protein concentration. In this study, acidic nuclear proteins were alkylated with iodoacetamide to eliminate the need for a reducing agent. The alkylated proteins can be easily analyzed; and furthermore, alkylation may enhance the resolution obtained by column chromatography or gel electrophoresis.
- Published
- 1974