1. Detection of carcinoembryonic antigen using single-domain or full-size antibodies stained with quantum dot conjugates
- Author
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Brigitte Reveil, Klervi Even-Desrumeaux, Jacques H. M. Cohen, Daniel Baty, Igor Nabiev, Kristina Brazhnik, Alyona Sukhanova, Thierry Tabary, Gilles Rousserie, Regina Grinevich, Patrick Chames, Laboratoire de Recherche en Nanosciences - EA 4682 (LRN), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), and Research and Development in Priority Areas of Development of the Russian Scientific and Technological Complex (14.575.21.0065)
- Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,MESH: Flow Cytometry ,MESH: Amino Acid Sequence ,Biochemistry ,MESH: Antibodies, Monoclonal ,chemistry.chemical_compound ,Mice ,Carcinoembryonic antigen ,MESH: Animals ,biology ,medicine.diagnostic_test ,Antibodies, Monoclonal ,MESH: Quantum Dots ,Flow Cytometry ,Fluorescence ,3. Good health ,Biotinylation ,Streptavidin ,MESH: Carcinoembryonic Antigen ,medicine.drug_class ,Molecular Sequence Data ,Biophysics ,Monoclonal antibody ,MESH: Single-Domain Antibodies ,Flow cytometry ,Cell Line ,In vivo ,Carcinoembryonic antigen (CEA) ,Quantum Dots ,medicine ,Animals ,Humans ,MESH: Biotinylation ,Amino Acid Sequence ,Molecular Biology ,MESH: Mice ,MESH: Humans ,MESH: Molecular Sequence Data ,technology, industry, and agriculture ,Cell Biology ,Single-Domain Antibodies ,equipment and supplies ,Molecular biology ,In vitro ,digestive system diseases ,MESH: Cell Line ,Carcinoembryonic Antigen ,chemistry ,biology.protein ,Single-domain antibodies (sdAbs) - Abstract
International audience; Compact single-domain antibodies (sdAbs) are nearly 13 times smaller than full-size monoclonal antibodies (mAbs) and have a number of advantages for biotechnological applications, such as small size, high specificity, solubility, stability, and great refolding capacity. Carcinoembryonic antigen (CEA) is a tumor-associated glycoprotein expressed in a variety of cancers. Detection of CEA on the tumor cell surface may be carried out using anti-CEA antibodies and conventional fluorescent dyes. Semiconductor quantum dots (QDs) are brighter and more photostable than organic dyes; they provide the possibility for labeling of different recognition molecules with QDs of different colors but excitable with the same wavelength of excitation. In this study, the abilities for specific detection of CEA expressed by tumor cells with anti-CEA sdAbs biotinylated in vitro and in vivo, as well as with anti-CEA mAbs biotinylated in vitro, were compared using flow cytometry and the conjugates of streptavidin with QDs (SA-QDs). The results demonstrated that either in vitro or in vivo biotinylated anti-CEA sdAbs are more sensitive for cell staining compared to biotinylated anti-CEA mAbs. The data also show that simultaneous use of biotinylated sdAbs with highly fluorescent SA-QDs can considerably improve the sensitivity of detection of CEA on tumor cell surfaces.
- Published
- 2014