1. Differential density separation of cellular suspensions
- Author
-
Robert Ballentine and Dorothy D. Burford
- Subjects
Chromatography ,Suspensions ,Chemistry ,Density separation ,Biophysics ,Humans ,Centrifugation ,Cell Biology ,Contamination ,Molecular Biology ,Biochemistry ,Chemistry Techniques, Analytical ,Suspension (chemistry) - Abstract
A method is described for the rapid separation of cells from their suspending medium with only 1 5 th to 1 30 th the amount of contamination from the medium as with conventional centrifuging. The principle is to interlayer between the cells and the medium upon centrifuging an immiscible liquid whose density lies between that of the cells and medium. Quantitative measurement of the amount of contamination of the cells by the medium which does occur is achieved by adding a nonpermeable radioactively labeled substance to the suspension prior to centrifugation.
- Published
- 1960
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