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Your search keyword '"C Van den Branden"' showing total 2 results
Start Over Author "C Van den Branden" Journal analytical and quantitative cytology and histology
2 results on '"C Van den Branden"'

1. Automated measurements on cell nuclei in tissue sections. Validation procedures

Author

A, Claeys, C, Van den Branden, and F, Roels

Subjects
Cell Nucleus, Microscopy, Staining and Labeling, Adenocarcinoma, Rats, Pancreatic Neoplasms, Adrenocorticotropic Hormone, Adrenal Cortex, Image Processing, Computer-Assisted, Rosaniline Dyes, Animals, Humans, Zona Fasciculata, Coloring Agents
Abstract

A methodology was developed for fully automated measurements of nuclear features in Feulgen-stained tissue sections by means of videomicroscopy and image analysis. Segmentation is performed within one minute on 512 X 512 optical density (OD) images covering about 75 nuclei, resulting in a graphic contour overlay. The corresponding image subset is scanned by an object data extraction program, producing the raw figures for statistical interpretation. The segmentation software was evaluated by three tests, involving comparison with manual delineation and assessment of the influence of OD. Two case studies (ACTH-stimulated adrenal cortex and pancreatic carcinoma) illustrate the biologic accuracy and medical significance of the described methodology.

Published
1988

2. Automated measurements on cell nuclei in tissue sections. Validation procedures.

Author

Claeys A, Van den Branden C, and Roels F

Subjects
Adenocarcinoma ultrastructure, Adrenal Cortex drug effects, Adrenal Cortex ultrastructure, Adrenocorticotropic Hormone pharmacology, Animals, Cell Nucleus drug effects, Coloring Agents, Humans, Microscopy, Pancreatic Neoplasms ultrastructure, Rats, Staining and Labeling, Zona Fasciculata ultrastructure, Cell Nucleus ultrastructure, Image Processing, Computer-Assisted methods, Rosaniline Dyes
Abstract

A methodology was developed for fully automated measurements of nuclear features in Feulgen-stained tissue sections by means of videomicroscopy and image analysis. Segmentation is performed within one minute on 512 X 512 optical density (OD) images covering about 75 nuclei, resulting in a graphic contour overlay. The corresponding image subset is scanned by an object data extraction program, producing the raw figures for statistical interpretation. The segmentation software was evaluated by three tests, involving comparison with manual delineation and assessment of the influence of OD. Two case studies (ACTH-stimulated adrenal cortex and pancreatic carcinoma) illustrate the biologic accuracy and medical significance of the described methodology.

Published
1988

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