1. Combined fiber probe for fluorescence lifetime and Raman spectroscopy
- Author
-
Thomas Bocklitz, Hussain Fatakdawala, Brad A. Hartl, Jürgen Popp, Julien Bec, Michael Schmitt, Ines Latka, Eric Marple, Sebastian Dochow, Dinglong Ma, Sebastian Wachsmann-Hogiu, Laura Marcu, and Kirk Urmey
- Subjects
inorganic chemicals ,Fluorescence-lifetime imaging microscopy ,Optical fiber ,Materials science ,Bioengineering ,macromolecular substances ,Spectrum Analysis, Raman ,Biochemistry ,Article ,Imaging ,Quantitative Biology::Cell Behavior ,Analytical Chemistry ,law.invention ,symbols.namesake ,Engineering ,Nuclear magnetic resonance ,Fluorescence lifetime ,law ,Fiber probes ,Animals ,Humans ,Fiber Optic Technology ,Raman ,Brain Chemistry ,Inbred F344 ,Spectrum Analysis ,Optical Imaging ,technology, industry, and agriculture ,Endoscopy ,Equipment Design ,Biological Sciences ,Fluorescence ,Rats, Inbred F344 ,Rats ,Bimodal ,Raman spectroscopy ,Chemical Sciences ,symbols ,Dual modality ,Fiber probe ,Excitation ,Visible spectrum - Abstract
In this contribution we present a dual modality fiber optic probe combining fluorescence lifetime imaging (FLIm) and Raman spectroscopy for in vivo endoscopic applications. The presented multi-spectroscopy probe enables efficient excitation and collection of fluorescence lifetime signals for FLIm in the UV/visible wavelength region, as well as of Raman spectra in the near-IR for simultaneous Raman/FLIm imaging. The probe was characterized in terms of its lateral resolution and distance dependency of the Raman and FLIm signals. In addition, the feasibility of the probe for in vivo FLIm and Raman spectral characterization of tissue was demonstrated. Graphical Abstract An image comparison between FLIm and Raman spectroscopy acquired with the bimodal probe onseveral tissue samples.
- Published
- 2015