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Determining organic micropollutants in liver samples of apex species is of foremost importance for biomonitoring studies, as it can provide evidence of environmental pollution and exposure of living organisms to chemicals. This review aims to provide a 4-year overview and summarize the trends in the extraction methodologies to determine both polar and non-polar organic micropollutants in liver samples from organisms of higher trophic levels. The dominant extraction techniques including ultrasound-assisted extraction (UAE), pressurized liquid extraction (PLE), Soxhlet, and QuEChERS, as well as additional steps and/or modifications applied in the reviewed studies, are presented and critically discussed. The latest trends in these methods as well as a comparison between them considering elapsed time, robustness, cost, and environmental fingerprint are also provided., Competing Interests: Declarations. Conflict of interest: The authors declare that they have no known financial conflicts of interest or personal relationships that may have influenced the research presented in this paper. Nikolaos S. Thomaidis is a guest editor of ABC but was not involved in the peer review of this paper., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)