1. Fast chromatographic determination of the bile salt critical micellar concentration.
- Author
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Natalini, Benedetto, Sardella, Roccaldo, Gioiello, Antimo, Rosatelli, Emiliano, Ianni, Federica, Camaioni, Emidio, and Pellicciari, Roberto
- Subjects
BILE acids ,CHROMATOGRAPHIC analysis ,CRITICAL micelle concentration ,MOLECULES ,MATHEMATICAL models - Abstract
In a line of research focused on the design, synthesis and development of new bile acid-based compounds, the physico-chemical profile of the molecules must be thoroughly explored and analyzed. In this scenario, a fast and reliable information on the critical micellar concentration (CMC) of specific compounds through a profitable chromatographic parameter can be of aid to rationally direct the synthesis of new molecular entities, mainly during the early stages of the drug-discovery process. The derived 'chromatographic hydrophobicity index' (CHI), usually employed for a fast access to the log P/log D value of physico-chemically diverse compounds and obtained via RP-gradient elution, was for the first time engaged in the bile acid field. Accordingly, 14 unconjugated bile acids harboured with a different number, position and orientation of hydroxy groups, as well as other substituents onto the steroidal backbone and side chain, were selected to build up a calibration curve. Such a collection of compounds was rationally assembled in order to manage an almost continuous range of CMC values (spanning the spectrophotometrically obtained CMCs between 5 and 25 mM). A high degree of correlation between CMC and CHI values was obtained ( R and cross-validated R of the pCMC vs CHI plot equal to 0.975 and 0.966, respectively). A selected new subset of five confidential research bile acids with experimental CMCs in the range 6-19 mM was finally recruited to validate the proposed method. The high statistical quality of the established mathematical model turned out into a very appreciable predictive power. [Figure not available: see fulltext.] [ABSTRACT FROM AUTHOR]
- Published
- 2011
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