1. Simultaneous LC-MS/MS determination of JWH-210, RCS-4, ∆-tetrahydrocannabinol, and their main metabolites in pig and human serum, whole blood, and urine for comparing pharmacokinetic data.
- Author
-
Schaefer, Nadine, Kettner, Mattias, Laschke, Matthias, Schlote, Julia, Peters, Benjamin, Bregel, Dietmar, Menger, Michael, Maurer, Hans, Ewald, Andreas, and Schmidt, Peter
- Subjects
- *
TETRAHYDROCANNABINOL , *METABOLITE analysis , *LIQUID chromatography-mass spectrometry , *BLOOD serum analysis , *URINALYSIS , *BLOOD testing , *PHARMACOKINETICS , *SWINE physiology - Abstract
A series of new synthetic cannabinoids (SC) has been consumed without any toxicological testing. For example, pharmacokinetic data have to be collected from forensic toxicological case work and/or animal studies. To develop a corresponding model for assessing such data, samples of controlled pig studies with two selected SC (JWH-210, RCS-4) and, as reference, ∆-tetrahydrocannabinol (THC) should be analyzed as well as those of human cases. Therefore, a method for determination of JWH-210, RCS-4, THC, and their main metabolites in pig and human serum, whole blood, and urine samples is presented. Specimens were analyzed by liquid-chromatography tandem mass spectrometry and multiple-reaction monitoring with three transitions per compound. Full validation was carried out for the pig specimens and cross-validation for the human specimens concerning precision and bias. For the pig studies, the limits of detection were between 0.05 and 0.50 ng/mL in serum and whole blood and between 0.05 and 1.0 ng/mL in urine, the lower limits of quantification between 0.25 and 1.0 ng/mL in serum and 0.50 and 2.0 ng/mL in whole blood and urine, and the intra- and interday precision values lower than 15 % and bias values within ±15 %. The applicability was tested with samples taken from a pharmacokinetic pilot study with pigs following intravenous administration of a mixture of 200 μg/kg body mass dose each of JWH-210, RCS-4, and THC. The cross-validation data for human serum, whole blood, and urine showed that this approach should also be suitable for human specimens, e.g., of clinical or forensic cases. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF