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Herein, a three-dimensional graphene wall (GWs) and nano-Cu 2 O modified carbon fiber paper (CFP) electrode were used to develop a disposable and sensitive non-enzymatic glucose sensor. This sensing interface of GWs/Cu 2 O consists of an interlaced CFP on which intercrossed graphene walls (GWs) were vertically tethered in situ by radio frequency plasma enhanced chemical vapor deposition (RF-PECVD), and Cu 2 O nanoparticles (NPs) were evenly grew on the 3D GWs layer and skeleton through the complete thermal decomposition of copper acetate (Cu (CH 3 COO) 2 ) at high temperature. The CFP/GWs/Cu 2 O shows a large specific surface area and rich solution diffusion channels, which can expose more catalytic active sites without Nafion fixation film, thus greatly improving the electrocatalytic performance of this glucose sensor. The CFP/GWs/Cu 2 O sensor shows excellent catalytic performance to glucose with a linear detection range of 0.5 μM-5166 μM, LOD of 0.21 μM, and response time <4 s. This kind of disposable and sensitive electrode can capable of controlling uniform growth and accurate quantification, and has great development potential in the field of medical detection and the commercialization of wearable sensors., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)