1. Selenium metabolomics in yeast using complementary reversed-phase/hydrophilic ion interaction (HILIC) liquid chromatography–electrospray hybrid quadrupole trap/Orbitrap mass spectrometry
- Author
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C. Arnaudguilhem, Laurent Ouerdane, Katarzyna Bierla, Ryszard Lobinski, Hugues Preud'homme, Alexandros Yiannikouris, Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), and Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Spectrometry, Mass, Electrospray Ionization ,Electrospray ,Speciation ,Electrospray ionization ,Analytical chemistry ,Saccharomyces cerevisiae ,Mass spectrometry ,Orbitrap ,01 natural sciences ,Biochemistry ,High-performance liquid chromatography ,Analytical Chemistry ,law.invention ,Selenium ,03 medical and health sciences ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,law ,Metabolomics ,Environmental Chemistry ,Inductively coupled plasma mass spectrometry ,Chromatography, High Pressure Liquid ,Spectroscopy ,030304 developmental biology ,Detection limit ,Chromatography, Reverse-Phase ,0303 health sciences ,Chromatography ,Chemistry ,Hydrophilic interaction chromatography ,010401 analytical chemistry ,ICP MS ,Porous graphitic carbon ,0104 chemical sciences ,Hydrophobic and Hydrophilic Interactions - Abstract
International audience; A high efficiency chromatographic separation on a porous graphitic carbon stationary phase was developed for a large-scale separation of selenium metabolites in Se-rich yeast prior to their identification by electrospray hybrid quadrupole trap/Orbitrap mass spectrometry (Orbitrap MSn). The reversed-phase (RP) separation mode offered distinctly higher separation efficiency than the hydrophilic ion interaction (HILIC) mode. The latter was nevertheless complementary and useful to validate the detection of several compounds. The method allowed the detection of 64 metabolites including 30 SeSe or SeS conjugates (3 triple S/Se/S ones) and 14 selenoethers. 21 previously unreported metabolites were detected on the basis of the selenium isotopic pattern usually matched with the sub-ppm mass accuracy. 9 of these metabolites were subsequently identified using the multi-stage high mass accuracy (
- Published
- 2012