1. Accurate phosphorylation site localization using phospho-brackets.
- Author
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Xiao, Kaijie, Shen, Yun, Li, Shasha, and Tian, Zhixin
- Subjects
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PHOSPHORYLATION , *POST-translational modification , *PHOSPHOPEPTIDES , *PROTEOMICS , *TANDEM mass spectrometry - Abstract
Phosphorylation is one of the most important and widely studied protein post-translational modifications. Tandem mass spectrometry using higher-energy collisional dissociation has evolved into a state-of-the-art analytical platform for both phosphorylation identification and site localization. Tens of thousands of phosphopeptides can now be routinely identified from a single shotgun proteomics study; site localization, however, is much more complicated and many challenges still exist. Here, we report our development of P-bracket using direct experimental evidence of phospho-containing site-determining product ions for accurate site localization without the need for additional FLR control. A P-bracket is defined as a complementary product ion pair that forms a bracket to confine a phosphorylation event to a unique site. P-bracket has been successfully benchmarked with a set of six synthetic phosphopeptides with a single phosphorylation event, a set of 96 synthetic peptides and phosphopeptide reference libraries, and two HeLa phosphopeptide LC-MS/MS (HCD) datasets; Accurate phosphosite localization by P-bracket will greatly enhance identification confidence of phosphopeptides and contribute to structural and functional studies of phosphoproteins. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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