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A smart plasmonic sensor, comprising a layer of a stimuli-responsive polymer sandwiched between two gold layers, is reported. As a stimuli-responsive material, a monolayer of poly(N-isopropylacrylamide) (PNIPAm) crosslinked globules is used. A quasi-periodic structure of the top gold layer facilitates efficient excitation and serves as a support for plasmon excitation and propagation. The intermediate layer of PNIPAm efficiently entraps targeted molecules from solutions. The sensor structure was optimized for efficient light focusing in the “active” PNIPAm layer. The optimization was based on the time-resolved finite-element simulations, which take into account the thickness of gold layers, size of PNIPAm globules and Raman excitation wavelength (780 nm). The prepared structures were characterized using SEM, AFM, UV-Vis refractometry and goniometry. Additional AFM scans were performed in water at two temperatures corresponding to the collapsed and swollen PNIPAm states. The Raman measurements demonstrate a high detection limit and perfect reproducibility of the Raman scattering signal for the prepared sensor. In addition, the use of created SERS structures for the detection of relevant molecules in the medical, biological and safety fields was demonstrated. [ABSTRACT FROM AUTHOR]

Detection of trace amounts of poorly water-soluble pharmaceuticals or related (bio)solutions represents a key challenge in environment protection and clinical diagnostics. However, this task is complicated by low concentrations of pharmaceuticals, complex sample matrices, and sophisticated sample preparative routes. In this work, we present an alternative approach on the basis of an on-line flow extraction procedure and SERS measurements performed in a microfluidic regime. The advantages of our approach were demonstrated using ibuprofen (Ibu), which is considered as a common pharmaceutical contaminant in wastewater and should be monitored in various bioliquids. The extraction of Ibu from water to the dichloromethane phase was performed with an optimized microfluidic mixer architecture. As SERS tags, lipophilic functionalized gold multibranched nanoparticles (AuMs) were added to the organic phase. After microfluidic extraction, Ibu was captured by the functionalized AuM surface and recognized by on-line SERS measurements with up to 10-8 M detection limit. The main advantages of the proposed approach can be regarded as its simplicity, lack of need for preliminary sample preparation, high reliability, the absence of sample pretreatment, and low detection limits.