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The aim of this study was to establish the PAG dynamics at sheep and goat in the pregnancy first stage (1-40 days after mating) and its correlation with the females physiological status in the mean to predict pregnancy certainly. The pregnancy-associated glycoproteins were determinate by radioimmunoassay established by Beckers -Zoli. The blood samples were collected from 18 goats, in day 0 (mating day), 10, 25 and 40. The separated plasma was preserved at -18°C until the RIA application. The RIA was performed in FMV-Liege Belgium- Reproduction Physiology Laboratory using the preincubation method an radio marked (I125 ) antigen bPAG. The samples were duplicated analyzed and we used the standard curve to establish the PAG's values (ng/ml). The radioactivity was readed at Wallac-1261 Multigamma Counter which is equipped with specific software and PAG concentration exhibition. The obtained PAG values were: in day 0 de 0,1- 1,5 ng/ml , in day 10 10-14 ng/ml , in 25 day from mating 20-39,7 ng /ml , and 40-47,9 ng/ml in day 40. In not pregnant females the PAG values were higher than 10-20 ng /ml. After female term parturition it was settled that 12 females were surely pregnant and 6 was not at all. Analyzing the pregnancy probability using PAG values in days 0, 10, 25 and 40, the predictability index for pregnancy was 91,66% and for not pregnancy 66,66%. [ABSTRACT FROM AUTHOR]

The transversal deformation response force is an useful parameter for in vivo evaluation of skeletal muscle contractile response in basal conditions, electrical stimulation (induced tetany) or after myorelaxing drugs administration. The present device allows the recording of the transversal deformation response force which represents the parameter for the evaluation of skeletal muscle tone. The purpose of this study was to evaluate the in vivo relaxing effects of a new purified Helleborus Purpurascens root extract (ACSw05) on skeletal muscle tone, using an experimental device. In this study we used Sprague Dawley rats (200-250g), that were immobilized on the contention table, after they have been anesthetized with Nembutal 20 mg/kg i.p. The evaluation of ACSw05 extract effects on skeletal muscle tone was performed by analyzing the plotted length-force curves; the follow-up period was 90 minutes. First we performed a control recording curve and afterwards we injected intramuscular 200 μl ACSw05 extract. We noticed a decrease of length-force curve slope and a 40% decrease of the contractile muscle response compared with the control recording curve. The relaxation curve slope hasn't shown significant changes compared to the control recording curve. The device and method for skeletal muscle tone evaluation by measuring the transversal deformation response force can be used to compare the effects of different physical, chemical factors and drugs on skeletal muscle. [ABSTRACT FROM AUTHOR]