Search

The aim of this study was to establish the PAG dynamics at sheep and goat in the pregnancy first stage (1-40 days after mating) and its correlation with the females physiological status in the mean to predict pregnancy certainly. The pregnancy-associated glycoproteins were determinate by radioimmunoassay established by Beckers -Zoli. The blood samples were collected from 18 goats, in day 0 (mating day), 10, 25 and 40. The separated plasma was preserved at -18°C until the RIA application. The RIA was performed in FMV-Liege Belgium- Reproduction Physiology Laboratory using the preincubation method an radio marked (I125 ) antigen bPAG. The samples were duplicated analyzed and we used the standard curve to establish the PAG's values (ng/ml). The radioactivity was readed at Wallac-1261 Multigamma Counter which is equipped with specific software and PAG concentration exhibition. The obtained PAG values were: in day 0 de 0,1- 1,5 ng/ml , in day 10 10-14 ng/ml , in 25 day from mating 20-39,7 ng /ml , and 40-47,9 ng/ml in day 40. In not pregnant females the PAG values were higher than 10-20 ng /ml. After female term parturition it was settled that 12 females were surely pregnant and 6 was not at all. Analyzing the pregnancy probability using PAG values in days 0, 10, 25 and 40, the predictability index for pregnancy was 91,66% and for not pregnancy 66,66%. [ABSTRACT FROM AUTHOR]