1. Effects of supplementation with branched-chain amino acids to low-protein diets on expression of genes related to lipid metabolism in skeletal muscle of growing pigs
- Author
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Yujiao Ji, Yehui Duan, Tiejun Li, Yangmiao Duan, Bie Tan, Yulong Yin, Qiuping Guo, Yinghui Li, and Fengna Li
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Low protein ,Swine ,medicine.medical_treatment ,Clinical Biochemistry ,Protein metabolism ,Muscle Proteins ,Biology ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Low-protein diet ,Valine ,Internal medicine ,medicine ,Animals ,Muscle, Skeletal ,chemistry.chemical_classification ,Organic Chemistry ,0402 animal and dairy science ,Fatty acid ,Lipid metabolism ,04 agricultural and veterinary sciences ,Lipid Metabolism ,040201 dairy & animal science ,030104 developmental biology ,Endocrinology ,Gene Expression Regulation ,chemistry ,Dietary Proteins ,Intramuscular fat ,Amino Acids, Branched-Chain ,Polyunsaturated fatty acid - Abstract
Branched-chain amino acids (BCAA), including leucine (Leu), isoleucine (Ile), and valine (Val), play critical roles in energy homeostasis and lipid metabolism in addition to their other functions, such as in protein metabolism. This study investigated the effects of different dietary BCAA ratios on the intramuscular fat (IMF) content and fatty acid composition in different location of skeletal muscles, including the longissimus dorsi (LD), biceps femoris (BF), and psoas major (PM) muscles of growing pigs, and also examined the mRNA expression levels of genes involved in lipid metabolism in these muscle tissues. The experiment was performed on 40 growing pigs (Large White × Landrace) with a similar initial weight (9.85 ± 0.35 kg). The pigs were randomly assigned to one of five diets: diet A was a positive control and contained 20 % crude protein (CP) with a Leu:Ile:Val ratio of 1:0.51:0.63 according to the recommendation of the National Research Council (NRC); for diets B to E, the CP level was reduced to 17 %, and the Leu:Ile:Val ratios were 1:1:1, 1:0.75:0.75, 1:0.51:0.63, and 1:0.25:0.25, respectively. No significant difference was observed in the average feed intake and feed efficiency of the pigs fed the low protein diet (17 % CP) with BCAA treatments relative to the positive control. However, there was a tendency for increased feed efficiency of the 1:0.75:0.75 group compared with the 1:1:1 group (P = 0.09). The BCAA ratio of 1:0.75:0.75 (17 % CP) increased the IMF content of BF muscle (P
- Published
- 2016