1. Effects of Gamma Irradiation of Human Serum Samples from rVSVΔG-ZEBOV-GP (V920) Ebola Virus Vaccine Recipients on Plaque-Reduction Neutralization Assays.
- Author
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Grant-Klein RJ, Antonello J, Nichols R, Dubey S, and Simon JK
- Subjects
- Antibodies, Neutralizing analysis, Ebola Vaccines chemical synthesis, Ebolavirus pathogenicity, Healthy Volunteers, Hemorrhagic Fever, Ebola blood, Hemorrhagic Fever, Ebola immunology, Hemorrhagic Fever, Ebola virology, Humans, Immune Sera chemistry, Immunogenicity, Vaccine, Neutralization Tests, Prospective Studies, Vaccination methods, Vesiculovirus chemistry, Vesiculovirus immunology, Viral Envelope Proteins immunology, Antibodies, Neutralizing blood, Antibodies, Viral blood, Ebola Vaccines administration & dosage, Ebolavirus immunology, Hemorrhagic Fever, Ebola prevention & control, Immune Sera radiation effects
- Abstract
Gamma irradiation (GI) is included in the CDC guidance on inactivation procedures to render a group of select agents and toxins nonviable. The Ebola virus falls within this group because it potentially poses a severe threat to public health and safety. To evaluate the impact of GI at a target dose of 50 kGy on neutralizing antibody titers induced by the rVSVΔG-ZEBOV-GP vaccine (V920), we constructed a panel of 48 paired human serum samples (GI-treated versus non-GI-treated) from healthy participants selected from a phase 3 study of V920 (study V920-012; NCT02503202). Neutralizing antibody titers were determined using a validated plaque-reduction neutralization test. GI of sera from V920 recipients was associated with approximately 20% reduction in postvaccination neutralizing antibody titers. GI was not associated with any change in pre-vaccination neutralizing antibody titers.
- Published
- 2021
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