1. Overexpression of GATA-3 Protects against the Development of Hypersensitivity Pneumonitis
- Author
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Keigyou Yoh, Satoru Takahashi, Shinsuke Homma, Tohru Sakamoto, Norihiro Kikuchi, Yosuke Matsuno, Akihiro Nomura, Yuko Morishima, Takumi Kiwamoto, Morio Ohtsuka, Nobuyuki Hizawa, Takashi Iizuka, Norihiro Haraguchi, and Yukio Ishii
- Subjects
Pulmonary and Respiratory Medicine ,medicine.medical_treatment ,Mice, Transgenic ,Inflammation ,GATA3 Transcription Factor ,Critical Care and Intensive Care Medicine ,Pathogenesis ,Interferon-gamma ,Mice ,Antigen ,Interferon ,Intensive care ,Animals ,Medicine ,Saccharopolyspora rectivirgula ,RNA, Messenger ,biology ,Tumor Necrosis Factor-alpha ,business.industry ,Interleukins ,medicine.disease ,biology.organism_classification ,Mice, Inbred C57BL ,Disease Models, Animal ,Cytokine ,Immunology ,medicine.symptom ,T-Box Domain Proteins ,business ,Hypersensitivity pneumonitis ,Alveolitis, Extrinsic Allergic ,Saccharopolyspora ,medicine.drug - Abstract
Hypersensitivity pneumonitis (HP) is mediated by a Th1 immune response. Transcription factor GATA binding protein-3 (GATA-3) is believed to be a key regulator of Th2 differentiation and thus might play regulatory roles in the pathogenesis of hypersensitivity pneumonitis (HP).We examined the effect of GATA-3 overexpression on the development of HP in mice.Wild-type C57BL/6 mice and GATA-3-overexpressing mice of the same background were used in this study. HP was induced by repeated exposure to Saccharopolyspora rectivirgula, the causative antigen of farmer's lung.Antigen exposure resulted in a marked inflammatory response with enhanced pulmonary expression of T-bet and the Th1 cytokine interferon (IFN)-gamma in wild-type mice. The degree of pulmonary inflammation was much less severe in GATA-3-overexpressing mice. The induction of T-bet and IFN-gamma genes was suppressed, but a significant induction of Th2 cytokines, including IL-5 and IL-13, was observed in the lungs of GATA-3-overexpressing mice after antigen exposure. Supplementation with recombinant IFN-gamma enhanced lung inflammatory responses in GATA-3-overexpressing mice to the level of wild-type mice. Because antigen-induced IFN-gamma production predominantly occurred in CD4+ T cells, nude mice were transferred with CD4+ T cells from either wild-type or GATA-3-overexpressing mice and subsequently exposed to antigen. Lung inflammatory responses were significantly lower in nude mice transferred with CD4+ T cells from GATA-3-overexpressing mice than in those with wild-type CD4+ T cells, with a reduction of lung IFN-gamma level.These results indicate that overexpression of GATA-3 attenuates the development of HP by correcting the Th1-polarizing condition.
- Published
- 2007