1. ORIGINAL ARTICLE: Endogenous Adenosine Down-Modulates Mid-Trimester IntraAmniotic Tumor Necrosis Factor-α Production
- Author
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Ann Marie Bongiovanni, Steven S. Witkin, Iara M. Linhares, Daniel W. Skupski, Uma Perni, and Devrim Sezen
- Subjects
medicine.medical_specialty ,Amniotic fluid ,medicine.diagnostic_test ,biology ,business.industry ,medicine.medical_treatment ,Immunology ,Obstetrics and Gynecology ,Interleukin ,Adenosine ,Adenosine deaminase ,Cytokine ,Endocrinology ,Reproductive Medicine ,Internal medicine ,medicine ,Amniocentesis ,biology.protein ,Immunology and Allergy ,Tumor necrosis factor alpha ,business ,Ex vivo ,medicine.drug - Abstract
Problem To determine whether adenosine in amniotic fluid down-regulates pro-inflammatory cytokine production. Method of study Mid-trimester amniotic fluid from 21 women was incubated ex vivo in the presence or absence of human adenosine deaminase, the enzyme that irreversibly degrades adenosine. After 24 hr, supernatants were assayed by ELISA for tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-10. Clinical parameters were obtained after completion of laboratory testing. Results Inclusion of adenosine deaminase resulted in a median increase in TNF-α production from 0.9 to 7.3 pg/mL (P = 0.0014). IL-6 production exhibited a non-significant median increase from
- Published
- 2009
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